A5061511792- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- IL-33, ST2, and ILC Pathways
- Monoclonal and Polyclonal Antibodies Research
- Parasites and Host Interactions
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- Helicobacter pylori-related gastroenterology studies
- Folate and B Vitamins Research
- Rheumatoid Arthritis Research and Therapies
- Cytomegalovirus and herpesvirus research
- Peroxisome Proliferator-Activated Receptors
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- Influenza Virus Research Studies
- Chronic Lymphocytic Leukemia Research
- Ion Transport and Channel Regulation
- Inflammatory Bowel Disease
- Gestational Diabetes Research and Management
- Otitis Media and Relapsing Polychondritis
- Cancer Immunotherapy and Biomarkers
Karolinska Institutet
2012-2023
Trinity College Dublin
2010-2018
University College Cork
2018
Armauer Hansen Research Institute
2015
St. James's Hospital
2011-2015
Inserm
2013
University of Gothenburg
2005-2012
Molecular Medicine Ireland
2010
Institute of Molecular Medicine
2010
Sahlgrenska University Hospital
2009
Clostridium difficile is the etiological agent of antibiotic-associated diarrhoea (AAD) and pseudomembranous colitis in humans. The role surface layer proteins (SLPs) this disease has not yet been fully explored. aim study was to investigate a for SLPs recognition C. subsequent activation immune system. Bone marrow derived dendritic cells (DCs) exposed were assessed production inflammatory cytokines, expression cell markers their ability generate T helper (Th) responses. DCs isolated from...
Abstract T follicular helper (TFH) cells are critical initiators in the development of cell-dependent humoral immunity and generation protective immunity. We demonstrate that TFH cell accumulation Ab production negatively regulated by B7-H1 (programmed death ligand 1) response to both helminth infection active immunization. Following immunization B7-H1−/− mice with keyhole limpet hemocyanin or Ags, there is a profound increase induction as result increased cycling decreased apoptosis...
Abstract Introduction In the present study we evaluated changes in B cell phenotype peripheral blood and bone marrow (BM) of patients with rheumatoid arthritis (RA) following anti-CD20 treatment using rituximab. Methods Blood BM samples were obtained from 37 RA prior to rituximab treatment. Ten these resampled 1 month rituximab, 14 after 3 months remaining 13 included long-term follow up. populations characterized by CD27/IgD/CD38/CD24 expression. Results One three retained up 30% cells...
Sepsis is a potentially deadly disease that often caused by gram-positive bacteria, in particular Staphylococcus aureus (S. aureus). As there are few effective therapies for sepsis, increased basic knowledge about factors predisposing needed.The purpose of this study was to the effect Western diet on mortality induced intravenous S. inoculation and immune functions before after bacterial inoculation. Here we show C57Bl/6 mice high-fat (HFD) 8 weeks, like genetically obese Ob/Ob low-fat...
The incidence of allergy and asthma in developed countries is on the increase this trend looks likely to continue. CD4+ T helper 2 (Th2) cells are major drivers these diseases their commitment controlled by cytokines such as interleukin 4, which turn regulated suppressor cytokine signaling (SOCS) proteins. We report that SOCS2−/− show markedly enhanced Th2 differentiation. mice, well RAG-1−/− mice transferred with cells, exhibit elevated type responses after helminth antigen challenge....
<h3>Objective</h3> Bile acids are important regulators of intestinal physiology, and the nuclear bile acid receptor, farnesoid X receptor (FXR), is emerging as a promising therapeutic target for several disorders. Here, we investigated role FXR in regulating fluid electrolyte transport potential agonists treating diarrhoeal diseases. <h3>Design</h3> Electrogenic ion was measured changes short-circuit current across voltage-clamped T<sub>84</sub> cell monolayers or mouse tissues Ussing...
Objective. To investigate whether intrauterine growth retardation (IUGR) and preterm delivery in a poor population of South Asia was associated with altered maternal fetal levels folate, vitamin B12, homocysteine. Subjects methods. Hundred twenty‐eight pregnant women from low socio‐economic strata the city Lahore, Pakistan were followed ultrasound 12th week pregnancy. Blood samples drawn woman cord at delivery. Serum analyzed by chemiluminescent immunoassay for folate B12 fluorescence...
