Single‐stranded guanosine‐rich oligodeoxyribonucleotides (GROs) have a propensity to form quadruplex structures that are stabilized by G‐quartets. In addition intense speculation about the role of G‐quartet formation in vivo, there is considerable interest therapeutic potential oligonucleotides as aptamers or non‐antisense antiproliferative agents. We previously described several GROs inhibit proliferation and induce apoptosis cancer cell lines. The activity these was related their ability...

Coiled-coil motifs provide simple systems for studying molecular self-assembly. We designed two 28-residue peptides to assemble into an extended coiled-coil fiber. Complementary interactions in the core and flanking ion-pairs were used direct staggered heterodimers. These had "sticky-ends" promote formation of long fibers. For comparison, we also synthesized a permuted version one peptide associate with other form canonical heterodimers "blunt-ends" that could not longitudinally. The...

Modifications of natural DNA in a cell-free medium by antitumor monodentate Ru(II) arene compounds the general formula [(η6-arene)Ru(en)Cl]+ (arene = biphenyl, dihydroanthracene, tetrahydroanthracene, p-cymene, or benzene; en ethylenediamine) were studied atomic absorption, melting behavior, transcription mapping, circular and linear dichroism, plasmid unwinding, competitive ethidium displacement, differential pulse polarography. The results indicate that these complexes bind preferentially...

Three point turn: A metallosupramolecular helicate with trigonal-antiprismatic geometry fits perfectly into the central hydrophobic cavity of a three-way DNA junction allowing new mode recognition.

The essential oil and gum of Pistacia lentiscus var. chia, commonly known as the mastic tree, are natural antimicrobial agents that have found extensive uses in medicine recent years. In this work, chemical composition was studied by GC−MS, majority their components identified. α-Pinene, β-myrcene, β-pinene, limonene, β-caryophyllene were to be major components. antibacterial activity 12 itself evaluated using disk diffusion method. Furthermore, attempts made separate into different...

Suitably functionalized dipeptides have been shown to be effective hydrogelators. The design of the hydrogelators and mechanism by which hydrogelation occurs are both currently not well understood. Here, we utilized hydrolysis glucono-δ-lactone gluconic acid as a means adjusting pH in naphthalene−alanylvaline solution allowing specific targeting final pH. In addition, this method allows assembly process characterized. We show that begins charge is removed from C-terminus dipeptide. removal...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnantiopreferential DNA binding of [ruthenium(II)(1,10-phenanthroline)3]2+ studied with linear and circular dichroismCatharina Hiort, Bengt Norden, Alison RodgerCite this: J. Am. Chem. Soc. 1990, 112, 5, 1971–1982Publication Date (Print):February 1, 1990Publication History Published online1 May 2002Published inissue 1 February 1990https://pubs.acs.org/doi/10.1021/ja00161a050https://doi.org/10.1021/ja00161a050research-articleACS PublicationsRequest...

Luminescent Ln−Pt2 metallohairpin complexes have been developed, and their intercalative recognition with DNA has demonstrated linear dichroism spectroscopy. The heterotrimetallic were formed in a one-step reaction, by assembly of an aminopolycarboxylate ligand, platinum terpyridine unit, the lanthanide salt. bear neutral moiety two positively charged platinum-containing intercalating units. Nd(III) analogues are luminescent near infrared, this near-IR luminescence is retained upon binding...

We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with binding constant in excess 10 7 M −1 and induces bending intramolecular coiling. The two enantiomers helical molecule bind differently to different structural effects. report characterization interactions by range biophysical techniques. binds dramatic is less for P enantiomer.

Abstract Gold nanoparticles (AuNPs) are promising materials for many bioapplications. However, upon contacting with biological media, AuNPs undergo changes. The interaction proteins results in the so‐called protein corona (PC) around AuNPs, leading to new bioidentity and optical properties. Understanding mechanisms of PC formation its functions can help us utilise benefits avoid drawbacks. To date, most previous works aimed understand governing focused on spherical nanoparticles, although...

In the groove! A tetracationic supramolecular cylinder, [Fe2L3]4+ (L=C25H20N4), with a triple-helical architecture is just right size to fit into major groove of DNA (see picture) and too big minor groove. NMR spectroscopic data confirm that cylinder binds in Linear dichroism shows very low loadings have dramatic bending effect on atomic force microscopy images show this an intramolecular resulting coils DNA.

Abstract A systematic theoretical study of the CD [poly(dA‐dT)] 2 and its complexes with achiral small molecules is presented. The spectra poly(dA):poly(dT) are calculated for various DNA structures using matrix method. experimental agree reasonably well but less poly (dA):poly (dT). spectrum [poly (dA‐dT)] fails to reproduce wavelength region 205–245 nm spectrum. This discrepancy can be explained by a magnetic dipole allowed transition contributing significantly in this region. induced...

Oligonucleotide-based therapies have considerable potential in cancer, viral, and cardiovascular disease therapies. However, it is becoming clear that the biological effects of oligonucleotides are not solely due to intended sequence-specific interactions with nucleic acids. Oligonucleotides also capable interacting numerous cellular proteins owing their polyanionic character or specific secondary structure. We examined antiproliferative activity, protein binding, G-quartet formation a...

For ligand-biomacromolecule titration experiments it has been traditional practice to extract parameters such as the equilibrium binding constant K and number of bases per ligand site n with relatively labour intensive methods, usually based on single wavelength data, difference method by Rodger Nordén coupled together a Scatchard plot. Presented in this paper are both theory least squares fitting derive more directly from all spectral non-linear experimental data. Both case non competitive...

Primordium formation of Agaricus bisporus depends on the presence a casing layer containing stimulatory bacteria and sufficient air exchange. The influence specific pseudomonad populations volatile organic compounds (VOC) primordium A. was studied in microcosm cultures. VOC produced by mycelium were predominantly C8 compounds, some which could inhibit formation, with 1-octen-3-ol being most inhibitory. A rye grain substrate, 2-ethyl-1-hexanol, grown also inhibited formation....