A5101540200- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Microscopic Colitis
- CAR-T cell therapy research
- Immune Response and Inflammation
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Celiac Disease Research and Management
- IL-33, ST2, and ILC Pathways
- MicroRNA in disease regulation
University of Genoa
1989-2016
Inserm
2000-2011
Centre de Recherche en Cancérologie de Marseille
2011
Centre National de la Recherche Scientifique
2000
Hôpital Laennec
2000
Hôpital Saint Lazare
2000
Hôpital Necker-Enfants Malades
2000
Hôpital Saint-Louis
2000
Celiac disease is a gluten-induced enteropathy characterized by the presence of gliadin-specific CD4(+) T cells in lamina propria and prominent intraepithelial T-cell infiltration unknown mechanism. The aim this study was to characterize subset(s) lymphocytes (IELs) expanding during active celiac provide insights into mechanisms involved their expansion.Flow-cytometric analysis isolated IELs and/or immunohistochemical staining frozen sections were performed 51 patients 50 controls with panel...
Abstract Breast cancer is the leading cause of death for women between ages 35 to 65. This mostly due intertumor heterogeneity and lack specific therapies all subtypes. However, some breast cancers with an unexpected good prognosis are associated enhanced antitumor immunity in situ. We studied whether subtypes might have different susceptibilities natural killer (NK) cells' immunity. collected a large public set microarray data primary tumors determined NK cell ligand expression. found that...
Polymorphonuclear neutrophils (PMN) are potent inflammatory effector cells essential to host defense, but at the same time they may cause significant tissue damage. Thus, timely induction of neutrophil apoptosis is crucial avoid damage and induce resolution inflammation. NK have been reported influence innate adaptive immune responses by multiple mechanisms including cytotoxicity against other cells. In this study, we analyzed effect interaction between neutrophils. Coculture experiments...
Abstract A novel family of inhibitory co‐receptors has been recently defined according to the presence in their intracytoplasmic domain immunoreceptor tyrosine‐based inhibition motifs (ITIM). In particular, this includes a low‐affinity receptor for IgG, FcγRIIB, which is widely expressed on hematopoietic cells, as well killer cell receptors (KIR) major histocompatibility complex (MHC) class I proteins, both T and natural (NK) lymphocytes. FcγRIIB KIR function depends upon tyrosine...
Abstract Polymorphonuclear neutrophils (PMNs) are innate effector cells with pivotal roles in pathogen recognition, phagocytosis, and eradication. However, their role the development of subsequent immune responses is incompletely understood. This study aimed to identify mechanisms relevance cross talk between human NK its potential promoting adaptive immunity. TLR-stimulated PMNs were found release soluble mediators attract activate vitro. PMN-conditioned displayed enhanced cytotoxicity...
Abstract We have analyzed the morphological characteristics of human T lymphocytes bearing CD3‐associated cell receptor (TcR) γ and δ chains. BB3 δ‐TCS1 monoclonal antibodies (mAb) were used to identify two distinct, nonoverlapping populations TcR γ/δ + cells which express products V 2 1 gene segments, respectively. In peripheral blood, most (δTCS‐1 ) non‐disulfide‐linked form whereas (BB3 disulfide‐linked form. The majority cloned exhibit a growth pattern different from that conventional...
Background: PD-1 is an immunological checkpoint that limits immune responses by delivering potent inhibitory signals to T cells upon interaction with specific ligands expressed on tumor/virus-infected cells, thus contributing escape mechanisms (1). Therapeutic blockade has been shown mediate tumor eradication impressive clinical results. Little known the expression/function of human NK (2). Objective: To clarify whether may express and analyze their phenotypic/functional features. Methods:...
In this study the phenotype and function of tumor-associated NK cells from perito- neal fluids a selected cohort patients with seropapillary ovarian carcinoma were analyzed. >50% these expression activating receptor NKp30 (1) in was substantially reduced as compared to autolo- gous peripheral blood cells. The impaired associ- ated presence one its cellular ligands (B7-H6) (2), which detectable surface/cytosolic molecule tumor soluble peri- toneal fluid. expressing NKp30low displayed an IFNγ...
<p>PDF file - 24K, Breast tumor cells killing in an autologous settings. NK-cells (after overnight incubation IL2/IL15) were tested for direct cytotoxic activity against Epcam+ (E/T = 2:1, n 3 donors). The percentage of positive CD107 is represented.</p>
<p>PDF file - 75K, FVB control mice (n=6, light grey bars) and MMTV-Neu dark were followed from 3 months of age until tumor occurrence, a Kaplan-Meier curve was established to determine tumor-free survival. Arrows indicate when the samples collected. Mice sacrificed during last sample (VI).</p>