A5032944800- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Eosinophilic Esophagitis
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Zebrafish Biomedical Research Applications
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Blood groups and transfusion
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Growth Hormone and Insulin-like Growth Factors
- Hematopoietic Stem Cell Transplantation
- Vitamin C and Antioxidants Research
- Cancer Immunotherapy and Biomarkers
- Platelet Disorders and Treatments
- RNA and protein synthesis mechanisms
- Diabetes and associated disorders
- bioluminescence and chemiluminescence research
Université Paris Cité
2015-2024
Institut Pasteur
2015-2024
Inserm
2015-2024
Université Paris-Saclay
2022
Délégation Paris 7
2002-2020
Sorbonne Paris Cité
2011-2020
Délégation Paris 5
2019
Centre National de la Recherche Scientifique
1982-2004
Institut Gustave Roussy
2004
Ontario Institute for Cancer Research
1998-2002
Inflammation triggers the differentiation of Ly6Chi monocytes into microbicidal macrophages or monocyte-derived dendritic cells (moDCs). Yet, it is unclear whether environmental inflammatory cues control polarization toward each these fates specialized monocyte progenitor subsets exist before inflammation. Here, we have shown that naive are phenotypically heterogeneous and contain an NR4A1- Flt3L-independent, CCR2-dependent, Flt3+CD11c−MHCII+PU.1hi subset. This subset acted as a precursor...
Tissue repair processes maintain proper organ function following mechanical or infection-related damage. In addition to antibacterial properties, mucosal associated invariant T (MAIT) cells express a tissue transcriptomic program and promote skin wound healing when expanded. Herein, we use human-like mouse model of full-thickness excision assess the underlying mechanisms MAIT cell function. Single-cell RNA sequencing analysis suggested that already at steady state. Following excision,...
Little is known about hematopoietic stem cell (HSC) development from mesoderm. To gain more information on the intraembryonic HSC site of origin, we purified multipotent progenitors aorta–gonads–mesonephros (AGM) mice. This population, expressing c-Kit, AA4.1, CD31, and CD41, but not Flk1, mainly negative for CD45, proved capable long-term reconstitution in sublethally irradiated Rag2γc -/- recipients. We assigned expression GATA-2, GATA-3, lmo2 to AGM-HSC, whereas erythromyeloid express...
Background Resident memory T lymphocytes (T RM ) are located in tissues and play an important role immunosurveillance against tumors. The presence of prior to treatment or their induction is associated the response anti-Programmed cell death protein 1 (PD-1)/Programmed death-ligand (PD-L1) immunotherapy efficacy cancer vaccines. Previous work by our group others has shown that intranasal route vaccination allows more efficient these cells head neck lung mucosa, resulting better tumor...
T and innate lymphoid cells (ILCs) share some aspects of their developmental programs. However, although Notch signaling is strictly required for cell development, it dispensable fetal ILC development. Constitutive activation signaling, at the common progenitor stage, drives development abrogates by preventing Id2 expression. By combining single-cell transcriptomics clonal culture strategies, we characterize two heterogeneous α4β7-expressing compartments. αLP1 (Flt3(+)) still retains...
The balance in the proportions of innate lymphoid cell subsets depends on signaling by receptor Notch2.
Primary myelofibrosis is a myeloproliferative neoplasm that precursor to myeloid leukemia. Dysmegakaryopoiesis and extramedullary hematopoiesis characterize primary myelofibrosis, which also associated with bone marrow stromal alterations marked by fibrosis, neoangiogenesis, osteomyelosclerosis. In particular, contributions from mesenchymal cells (MSC) have been suggested mouse studies, but evidence in humans remains lacking. this study, we show MSCs patients exhibit unique molecular...
Highlights•LTi progenitors originate from early embryonic hemogenic endothelium, excluding yolk sac•The fetal liver harbors proliferating LTi precursor populations•LTi maturation occurs in the periphery into two LTi4 populations•Embryonic are replaced by HSC-derived cells adultsSummaryDuring embryogenesis, lymphoid tissue inducer (LTi) essential for lymph node organogenesis. These part of innate cell (ILC) family. Although their earliest hematopoietic origin is unclear, other immune have...
Innate lymphoid cells are present at mucosal sites and represent the first immune barrier against infections, but what contributes to their circulation homing is still unclear. Using Rag2 −/− Cxcr6 Gfp /+ reporter mice, we assessed expression role of CXCR6 in ILC precursors progeny. We identify expressing bone marrow characterize significant increase CXCR6‐deficient mice steady state, indicating partial retention after ablation. Circulation was also impaired during embryonic life as fetal...
Abstract RANK and its ligand RANKL play important roles in the development regulation of immune system. We show that mice transgenic for Rank hair follicles display massive postnatal growth skin-draining lymph nodes. The proportions hematopoietic nonhematopoietic stromal cells their organization are maintained, with exception an increase B cell follicles. not activated respond to immunization by foreign Ag adjuvant. demonstrate soluble is overproduced from neutralization normalizes node...
The role of the fetal spleen in hematopoeisis remains largely unknown. In this particular environment, we show that hematopoietic stem cells do not proliferate, but they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes derive from committed cell precursors are likely to have immigrated liver. We developed stromal lines unique their capacity expand myeloid precursors, resulting large numbers These secrete high levels anti-inflammatory molecules. By...
Abstract For the formation of lymph nodes and Peyer’s patches, lymphoid tissue inducer (LTi) cells are crucial in triggering stromal to recruit retain hematopoietic cells. Although LTi have been observed fetal spleen, not much is known about spleen development role this process. Here, we show that collect a periarteriolar manner at periphery white pulp anlagen. Expression homeostatic chemokines can be detected endothelial cells, suggesting attracted by these chemokines. As lymphotoxin...
The Notch pathway is one of the canonical signaling pathways implicated in development various solid tumors. During carcinogenesis, disregulation induces tumor expression receptor ligands participating to escape immune surveillance. conditions both and functional regulation lymphoid subsets. Its importance on T cell subset polarization has been documented contrary its action innate cells (ILC). We aim analyze effect type 1 ILC functions after disruption RBPJk-dependent cascade. Indeed,...