A5102911802- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- RNA Interference and Gene Delivery
- interferon and immune responses
- Phagocytosis and Immune Regulation
- Galectins and Cancer Biology
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Force Microscopy Techniques and Applications
- Macrophage Migration Inhibitory Factor
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
Institut Curie
2015-2024
Inserm
2015-2024
Immunité et Cancer
2016-2024
Université Paris Sciences et Lettres
2016-2024
Institut Pasteur
2001-2021
Harvard University
1999-2002
Inflammation triggers the differentiation of Ly6Chi monocytes into microbicidal macrophages or monocyte-derived dendritic cells (moDCs). Yet, it is unclear whether environmental inflammatory cues control polarization toward each these fates specialized monocyte progenitor subsets exist before inflammation. Here, we have shown that naive are phenotypically heterogeneous and contain an NR4A1- Flt3L-independent, CCR2-dependent, Flt3+CD11c−MHCII+PU.1hi subset. This subset acted as a precursor...
Immune cells experience large cell shape changes during environmental patrolling because of the physical constraints that they encounter while migrating through tissues. These can adapt to such deformation events using dedicated shape-sensing pathways. However, how sensing affects immune function is mostly unknown. Here, we identify a mechanism increases expression chemokine receptor CCR7 and guides dendritic migration from peripheral tissues lymph nodes at steady state. This relies on lipid...
Before a class II molecule can be loaded with antigenic material and reach the surface to engage CD4+ T cells, its chaperone, II-associated invariant chain (Ii), is degraded in stepwise fashion by proteases endocytic compartments. We have dissected role of cathepsin S (CatS) trafficking maturation molecules combining use dendritic cells (DC) from CatS−/− mice new active site–directed probe for direct visualization CatS. Our data demonstrate that CatS along entire route, cleavage lysosomal...
Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation active proteases into phagosome macrophage cell line J774 indicates maturation involves progressive fusion with early and late endocytic compartments. In contrast, biogenesis bone marrow–derived dendritic cells (DCs) macrophages...
Abstract Cell polarity is required for the functional specialization of many cell types including lymphocytes. A hallmark reorientation centrosome that allows repositioning organelles and vesicles in an asymmetric fashion. The mechanisms underlying polarization are not fully understood. Here we found resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which needed tethering to nucleus via LINC complex. Upon lymphocyte activation, partially depleted from as a result...
Significance To mount an efficient immune response, T cells need to recognize foreign antigen become activated, proliferate, and acquire their effector functions. We show that can adopt two behaviors depending on the strength of stimulation. Upon suboptimal stimulation, a scanning behavior promotes local exploration microenvironment. Stronger stimulation triggers specific mechanisms induce complete T-cell arrest. propose these distinct may represent optimal strategy for find strongest antigenic
Abstract An important channel of cell-to-cell communication is direct contact. The immune synapse a paradigmatic example such type interaction: it forms upon engagement antigen receptors in lymphocytes by antigen-presenting cells and allows the local exchange molecules information. Although mechanics has been shown to play an role this process, how forces organize impact on function unknown. We find that mechanical are spatio-temporally patterned at synapse: global pulsatile myosin II-driven...
Dendritic cells (DCs) patrol tissues and transport antigens to lymph nodes initiate adaptive immune responses. Within tissues, DCs constitute a complex cell population composed of distinct subsets that can exhibit different activation states functions. How tissue-specific cues orchestrate DC diversification remains elusive. Here, we show the small intestine included two pools cDC2s originating from common pre-DC precursors: (1) lamina propria (LP) CD103
The immune synapse (IS) forms as dendritic cells (DCs) and T interact in lymph nodes during initiation of adaptive immunity. Factors that contribute to the formation maintenance IS stability function have been mostly studied cells, whereas little is known about events occurring DCs. Here, we show DCs activated by Toll-like receptor (TLR) agonists reorient microtubule-organizing center (MTOC) toward interacting cell antigen-specific through a mechanism depends on Rho GTPase Cdc42. IL-12,...
B-cell receptor (BCR) engagement with surface-tethered antigens leads to the formation of an immune synapse, which facilitates antigen uptake for presentation T-lymphocytes. Antigen internalization and processing rely on early dynein-dependent transport BCR-antigen microclusters synapse center, as well later polarization microtubule-organizing center (MTOC). MTOC repositioning allows release proteases delivery MHC class II molecules at synapse. Whether how these events are coordinated have...
Immune synapse formation is a key step for lymphocyte activation. In B lymphocytes, the immune controls production of high-affinity antibodies, thereby defining efficiency humoral responses. While roles played by both actin and microtubule cytoskeletons in function have become increasingly clear, how different events involved are coordinated space time actin–microtubule interactions not understood. Using microfluidic pairing device, we studied with unprecedented resolution dynamics various...