A5102924169- Hippo pathway signaling and YAP/TAZ
- Influenza Virus Research Studies
- Renal and related cancers
- Immune Response and Inflammation
- Immune cells in cancer
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Lipid metabolism and biosynthesis
- Hepatitis B Virus Studies
- Kruppel-like factors research
- Medicinal Plants and Bioactive Compounds
- Venomous Animal Envenomation and Studies
- Immune responses and vaccinations
- interferon and immune responses
- Inflammasome and immune disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Tissue Engineering and Regenerative Medicine
- Curcumin's Biomedical Applications
- Nicotinic Acetylcholine Receptors Study
- Genetic Syndromes and Imprinting
Longgang Central Hospital
2025
Shenzhen Second People's Hospital
2022-2024
Shenzhen University
2024
Czech Academy of Sciences, Institute of Physics
2024
Chinese Academy of Sciences
2024
Zhejiang Shuren University
2024
Shanghai Public Health Clinical Center
2013-2023
Shanghai Medical College of Fudan University
2022
Chinese University of Hong Kong
2017-2022
Shenzhen University Health Science Center
2022
Peptidomics and genomics analyses were used to study an anti-infection array of peptides amphibian skin. 372 cDNA sequences antimicrobial characterized from a single individual skin the frog Odorrana grahami that encode 107 novel peptides. This contribution almost triples number currently reported The could be organized into 30 divergent groups, including 24 groups. diversity in peptide coding is, our knowledge, most extreme yet described for any animal. patterns diversification suggest...
T cells play a critical role in coronavirus diseases. How they do so COVID-19 may be revealed by analyzing the epigenetic chromatin accessibility of cis- and trans-regulatory elements creating transcriptomic immune profiles. We performed single-cell assay for transposase-accessible (scATAC) RNA (scRNA) sequencing (seq) on peripheral blood mononuclear (PBMCs) severely ill/critical patients (SCPs) infected with COVID-19, moderate (MPs), healthy volunteer controls (HCs). About 76,570 107,862...
The serine/threonine kinases MST1 and MST2 are core components of the Hippo pathway, which has been found to be critically involved in embryonic kidney development. Yes-associated protein (YAP) transcriptional coactivator with PDZ-binding motif (TAZ) pathway's main effectors. However, biologic functions Hippo/YAP pathway adult kidneys not well understood, functional role studied.We used immunohistochemistry examine expression mouse MST2, homologs Drosophila. We generated mice tubule-specific...
Hepatitis B virus exposure in children usually develops into chronic hepatitis (CHB). Although surface antigen (HBsAg)-specific CD8+ T cells contribute to resolve HBV infection, they are preferentially undetected CHB patients. Moreover, the mechanism for this rarely detected HBsAg-specific remains unexplored. We herein found that frequency of was inversely correlated with expansion monocytic myeloid-derived suppressor (mMDSCs) young rather than adult patients, and CCR9 upregulated by HBsAg...
The avian origin influenza A virus (IAV) H7N9 has caused a considerable number of human infections associated with high rates death since its emergence in 2013. As vital component the host innate immune system, nucleotide-binding domain leucine-rich repeat containing receptor, pyrin 3 (NLRP3) inflammasome plays critical role against H1N1 viral infection. However, function NLRP3 immunological responses to lethal is still obscure. Here, we demonstrated that mice deficient for components,...
Follistatin (FS)-like 1 (FSTL1) is a member of the FS-SPARC (secreted protein, acidic and rich in cysteine) family secreted extracellular matrix proteins. The functions FSTL1 have been studied heart lung injury as well wound healing; however, role kidney largely unknown. Here, we show using single-cell RNA-Seq that Fstl1 was enriched stromal cells obstructed mouse kidneys. In addition, immunofluorescence demonstrated expression induced fibroblasts during fibrogenesis mice human patients. We...
The importance of macrophages in kidney diseases has been well established; however, the mechanisms underlying infiltration into injured kidneys are not understood. RGMb is a member repulsive guidance molecule (RGM) family. can be expressed on cell surface but large portion localized intracellularly. Among various immune types, express highest levels RGMb, biological functions remain largely unknown. We find that promoted macrophage migration vitro and vivo, enhanced aggravated inflammation...
