A5021967170- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Advanced Proteomics Techniques and Applications
- Immune Response and Inflammation
- Proteoglycans and glycosaminoglycans research
- Blood disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Invertebrate Immune Response Mechanisms
Macquarie University
2020-2024
IE University
2020
Myeloperoxidase (MPO) plays essential roles in neutrophil-mediated immunity via the generation of reactive oxidation products. Complex carbohydrates decorate MPO at discrete sites, but their functional relevance remains elusive. To this end, we have characterised structure-biosynthesis-activity relationship neutrophil (nMPO). Mass spectrometry demonstrated that nMPO carries both characteristic under-processed and hyper-truncated glycans. Occlusion Asn355/Asn391-glycosylation sites...
Neutrophils store microbicidal glycoproteins in cytosolic granules to fight intruding pathogens, but their granule distribution and formation mechanism(s) during granulopoiesis remain unmapped. Herein, we comprehensively profile the neutrophil N -glycoproteome with spatiotemporal resolution by analyzing four key types of intracellular organelles isolated from blood-derived neutrophils maturation bone marrow–derived progenitors using a glycomics-guided glycoproteomics approach. Interestingly,...
We recently discovered that human neutrophils express immunomodulatory glycoproteins carrying unusual and highly truncated paucimannosidic N-glycans (Man1-3GlcNAc2Fuc0-1), but their biosynthesis remains elusive. Guided by the well-characterized truncation pathway in invertebrates plants which N-acetyl-β-D-hexosaminidase (Hex) isoenzymes catalyze protein (PMP) formation, we here set out to test if homologous Hex α β subunits encoded HEXA HEXB drive a similar neutrophils. To this end,...
Aberrant protein glycosylation is a prominent cancer feature. While many tumour-associated glycoepitopes have been reported, advances in glycoanalytics continue to uncover new associations between and cancer. Guided by comprehensive literature survey suggesting that oligomannosylation (Man5-9 GlcNAc2) widespread often regulated glycosignature human cancers, we here revisit valuable compilation of nearly 500 porous graphitized carbon LC-MS/MS N-glycomics datasets acquired across 11 types...
Corticosteroid-binding globulin (CBG) delivers anti-inflammatory cortisol to inflamed tissues through proteolysis of an exposed reactive center loop (RCL) by neutrophil elastase (NE). We previously demonstrated that RCL-localized Asn347-linked N-glycans impact NE proteolysis, but a comprehensive structure-function characterization the RCL glycosylation is still required better understand CBG glycobiology. Herein, we first performed RCL-centric glycoprofiling serum-derived elucidate...
Abstract Tumor-associated macrophages (TAMs) form functionally diverse populations of innate immune cells in the tumor microenvironment (TME). Pro- and anti-inflammatory TAMs are central to cancer progression by shaping inflammation (im)balance, but it remains unknown if polarization-induced remodeling TAM glycocalyx critical for cellular communication occurs within TME. Taking a systems glycobiology approach, we here firstly used cell surface-focused glycomics lectin flow cytometry ex vivo...
Abstract Neutrophils store microbicidal glycoproteins in cytosolic granules to fight intruding pathogens, but their granule distribution and formation mechanism(s) during granulopoiesis remain unmapped. Herein, we perform comprehensive spatiotemporal N -glycoproteome profiling of isolated populations from blood-derived neutrophils maturation bone marrow-derived progenitors using glycomics-assisted glycoproteomics. Interestingly, the resting exhibited distinctive glycophenotypes including,...
Abstract Myeloperoxidase (MPO) plays essential roles in neutrophil-mediated immunity via the generation of reactive oxidation products. Complex carbohydrates decorate MPO at discrete sites, but their functional relevance remain elusive. To this end, we have characterised structure-biosynthesis-activity relationship neutrophil (nMPO). Mass spectrometry demonstrated that nMPO carries both characteristic under-processed and hyper-truncated glycans. Occlusion Asn355/Asn391-glycosylation sites...
Abstract Aberrant protein glycosylation is a prominent cancer feature. While many tumour-associated glycoepitopes have been reported, advances in glycoanalytics continue to uncover new associations between glycoproteins and cancer. Guided by comprehensive literature survey suggesting that oligomannosylation (Man 5-9 GlcNAc 2 , M5-M9) widespread albeit poorly studied glyco-signature human cancers, we here re-visit valuable compilation of nearly 500 LC-MS/MS N -glycomics datasets acquired...