Sylvain Baulande
A5077251700- Cancer Genomics and Diagnostics
- Neuroblastoma Research and Treatments
- Immune cells in cancer
- Ocular Oncology and Treatments
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Sarcoma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- RNA Research and Splicing
- Glioma Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
Institut Curie
2016-2025
Université Paris Sciences et Lettres
2017-2025
Inserm
2015-2024
Goethe University Frankfurt
2024
Institut Mondor de Recherche Biomédicale
2024
Macquarie University
2024
Genopole (France)
2007-2021
Centre National pour la Recherche Scientifique et Technique (CNRST)
2021
Université Paris Cité
2019
Délégation Paris 5
2019
A subset of cancer-associated fibroblasts (FAP+/CAF-S1) mediates immunosuppression in breast cancers, but its heterogeneity and impact on immunotherapy response remain unknown. Here, we identify 8 CAF-S1 clusters by analyzing more than 19,000 single from cancer. We validate the five most abundant flow cytometry silico analyses other cancer types, highlighting their relevance. Myofibroblasts 0 3, characterized extracellular matrix proteins TGFβ signaling, respectively, are indicative primary...
Recent clinical results support the use of new immune checkpoint blockers (ICB), such as anti-PD-1 (e.g. nivolumab and pembrolizumab) anti-PD-L1 antibodies. Radiological evaluation ICB efficacy during therapy is challenging due to tumor infiltration. Changes circulating DNA (ctDNA) levels could be a promising tool for very accurate monitoring treatment efficacy, but data are lacking with ICB.This prospective pilot study was conducted in patients nonsmall cell lung cancer, uveal melanoma, or...
Abstract Purpose: One of the main limitations to anticancer radiotherapy lies in irreversible damage healthy tissues located within radiation field. “FLASH” irradiation at very high dose-rate is a new treatment modality that has been reported specifically spare normal tissue from late radiation-induced toxicity animal models and therefore could be promising strategy reduce toxicity. Experimental Design: Lung responses FLASH were investigated by qPCR, single-cell RNA sequencing (sc-RNA-Seq),...
Presentation of exogenous antigens on MHC-I molecules, termed cross-presentation, is essential for cytotoxic CD8+ T cell responses. In mice, dendritic cells (DCs) that arise from monocytes (mo-DCs) during inflammation have a key function in these responses by cross-presenting locally peripheral tissues. Whether human naturally-occurring mo-DCs can cross-present unknown. Here, we use and macrophages directly purified ascites to address this question. Single-cell RNA-seq data show CD1c+ DCs...
Abstract Tumor-associated macrophages (TAM) play a detrimental role in triple-negative breast cancer (TNBC). In-depth analysis of TAM characteristics and interactions with stromal cells, such as cancer-associated fibroblast (CAF), could provide important biological therapeutic insights. Here we identify at the single-cell level monocyte-derived STAB1+TREM2high lipid-associated macrophage (LAM) subpopulation immune suppressive capacities that is expanded patients resistant to checkpoint...
Abstract Inflammation is a complex physiological process triggered in response to harmful stimuli 1 . It involves cells of the immune system capable clearing sources injury and damaged tissues. Excessive inflammation can occur as result infection hallmark several diseases 2–4 The molecular bases underlying inflammatory responses are not fully understood. Here we show that cell surface glycoprotein CD44, which marks acquisition distinct phenotypes context development, immunity cancer...
CD8 + T cells in lung tumors differentiate through convergent pathways to an expanded and actively cycling terminal state.
Oncogenesis often implicates epigenetic alterations, including derepression of transposable elements (TEs) and defects in alternative splicing. Here, we explore the possibility that noncanonical splice junctions between exons TEs represent a source tumor-specific antigens. We show mouse normal tissues tumor cell lines express wide but distinct ranges mRNA TEs, some which are specific. Immunopeptidome analyses identified peptides derived from exon-TE splicing associated to MHC-I molecules....
