A5106721430- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Gene Regulatory Network Analysis
- Axon Guidance and Neuronal Signaling
- Immune Cell Function and Interaction
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Asthma and respiratory diseases
- Immune cells in cancer
- IL-33, ST2, and ILC Pathways
- Probiotics and Fermented Foods
- interferon and immune responses
- Neurobiology and Insect Physiology Research
- Aortic aneurysm repair treatments
- Antimicrobial Peptides and Activities
- Cancer, Hypoxia, and Metabolism
- Bioactive Compounds and Antitumor Agents
- Diet and metabolism studies
- Endoplasmic Reticulum Stress and Disease
- Tryptophan and brain disorders
- Macrophage Migration Inhibitory Factor
- Glioma Diagnosis and Treatment
- Ion Channels and Receptors
- Pediatric health and respiratory diseases
- Inflammatory mediators and NSAID effects
Paris Cardiovascular Research Center
2021-2024
Université Paris Cité
2021-2024
Inserm
2021-2024
Institut Cochin
2021-2022
Centre National de la Recherche Scientifique
2021-2022
New York University
2017
Columbia University
2014
Queen's University Belfast
2010-2013
Icahn School of Medicine at Mount Sinai
2012
University of Michigan
2010
Antimicrobial peptides (AMPs), including defensins and cathelicidins, constitute an arsenal of innate regulators paramount importance in the gut. The intestinal epithelium is exposed to myriad enteric pathogens these endogenous are essential fend off microbes protect against infections. It becoming increasingly evident that AMPs shape composition commensal microbiota help maintain homeostasis. They contribute immunity, hence playing important functions health disease. AMP expression tightly...
Store-operated Ca2+ entry (SOCE) through release-activated (CRAC) channels is critical for lymphocyte function and immune responses. CRAC are hexamers of ORAI proteins that form the channel pore, but contributions individual homologues to not well understood. Here we show deletion Orai1 reduces, whereas Orai2 increases, SOCE in mouse T cells. These distinct effects due ability ORAI2 heteromeric with ORAI1 attenuate function. The combined abolishes strongly impairs cell In vivo, Orai1/Orai2...
SLIT2 is a secreted polypeptide that guides migration of cells expressing Roundabout 1 and 2 (ROBO1 ROBO2) receptors. Herein, we investigated SLIT2/ROBO signaling effects in gliomas. In patients with glioblastoma (GBM), expression increased malignant progression correlated poor survival immunosuppression. Knockdown mouse glioma patient-derived GBM xenografts reduced tumor growth rendered tumors sensitive to immunotherapy. Tumor cell knockdown inhibited macrophage invasion promoted cytotoxic...
Tryptophan (Trp) is an essential amino acid, whose metabolism a key gatekeeper of intestinal homeostasis. Yet, its systemic effects, particularly on atherosclerosis, remain unknown. Here we show that high-fat diet (HFD) increases the activity indoleamine 2, 3-dioxygenase 1 (IDO), which shifts Trp from production microbiota-derived indole metabolites towards kynurenine production. Under HFD, specific deletion IDO in epithelial cells leads to inflammation, impaired barrier, augmented lesional...
The incidence of allergy and asthma in developed countries is on the increase this trend looks likely to continue. CD4+ T helper 2 (Th2) cells are major drivers these diseases their commitment controlled by cytokines such as interleukin 4, which turn regulated suppressor cytokine signaling (SOCS) proteins. We report that SOCS2−/− show markedly enhanced Th2 differentiation. mice, well RAG-1−/− mice transferred with cells, exhibit elevated type responses after helminth antigen challenge....
Abstract Background Mature blood cells arise from hematopoietic stem in the bone marrow by a process of differentiation along one several different lineage trajectories. This is often represented as series discrete steps increasing progenitor cell commitment to given lineage, but for general, whether instructive or stochastic remains controversial. Here, we examine this question analyzing single-cell transcriptomic data human cells, assessing cell-to-cell variability trajectories into four...
Abstract JAK2V617F mutation is associated with an increased risk for athero-thrombotic cardiovascular disease, but its role in aortic disease development and complications remains unknown. In a cohort of patients myeloproliferative neoplasm, was identified as independent factor dilation both the ascending descending thoracic aorta. Using single-cell RNA-seq, complementary genetically-modified mouse models, well pharmacological approaches, we found that pathogenic pro-inflammatory phenotype...
Suppressor of cytokine signaling (SOCS) proteins are key regulators CD4(+) T cell differentiation, and in particular, we have recently shown that SOCS2 inhibits the development Th2 cells allergic immune responses. Interestingly, transcriptome analyses identified as being preferentially expressed both natural regulatory (Tregs) inducible Tregs (iTregs); however, role Foxp3(+) Treg function or has not been fully elucidated. In this study, show despite having no effect on function, is highly...
Abstract SLIT2 is a secreted polypeptide that guides migration of cells expressing ROBO1&2 receptors. Herein, we investigated SLIT2/ROBO signaling effects in gliomas. In patients with glioblastoma (GBM), expression increased malignant progression and correlated poor survival immunosuppression. Knockdown mouse glioma patient derived GBM xenografts reduced tumor growth synergized immunotherapy to prolong survival. Tumor cell knockdown inhibited macrophage invasion promoted cytotoxic gene...
Interleukin-21 is a type I cytokine produced by CD4+ T cells and NK that regulates the function of both lymphoid non-lymphoid target cells.Although IL-21 has positive effects on development antibody-mediated cell-mediated immune responses, it also exerts some inhibitory lymphocytes dendritic cells.The specific actions mediated presumably can depend direct receptor-bearing as well indirect ability to affect production other cytokines.In mouse model systemic lupus erythematosus, we previously...
Abstract Hematopoietic differentiation has been metaphorically represented as linear trajectories with discrete steps from hematopoietic stem cells to mature cells. While the transcriptional state of at beginning or end these are well described bulk analysis, what happens in intermediate states remained elusive until use single cell approaches. Applying Shannon entropy measure cell-to-cell variability among same stage differentiation, we observed a transient peak gene expression all...