A5058875000- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Melanoma and MAPK Pathways
- IL-33, ST2, and ILC Pathways
- Galectins and Cancer Biology
- IgG4-Related and Inflammatory Diseases
- Protein Tyrosine Phosphatases
- Cytokine Signaling Pathways and Interactions
- Immunodeficiency and Autoimmune Disorders
- Eosinophilic Esophagitis
- COVID-19 Clinical Research Studies
- Neuroendocrine Tumor Research Advances
- Reproductive System and Pregnancy
- Autoimmune and Inflammatory Disorders Research
- Synthesis and biological activity
- Gastrointestinal disorders and treatments
- Cell Adhesion Molecules Research
- Influenza Virus Research Studies
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
Assistance Publique Hôpitaux de Marseille
2012-2025
Inserm
2015-2024
Centre d’Immunologie de Marseille-Luminy
2013-2024
Centre National de la Recherche Scientifique
2015-2024
Aix-Marseille Université
2015-2024
Hôpital de la Timone
2018-2024
Centre de Résonance Magnétique Biologique et Médicale
2023
Hôpital de la Conception
2005-2020
Centre de Référence Déficits Immunitaires Héréditaires
2019
Service de la Santé Publique
2017
Natural killer (NK) cells are lymphocytes of the innate immune system that involved in early defenses against foreign cells, as well autologous undergoing various forms stress, such microbial infection or tumor transformation. NK cell activation is controlled by a dynamic balance between complementary and antagonistic pathways initiated upon interaction with potential target cells. express an array activating surface receptors can trigger cytolytic programs, cytokine chemokine secretion....
Dendritic cell-derived exosomes (Dex) are small extracellular vesicles secreted by viable dendritic cells. In the two phase-I trials that we conducted using first generation of Dex (IFN-γ-free) in end-stage cancer, reported exerted natural killer (NK) cell effector functions patients. A second (IFN-γ-Dex) was manufactured with aim boosting NK and T immune responses. We carried out a phase II clinical trial testing benefit IFN-γ-Dex loaded MHC class I- II-restricted cancer antigens as...
Coronavirus disease 2019 (COVID-19) is a caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has resulted in pandemic1. The C5a complement factor its receptor C5aR1 (also known as CD88) have key role the initiation maintenance of several inflammatory responses recruiting activating neutrophils monocytes1. Here we provide longitudinal analysis immune responses, including phenotypic analyses cells assessments soluble factors that are present blood...
Inhibitory receptors for MHC class I molecules increase the threshold of lymphocyte activation. Natural Killer (NK) cells express a large number such inhibitory receptors, including human killer Ig-like (KIR). However, activating members KIR family have poorly defined ligands and functions. Here we describe use tetramer reagents as probes to detect their ligands. Infection with Epstein–Barr virus leads expression detectable ligand receptor KIR2DS1. In this case, KIR2DS1 interacts...
Abstract NK cells express cell surface receptors for MHC class I proteins (KIR). Engagement of these inhibits cytotoxic programs. KIR can be expressed on T cells, and their engagement also results in inhibition effector functions initiated by the CD3/TCR complex. While human genes belong to Ig gene superfamily, mouse a family dimeric lectins. Despite distinct evolutionary origins, we show here that both HLA-Cw3-specific p58.183 H-2D d/k-specific Ly49A recruit same protein tyrosine...
Subsets of T and natural killer (NK) lymphocytes express the CD94-NKG2A heterodimer, a receptor for major histocompatibility complex class I molecules. We show here that engagement heterodimer inhibits both antigen-driven tumor necrosis factor (TNF) release cytotoxicity on melanoma-specific human cell clones. Similarly, CD16-mediated NK is extinguished by cross-linking heterodimer. Combining in vivo vitro analysis, we report I/VxYxxL immunoreceptor tyrosine-based inhibition motifs (ITIM)...
An emerging family of cell surface inhibitory receptors is characterized by the presence intracytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIM). These ITIM-bearing receptors, which are typically paired with activating isoforms, associate Src homology domain 2-containing phosphatases following ITIM tyrosine phosphorylation. Two categories recruited receptors: protein-tyrosine phosphatases, SHP-1 and SHP-2, polyphosphate inositol 5-phosphatase, SHIP. The dynamic equilibrium B...