Abstract Noradrenergic and mesenchymal identities have been characterized in neuroblastoma cell lines according to their epigenetic landscapes core regulatory circuitries. However, relationship relative contribution patient tumors remain poorly defined. We now document spontaneous reversible plasticity between the two identities, associated with reprogramming, several models. Interestingly, xenografts cells from each identity eventually harbor a noradrenergic phenotype suggesting that...
Abstract Although heterogeneity of FAP+ Cancer-Associated Fibroblasts (CAF) has been described in breast cancer, their plasticity and spatial distribution remain poorly understood. Here, we analyze trajectory inference, deconvolute transcriptomics at single-cell level perform functional assays to generate a high-resolution integrated map cancer (BC), with focus on inflammatory myofibroblastic (iCAF/myCAF) CAF clusters. We identify 10 spatially-organized CAF-related cellular niches, called...
We have used a mRNA differential display technique to identify new genes involved in the reprogramming of gene expression during adipose conversion mouse 3T3 preadipocyte cell lines. report here on identification and cloning novel adipose-specific cDNA encoding predicted membrane protein 413 amino acids. The level corresponding 3.2-kilobase is tremendously increased 3T3-L1 3T3-F442A differentiation into adipocytes. A single, very abundant transcript also found inguinal epididymal white...
Purpose: Neuroblastoma displays important clinical and genetic heterogeneity, with emergence of new mutations at tumor progression.Experimental Design: To study clonal evolution during treatment follow-up, an innovative method based on circulating cell-free DNA (cfDNA) analysis by whole-exome sequencing (WES) paired target was realized in sequential liquid biopsy samples 19 neuroblastoma patients.Results: WES the primary cfDNA diagnosis showed overlap single-nucleotide variants (SNV) copy...
EWSR1-FLI1, the chimeric oncogene specific for Ewing sarcoma (EwS), induces a cascade of signaling events leading to cell transformation. However, it remains elusive how genetically homogeneous EwS cells can drive heterogeneity transcriptional programs. Here, we combine independent component analysis single-cell RNA sequencing data from diverse types and model systems with time-resolved mapping EWSR1-FLI1 binding sites open chromatin regions characterize dynamic cellular processes associated...
A significant proportion of patients with oestrogen receptor (ER) positive breast cancers (BC) develop resistance to endocrine treatments (ET) and relapse metastatic disease. Here we perform whole exome sequencing gene expression analysis matched primary tumours bone metastasis-derived patient-derived xenografts (PDX). Transcriptomic analyses reveal enrichment the G2/M checkpoint up-regulation Polo-like kinase 1 (PLK1) in PDX. PLK1 inhibition results tumour shrinkage highly proliferating...
The colon is primarily responsible for absorbing fluids. It contains a large number of microorganisms including fungi, which are enriched in its distal segment. colonic mucosa must therefore tightly regulate fluid influx to control absorption fungal metabolites, can be toxic epithelial cells and lead barrier dysfunction. How this achieved remains unknown. Here, we describe mechanism by the innate immune system allows rapid quality check absorbed fluids avoid intoxication colonocytes. This...
Abstract Retinoblastoma is the most frequent intraocular malignancy in children, originating from a maturing cone precursor developing retina. Little known on molecular basis underlying biological and clinical behavior of this cancer. Here, using multi-omics data, we demonstrate existence two retinoblastoma subtypes. Subtype 1, earlier onset, includes heritable forms. It harbors few genetic alterations other than initiating RB1 inactivation corresponds to differentiated tumors expressing...
In neuroblastoma (NB), the ALK receptor tyrosine kinase can be constitutively activated through activating point mutations or genomic amplification. We studied genetic alterations in high-risk (HR) patients on HR-NBL1/SIOPEN trial to determine their frequency, correlation with clinical parameters, and prognostic impact.Diagnostic tumor samples were available from 1,092 amplification status (n = 330), mutational profile 191), both 571).Genomic (ALKa) was detected 4.5% of cases (41 out 901),...