A5063654523- Clinical Laboratory Practices and Quality Control
- Protein Structure and Dynamics
- Hormonal and reproductive studies
- Pharmacological Effects and Assays
- Chronic Kidney Disease and Diabetes
- Bacterial Genetics and Biotechnology
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Dialysis and Renal Disease Management
- Molecular spectroscopy and chirality
- Risk and Safety Analysis
- Muscle metabolism and nutrition
- Lipoproteins and Cardiovascular Health
- Healthcare cost, quality, practices
- Photosynthetic Processes and Mechanisms
- Renal Diseases and Glomerulopathies
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Pharmaceutical studies and practices
- Meta-analysis and systematic reviews
- Bacteriophages and microbial interactions
- Random lasers and scattering media
- Biosimilars and Bioanalytical Methods
- Software Reliability and Analysis Research
- Field-Flow Fractionation Techniques
University Hospitals Birmingham NHS Foundation Trust
2020-2025
Healthcare Quality Improvement Partnership
2019
Heart of England NHS Foundation Trust
2010-2012
Heartlands Hospital
2010-2011
University of Warwick
2002-2007
Single‐stranded guanosine‐rich oligodeoxyribonucleotides (GROs) have a propensity to form quadruplex structures that are stabilized by G‐quartets. In addition intense speculation about the role of G‐quartet formation in vivo, there is considerable interest therapeutic potential oligonucleotides as aptamers or non‐antisense antiproliferative agents. We previously described several GROs inhibit proliferation and induce apoptosis cancer cell lines. The activity these was related their ability...
Long molecules such as fibrous proteins are particularly difficult to characterise structurally. We have recently designed a microvolume Couette flow linear dichroism (LD) cell whose sample volume is only 20–40 µL in contrast previous cells where the of required has typically been order 1000–2000 µL. This brings requirements LD level it can be used for biological samples. Since difference absorption light polarised parallel an orientation direction and perpendicular that direction, ideal...
The first page of this article is displayed as the abstract.
As one of the most requested profiles blood tests, there is a need for standardization among liver function tests (LFT). Alanine aminotransferase (ALT) and aspartate (AST) are key markers hepatocellular injury. ALT AST used to calculate Fibrosis-4 (FIB-4) score assessing fibrosis. Despite recommendations by International Federation Clinical Chemistry (IFCC) include pyridoxal-5-phosphate in assay methodologies, laboratories continue omit this.
Polymer formation by the essential FtsZ protein plays a crucial role in cytokinesis of most prokaryotes. Lateral associations between these polymers to form bundles or sheets are widely predicted be extremely important for function vivo. We have carried out study vitro polymer and bundling using linear dichroism (LD) assess structural properties polymers. demonstrate proof-of-principle experiments show that LD can used as technique follow polymerization, we present spectra Our subsequent...
Introduction A number of guidance documents have been published in recent years for the diagnosis and management hypogonadism (HG). Laboratory practice has a major role supporting guidelines with accurate precise serum total testosterone (TT) methods standardised pre- post-analytical protocols. Our study investigated whether laboratory currently supports HG. Methods An internet-based questionnaire survey senior biochemists (UK/Republic Ireland) was conducted (April-May 2018). Questions...
Abstract Objectives Laboratories need to take into consideration the specificity and imprecision of assays not only in verification, but also quality assessment. This study investigates composition serum used EQA materials by comparing material from a single multiple donors (pooled material), across methods, using creatinine as an example. Methods Sixteen different matrices were distributed 36 specimens through UK NEQAS for Acute Chronic Kidney Disease Scheme March 2022 2023. Male-only...
Background: Inter-assay variation between different immunoassays and mass spectrometry methods hampers the biochemical confirmation of male hypogonadism. Furthermore, some laboratories utilis eassay manufacturer reference ranges that do not necessarily mirror assay performance characteristics, with lower limit normality ranging from 4.9 nmol/L to 11 nmol/L. The quality normative data underlying commercial immunoassay is uncertain.Design: A working group reviewed published evidence agreed...
The Tat system catalyzes the transport of folded globular proteins across bacterial plasma membrane and chloroplast thylakoid. It recognizes cleavable signal peptides containing a critical twin‐arginine motif but little is known overall structure these peptides. In this report, we have analyzed secondary SufI peptide, together with those two nonfunctional variants in which region around twin‐arginine, RRQFI, replaced by KKQFI or RRQAA. Circular dichroism studies show that peptide exists as...
Abstract A thermostatted micro volume Couette cell has been designed to enable linear dichroism ( LD ) data be collected at a range of temperatures. The is development the traditional flow and includes recent micro‐volume (20–40 μL) coupled with addition heating element, temperature probe controller. This new opens way not only analysis systems where sample critical, but also for sensitive samples. polymerization microtubule protein tubulin followed in different conditions using cell....
Abstract Both real and synthetic materials are regularly used by providers of external quality assessment proficiency testing schemes, there are, in most cases, justifiable reasons for the choice made. This article focusses on field clinical biochemistry discusses different properties a material benefits limitations using or material. However, overarching principles should be able to applied all sectors within laboratory. Whilst genuine/real would appear preferred matrix, this is not always...
Stability of concentrations urinary stone-related metabolites was analyzed from samples recurrent stone formers to assess necessity and effectiveness urine acidification during collection storage.