A5101476829- Nitric Oxide and Endothelin Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antifungal resistance and susceptibility
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Receptor Mechanisms and Signaling
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Ectopic Pregnancy Diagnosis and Management
- Analytical Chemistry and Chromatography
- Photoreceptor and optogenetics research
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Pregnancy and Medication Impact
- interferon and immune responses
- Reproductive System and Pregnancy
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Ferroptosis and cancer prognosis
- Histone Deacetylase Inhibitors Research
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Adenosine and Purinergic Signaling
University of Oxford
2020-2024
Chinese University of Hong Kong
2024
Guangzhou University of Chinese Medicine
2020
Abstract G protein-coupled receptors (GPCRs) are cell-surface that respond to various stimuli induce signalling pathways across cell membranes. Recent progress has yielded atomic structures of key intermediates 1,2 and roles for lipids in 3,4 . However, capturing events a wild-type receptor real time, native membrane its downstream effectors, remained elusive. Here we probe the archetypal class A GPCR, rhodopsin, directly from fragments disc membranes using mass spectrometry. We monitor...
Genomic screening uncovered interferon-gamma (IFNγ) pathway defects in tumours refractory to immune checkpoint blockade (ICB). However, its non-mutational regulation and reversibility for therapeutic development remain less understood.
Myeloperoxidase (MPO) plays essential roles in neutrophil-mediated immunity via the generation of reactive oxidation products. Complex carbohydrates decorate MPO at discrete sites, but their functional relevance remains elusive. To this end, we have characterised structure-biosynthesis-activity relationship neutrophil (nMPO). Mass spectrometry demonstrated that nMPO carries both characteristic under-processed and hyper-truncated glycans. Occlusion Asn355/Asn391-glycosylation sites...
Glycoproteins are inherently heterogeneous and therefore resolving structures in their entirety remains a major challenge structural biology. Native mass spectrometry has transformed our ability to study glycoproteins, despite advances high-resolution instrumentation, there comparatively few studies demonstrating its potential with data largely limited an overall measure of monosaccharide composition for all glycans across glycosylation sites given protein. Clearly, these readouts lack...
Native mass spectrometry is now a well-established method for the investigation of protein complexes, specifically their subunit stoichiometry and ligand binding properties. Recent advances allowing analysis complex mixtures lead to an increasing diversity complexity in spectra obtained. These can be time-consuming tackle through manual assignment challenging automated approaches.
Problem To investigate the relation of inflammation-related parameters and pregnancy outcome in women with early threatened abortion. Method study 630 abortion were divided into two groups based on outcome. All them had blood routine examination before treating. The differences between analyzed by Chi-squared test, Student T Mann-Whitney U Binary Logistic Regression, Marginal Structural Model Threshold effect analysis. Results We found that there is no significant difference for NLR...
Abstract Background: Immune checkpoint blockade (ICB) therapies by antibodies have revolutionized the treatment paradigm for a variety of cancers. Although subsets people exhibit durable responses, resistance and relapse are common in hepatocellular carcinoma (HCC). We therefore aim to delineate mechanism underlying failure ICB therapy HCC, more importantly identify ICB-sensitizing therapeutic approaches. Methods: ICB-resistant orthotopic-grafted murine models were established via serial...
Abstract Myeloperoxidase (MPO) plays essential roles in neutrophil-mediated immunity via the generation of reactive oxidation products. Complex carbohydrates decorate MPO at discrete sites, but their functional relevance remain elusive. To this end, we have characterised structure-biosynthesis-activity relationship neutrophil (nMPO). Mass spectrometry demonstrated that nMPO carries both characteristic under-processed and hyper-truncated glycans. Occlusion Asn355/Asn391-glycosylation sites...