A5017253735- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Antimicrobial Peptides and Activities
- Pharmacogenetics and Drug Metabolism
- Cell Adhesion Molecules Research
- Inflammasome and immune disorders
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Neuropeptides and Animal Physiology
- Nitric Oxide and Endothelin Effects
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Oral microbiology and periodontitis research
- Blood disorders and treatments
- Immune cells in cancer
- Antifungal resistance and susceptibility
- Phagocytosis and Immune Regulation
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Computational Drug Discovery Methods
- Bacterial biofilms and quorum sensing
- Asthma and respiratory diseases
- Receptor Mechanisms and Signaling
- Helicobacter pylori-related gastroenterology studies
University of Gothenburg
2016-2025
Göteborgs Stads
2024
Roche (Switzerland)
2015-2020
Swedish Academy
2019
Medivir (Sweden)
2018-2019
Uppsala University
1996-2014
AstraZeneca (Sweden)
2012-2014
AstraZeneca (United States)
2014
Karolinska Institutet
2014
AstraZeneca (United Kingdom)
2011-2013
SMA is an inherited disease that leads to loss of motor function and ambulation a reduced life expectancy. We have been working develop orally administrated, systemically distributed small molecules increase levels functional SMN protein. Compound 2 was the first SMN2 splicing modifier tested in clinical trials healthy volunteers patients. It safe well tolerated increased protein up 2-fold Nevertheless, its development stopped as precautionary measure because retinal toxicity observed...
Galectin-3, a β-galactoside binding, endogenous lectin, takes part in various inflammatory events and is produced substantial amounts at foci. We investigated whether extracellular galectin-3 could participate the phagocytic clearance of apoptotic neutrophils by macrophages, process crucial importance for termination acute inflammation. Using human leukocytes, we show that exogenously added increased uptake monocyte-derived macrophages (MDM). Both proportion MDM engulfed prey number each...
The human cathelicidin peptide, LL-37, is a host defense peptide with wide range of immunomodulatory activities and modest direct antimicrobial properties. LL-37 can exert both pro- anti-inflammatory effects modulate the proinflammatory responses peripheral blood monocytes epithelial cells. In this study, we evaluated effect on mouse bone marrow-derived macrophages (BMDM) tissue in vitro vivo. dramatically reduced TNF-α NO levels produced by LPS IFN-γ-polarized M1-BMDM slightly reactive...
Neutrophils enter sites of infection, where they can eliminate pathogenic bacteria in an oxidative manner. Despite their predominance active tuberculosis lesions, the function neutrophils this important human infection is still highly controversial. We observed that virulent Mycobacterium survived inside despite prompt activation these defence cells' microbicidal effectors. Survival M. was accompanied by necrotic cell death infected neutrophils. Necrotic entirely depended on radical oxygen...
Polymorphonuclear neutrophils (PMN) are potent inflammatory effector cells essential to host defense, but at the same time they may cause significant tissue damage. Thus, timely induction of neutrophil apoptosis is crucial avoid damage and induce resolution inflammation. NK have been reported influence innate adaptive immune responses by multiple mechanisms including cytotoxicity against other cells. In this study, we analyzed effect interaction between neutrophils. Coculture experiments...
Infection with Helicobacter pylori causes chronic gastritis, which is characterized by a dense mucosal infiltration inflammatory cells such as monocytes/macrophages. H. pylori–induced inflammation risk factor for the development of gastric adenocarcinoma, but mechanisms involved in pylori–associated carcinogenesis are poorly understood. A cecropin-like peptide, Hp(2-20), was found to be monocyte chemoattractant and activated NADPH-oxidase produce oxygen radicals. The receptors mediating...
Reactive oxygen species (ROS) generated by the cellular NADPH-oxidase are crucial for phagocytic killing of ingested microbes and have been implicated as signaling molecules in various processes. For example, ROS thought to be involved activation transcription factor NF-kappaB, central mediating production proinflammatory cytokines response inflammatory stimuli. Several studies demonstrated that inhibitors interfere with NF-kappaB cytokines. Curiously, patients chronic granulomatous disease...
Bacterial colonization of the lower respiratory tract is frequently seen in chronic obstructive pulmonary disease (COPD), and may cause exacerbations leading to progression. Antimicrobial peptides comprise an important part innate lung immunity, not least cathelicidin human cationic antimicrobial protein-18/LL-37. Peptidylarginine deiminases (PADIs) post-translationally modify proteins by converting peptidylarginine residues neutral peptidylcitrulline. An increased presence PADI2...
Neutrophil heterogeneity was described decades ago, but it could not be elucidated at the time whether existence of different neutrophil subsets had any biological relevance. It has been corroborated in recent years that subsets, defined by differential expression various markers, are indeed present human blood, calling for renewed attention to this question. The granule protein olfactomedin 4 (OLFM4) suggested define two such subsets. We confirm simultaneous presence one OLFM4-positive and...
Sepsis is a potentially deadly disease that often caused by gram-positive bacteria, in particular Staphylococcus aureus (S. aureus). As there are few effective therapies for sepsis, increased basic knowledge about factors predisposing needed.The purpose of this study was to the effect Western diet on mortality induced intravenous S. inoculation and immune functions before after bacterial inoculation. Here we show C57Bl/6 mice high-fat (HFD) 8 weeks, like genetically obese Ob/Ob low-fat...
Systematic under-prediction of clearance is frequently associated with in vitro kinetic data when extrapolated using physiological scaling factors, appropriate binding parameters and the well-stirred model. The present study describes a method removing this systematic bias through application empirical correction factors derived from regression analyses applied to vivo for defined set reference compounds.Linear lines were established intrinsic (CLint), scaled isolated hepatocyte incubations....
Staphylococcus aureus biofilms, a leading cause of persistent infections, are highly resistant to immune defenses and antimicrobial therapies. In the present study, we investigated contribution fibrin staphylokinase (Sak) biofilm formation. both clinical S. isolates laboratory strains, high Sak-producing strains formed less than that lacked Sak, suggesting Sak prevents addition, induced detachment mature biofilms. This effect depended on plasminogen activation by Sak. Host-derived fibrin,...
Galectin-3 is a 32 kDa protein secreted by macrophages involved in processes such as cell activation, chemotaxis and phagocytosis. has previously been shown to improve the ability of airway ingest apoptotic cells (efferocytosis) chronic obstructive pulmonary disease (COPD) may be interest non-eosinophilic asthma (NEA) which also characterised impaired efferocytosis. It was hypothesised that addition exogenous galectin-3 monocyte-derived (MDMs) derived from donors with NEA would enhance their...
Abstract Polymorphonuclear neutrophils (PMNs) are innate effector cells with pivotal roles in pathogen recognition, phagocytosis, and eradication. However, their role the development of subsequent immune responses is incompletely understood. This study aimed to identify mechanisms relevance cross talk between human NK its potential promoting adaptive immunity. TLR-stimulated PMNs were found release soluble mediators attract activate vitro. PMN-conditioned displayed enhanced cytotoxicity...