Natural Killer Controls Cytolytic natural killer (NK) cells participate in both antimicrobial and antitumor immunity. Their responsiveness is tuned through signals received a variety of inhibitory activating receptors expressed on their cell surface. Narni-Mancinelli et al. (p. 344 ) now show that signaling the receptor NKp46 paradoxically keeps NK responses check. from mice with disrupted expression were hyperresponsive to stimulation better protected against viral infection. responses,...
Ikaros/IKZF1 is an essential transcription factor expressed throughout hematopoiesis. IKZF1 implicated in lymphocyte and myeloid differentiation negative regulation of cell proliferation. In humans, somatic mutations have been linked to the development B acute lymphoblastic leukemia (ALL) children adults. Recently, heterozygous germline identified patients with a immune deficiency mimicking common variable immunodeficiency. These demonstrated incomplete penetrance led haploinsufficiency....
Statement of Purpose. IgG4-related disease (IgG4-RD) is usually associated to an increase serum IgG4 levels. However other conditions have also been high Methods. All IgG subclasses analyses performed in our hospital over a one-year period were analyzed. When level 1.35 g/L, the patient's clinical observation was analyzed and both final diagnosis reason leading analysis recorded. Only polyclonal increases considered. Summary Results. On 646 subclass performed, 59 patients had g/L. The very...
Natural killer cell activity and NKp30/B7-H6 interaction are prognostic biomarkers in high-risk neuroblastoma patients.
Inborn errors of DNA repair or replication underlie a variety clinical phenotypes. We studied 5 patients from 4 kindreds, all whom displayed intrauterine growth retardation, chronic neutropenia, and NK cell deficiency. Four the also had postnatal retardation. The association neutropenia deficiency, which is unusual among primary immunodeficiencies bone marrow failures, was due to blockade in mildly symptomatic. discovered compound heterozygous rare mutations Go-Ichi-Ni-San (GINS) complex...
Natural killer (NK) cells are innate cytotoxic lymphoid (ILCs) involved in the killing of infected and tumor cells. Among human mouse NK from spleen blood, we previously identified by single-cell RNA sequencing (scRNAseq) two similar major subsets, NK1 NK2. Using same technology, report here identification, (scRNAseq), three cell subpopulations bone marrow. Pseudotime analysis a subset resident CD56bright cells, NK0 as precursor both circulating CD56dim NK1-like NK2-like marrow under...
Abstract Immunoreceptor tyrosine-based inhibition motifs (ITIMs) consist of a 6-amino acid stretch (ILV)xYxx(LV). ITIMs are expressed in the intracytoplasmic domain transmembrane receptors, which extinguish cell activation induced by receptors bearing immunoreceptor motifs. The recent identification new members ITIM-bearing receptor family (such as signal-regulatory proteins) extends ITIM concept to negative regulation growth factor- and oncogene-induced nonhemopoietic cells. In addition, it...
Natural killer cell and T subsets express at their surface a repertoire of receptors for MHC class I molecules, the natural (NKRs). NKRs are characterized by existence inhibitory activating isoforms, which encoded highly homologous but separate genes present in same locus. Inhibitory isoforms an intracytoplasmic immunoreceptor tyrosine-based inhibition motif, whereas lack any motif harbor charged amino acid residue transmembrane domain. We previously KARAP (killer receptor-associated...
Abstract A novel family of inhibitory co‐receptors has been recently defined according to the presence in their intracytoplasmic domain immunoreceptor tyrosine‐based inhibition motifs (ITIM). In particular, this includes a low‐affinity receptor for IgG, FcγRIIB, which is widely expressed on hematopoietic cells, as well killer cell receptors (KIR) major histocompatibility complex (MHC) class I proteins, both T and natural (NK) lymphocytes. FcγRIIB KIR function depends upon tyrosine...
Objectives— During inflammation, cell adhesion molecules are modulated or redistributed for leukocyte transmigration. Among at the interendothelial junction, CD146 is involved in cell–cell cohesion and permeability, but its role monocyte transmigration unknown. Methods Results— TNF enhanced expression junction apical membrane of human umbilical veins endothelial cells (HUVECs) through synthesis intracellular store redistribution. In addition, increased release a soluble form (sCD146)...
Infection with SARS-COV-2 is the cause of COVID-19 and has generated an unprecedented health crisis worldwide. While most patients experience mild symptoms, around 20% develop severe disease, characterized by pneumonia in worst cases acute respiratory distress syndrome (ARDS).1 The analysis understanding immune responses arising course infection may help to propose therapeutic solutions. Due crucial role Natural Killer (NK) cells antiviral responses,2 we analyzed NK blood from a cohort 82...