Neuraminidase inhibitors (NAIs) are the only available licensed therapeutics against human H7N9 influenza virus infections. The emergence of NAI-resistant variants H7N9viruses with an NA R292K mutation poses a therapeutic challenge. A comprehensive understanding susceptibility these viruses to clinically NAIs, non-NAIs and their combinations is crucial for effective treatment. In this study, by using limited serial passage plaque purification, variant Anhui1 lineage was isolated from patient...
Final urine volume and concentration are defined by water reabsorption through the channel proteins aquaporin (AQP)-2, -3 -4 in collecting duct. However, transcriptional regulation of these AQPs is not well understood. The Hippo/Yes-associated protein 1 (YAP) pathway plays an important role organ size control tissue homeostasis. When Hippo including Mst1/Mst2 kinases inhibited, YAP activated functions as a transcription co-activator. Our previous work revealed pathological tubular activation...
Abstract Background There are remarkable genetic differences between animal major histocompatibility complex (MHC) systems and the human leukocyte antigen (HLA) system. HLA transgenic humanized mouse model offer a much better method to study HLA‐A‐related principal mechanisms for vaccine development HLA‐A‐restricted responses against infection in human. Methods A recombinant gene encoding chimeric HLA‐A30 monochain was constructed. This HHD molecule contains following: α1‐α2 domains of...
Transmembrane protein 52B (TMEM52B), a newly identified tumor-related gene, has been reported to regulate various tumors, yet its role in nasopharyngeal carcinoma (NPC) remains unclear. Transcriptomic analysis of NPC cell lines reveals frequent overexpression TMEM52B, and immunohistochemical results show that TMEM52B is associated with advanced tumor stage, recurrence, decreased survival time. Depleting inhibits the proliferation, migration, invasion, oncogenesis cells vivo. encodes two...
A new avian-origin influenza virus (H7N9) recently crossed the species barrier and infected humans; therefore, there is an urgent need to establish mammalian animal models for studying pathogenic mechanism of this strain immunological response. In study, we attempted develop mouse H7N9 infection because mice are traditionally most convenient viruses. We showed that novel isolated from a patient could infect inbred BALB/c C57BL/6 as well outbred Institute Cancer Research (ICR) mice. The...
The synthesis of artificial cells is crucial for understanding the origins life. In synthetic cells, however, absence membrane-bound organelles and auxiliary proteins severely limits efficient expression precise localization membrane using cell-free systems. Here we introduce a robust method that significantly enhances protein by recruiting mRNA to phospholipid membranes. This achieved through use cholesterol-modified, single-stranded DNA anchors pairs with mRNA's untranslated region....
C12orf59 is a novel gene widely expressed in diverse normal human tissues. Aberrant expression of C12orf59, which involved tumor progression, has been reported few types cancer. However, its and biological function esophageal squamous cell carcinoma (ESCC) remain largely unclear. Here, we found that the mRNA protein levels were prominently higher both tissues most ESCC lines. Functionally, overexpression promoted proliferation, migration invasion, whereas depletion worked oppositely....
Abstract Background Staphylococcus aureus can cause serious infections by secreting many superantigen exotoxins in “carrier” or “pathogenic” states. HLA DQ and DR humanized mice have been used as a small animal model to study the role of two molecules during S. infection. However, contribution DP infection is unknown yet. Methods In this study, we produced DP401 DRA0101 microinjection C57BL/6J zygotes. Neo‐floxed IAβ +/− were crossbred with Ella‐Cre further HLA‐DRA0101 mice. After several...
Ganoderma lucidum polysaccharides (GLP) are one of the major bioactive components with many beneficial properties. In present study we aimed to systematically evaluate effects GLP on lipid metabolism in human (HPA-v) and murine adipocytes (3T3-L1). Cell viability was assessed by MTT assay. Lipid accumulation mature were evaluated ORO staining quantified using triglyceride (TG) Lipolysis investigated measuring free glycerol released cell culture medium after treatments. The mRNA protein...
Gram-negative bacteria are major pathogens that can cause illnesses in giant pandas. Lipopolysaccharides (LPS), components of bacteria, activate immune responses mammals (i.e., humans and mice) through recognition by toll-like receptors (TLRs). However, the pandas’ response to LPS stimulation differences between panda other not fully known. In this study, we administrated peripheral blood mononuclear cells (PBMCs) from pandas, humans, C57BL/6 mice, rhesus monkeys treatment at 6 h followed...