We have shown that human CD20(+)25(+) B cells display immunomodulatory properties. The aim of this study was to investigate if CD25(+) are found within the CD27 memory cell population, and analyse pattern their cytokine production. isolated from healthy subjects, rheumatoid arthritis (RA) systemic lupus erythematosus (SLE) patients were analysed regarding frequency certain subsets. Purified subject used in vitro evaluate production cytokines. In subjects majority (60%) (CD20(+)27(+)) also...
Abstract Introduction Rheumatoid arthritis (RA) is frequently complicated with infections. The aim of our study was to evaluate vaccination response in patients RA after B-cell depletion by using rituximab. Methods Influenza (Afluria) and pneumococcal polysaccharides (Pneumo23) vaccines were given 6 months rituximab (post-RTX group, n = 11) or days before treatment (pre-RTX group; 8). never exposed RTX composed the control group (n 10). Vaccine-specific cellular responses evaluated on day...
B cells have been described as having the capacity to regulate cellular immune responses and suppress inflammatory processes. One such regulatory B-cell population is defined IL-10-producing CD19(+) CD1d(hi) cells. Previous work has identified an expansion of these in mice infected with helminth, Schistosoma mansoni. Here, microarray analysis from S. mansoni demonstrated significantly increased Tlr7 expression, while uninfected also elevated expression. Using IL-10 reporter, Il10(-/-)...
Intestinal epithelial barrier disruption is a feature of inflammatory bowel disease (IBD), but whether precedes or merely accompanies inflammation remains controversial. Tight junction (TJ) adhesion complexes control integrity. Since some TJ proteins reside in cholesterol-enriched regions the cell membrane termed lipid rafts, we sought to elucidate relationship between rafts and intestinal function. Lipid were isolated from Caco-2 cells primed with proinflammatory cytokine interferon-γ...
Aged individuals respond poorly to vaccination and have a higher risk of contracting infections in comparison younger individuals; whether age impacts on the composition function B cell subpopulations relevant for immune responses is still controversial. It also not known increased during HIV-1 infection further synergizes with virus alter subpopulations. In view number infected patients living high as result anti-retroviral treatment this an important issue clarify.In work we evaluated...
B cells are an important part of both innate and adaptive immune system. Their ability to produce antibodies, cytokines present antigen makes them a crucial in defence against pathogens. In this study, we have naïve Naval Medical Research Institute mice functionally characterized subpopulation splenic expressing CD25, which comprise about 1% the total cell compartment. Murine spleen were sorted into two highly purified populations either CD19(+) CD25(+) or CD25(-). We found that secreted...
Despite high vaccination coverage, pertussis is still a global concern in infant morbidity and mortality, improved vaccines are needed. A live attenuated Bordetella strain, named BPZE1, was designed as an intranasal vaccine candidate has recently been tested man phase I clinical trial. Here, we report the evaluation of B-cell responses after with BPZE1. Forty-eight healthy males no previous pertussis-vaccination were randomized into one three dose-escalating groups or placebo group. Plasma...
Early initiation of antiretroviral therapy (ART) is becoming a common clinical practice according to current guidelines recommending treatment all HIV-1-infected patients. However, it not known whether ART initiated during the early phase infection prevents establishment abnormal phenotypic features previously reported in CD4+ and CD8+T cells chronic HIV-1 infection. In this cross-sectional study, blood specimens were obtained from 17 patients who began shortly after (early [EA]),...
The helminth Schistosoma mansoni modulates the infected host's immune system to facilitate its own survival, by producing excretory/secretory molecules that interact with a variety of cell types including those system. Herein, we characterise S. adult male worm secretome and identify 111 proteins, 7 vaccine candidates several potential immunomodulatory activity. Amongst present in secretome, 17-19kDa protein analogous human cyclophilin A was identified. Given ability modulate regulating...
Abstract B cells play an important role in the development of autoimmune diseases due to their production autoantibodies, antigen‐presenting capacity and pro‐inflammatory cytokines. The purpose present study was analyse from rheumatoid arthritis (RA) systemic lupus erythematosus (SLE) patients, with respect expression IL‐2 receptor (IL‐2R) subunit CD25. Using flow cytometry, we found that CD25 + RA patients expressed significantly higher frequencies CD122 CD132 than control subjects,...