A5058069900- Cancer Genomics and Diagnostics
- Neuroblastoma Research and Treatments
- Genomic variations and chromosomal abnormalities
- Gene expression and cancer classification
- Blood groups and transfusion
- Breast Cancer Treatment Studies
- Erythrocyte Function and Pathophysiology
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Glioma Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Cancer, Hypoxia, and Metabolism
- Protein Tyrosine Phosphatases
- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- Ocular Oncology and Treatments
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Cancer therapeutics and mechanisms
- CRISPR and Genetic Engineering
- BRCA gene mutations in cancer
Institut Curie
2016-2025
Université Paris Sciences et Lettres
2016-2025
Inserm
2014-2023
KU Leuven
2019
Agendia (Netherlands)
2019
Immunité et Cancer
2019
Directorate-General Joint Research Centre
2013
Institut National de Recherche en Santé Publique
2007
Université Sorbonne Nouvelle
2006
Institut National de la Transfusion Sanguine
1993-2002
Abstract Noradrenergic and mesenchymal identities have been characterized in neuroblastoma cell lines according to their epigenetic landscapes core regulatory circuitries. However, relationship relative contribution patient tumors remain poorly defined. We now document spontaneous reversible plasticity between the two identities, associated with reprogramming, several models. Interestingly, xenografts cells from each identity eventually harbor a noradrenergic phenotype suggesting that...
Abstract Introduction Typical medullary breast carcinoma (MBC) has recently been recognized to be part of the basal-like spectrum, a feature in agreement with high rate TP53 mutations previously reported MBCs. The present study was therefore designed identify phenotypic and genetic alterations that distinguish MBCs from carcinomas (BLC). Methods Expression levels estrogen receptor (ER), progesterone (PR), ERBB2, TP53, cytokeratins (KRTs) 5/6, 14, 8/18, epidermal growth factor KIT, as well...
To gain insight into genomic and transcriptomic subtypes of ductal carcinomas in situ the breast (DCIS).We did a combined phenotypic analysis series 57 DCIS integrated with gene expression profile for 26 cases.Thirty-two exhibited luminal phenotype; 21 were ERBB2 positive, 4 ERBB2/estrogen receptor (ER) negative 1 harboring bona fide basal-like phenotype. Based on CGH analysis, types identified this 1q gain/16q loss combination observed 3 DCIS, mixed amplifier pattern including all ERBB2, 12...
Circulating tumor DNA (ctDNA) is a new circulating biomarker which might be used as prognostic in way similar to cells (CTCs). Here, we the high prevalence of TP53 mutations triple negative breast cancer (TNBC) compare ctDNA and CTC detection rates value metastatic TNBC patients. Forty patients were enrolled before starting line treatment. characterized archived tissues plasma using two next generation sequencing (NGS) platforms parallel. Archived tissue was sequenced successfully for 31/40...
EWSR1-FLI1, the chimeric oncogene specific for Ewing sarcoma (EwS), induces a cascade of signaling events leading to cell transformation. However, it remains elusive how genetically homogeneous EwS cells can drive heterogeneity transcriptional programs. Here, we combine independent component analysis single-cell RNA sequencing data from diverse types and model systems with time-resolved mapping EWSR1-FLI1 binding sites open chromatin regions characterize dynamic cellular processes associated...
Metastatic uveal melanoma is a deadly disease with no proven standard of care. Here we present metastatic patient an exceptional high sensitivity to PD-1 inhibitor associated outlier CpG>TpG mutation burden, MBD4 germline deleterious mutation, and somatic inactivation in the tumor. We identify additional tumors The Cancer Genome Atlas (TCGA) cohorts similar hypermutator profiles patients carrying mutations loss heterozygosity. This MBD4-related phenotype may explain unexpected responses...
CDK12 is a recurrently mutated gene in serous ovarian carcinoma, whose downregulation associated with impaired expression of DNA damage repair genes and subsequent hypersensitivity to DNA-damaging agents PARP1/2 inhibitors. In this study, we investigated the genomic landscape inactivation patients carcinoma. We show that loss was consistently particular instability pattern characterized by hundreds tandem duplications up 10 megabases (Mb) size. Tandem were bimodal (∼0.3 ∼3 Mb) size...
Abstract Resistance to endocrine treatments and CDK4/6 inhibitors is considered a near-inevitability in most patients with estrogen receptor positive breast cancers (ER + BC). By genomic metabolomics analyses of patients’ tumours, metastasis-derived patient-derived xenografts (PDX) isogenic cell lines we demonstrate that fraction metastatic ER BC highly reliant on oxidative phosphorylation (OXPHOS). Treatment by the OXPHOS inhibitor IACS-010759 strongly inhibits tumour growth multiple...
Abstract Motivation: Microarray-based CGH (Comparative Genomic Hybridization), transcriptome arrays and other large-scale genomic technologies are now routinely used to generate a vast amount of profiles. Exploratory analysis this data is crucial in helping understand the help form biological hypotheses. This step requires visualization meaningful way visualize results perform first level analyses. Results: We have developed graphical user interface for molecular It currently use at Institut...
Abstract The pathological classification of gliomas constitutes a critical step the clinical management patients, yet it is frequently challenging. To assess relationship between genetic abnormalities and clinicopathological characteristics, we have performed analysis series gliomas. A total 112 were analyzed by comparative genomic hybridization on BAC array with 1 megabase resolution. Altered regions identified correlation enabled to retrieve significant associations exclusions. Whole...
Abstract The 8p11-12 chromosome region is one of the regions most frequently amplified in breast carcinoma (10–15% cases). Several genes within this have been identified as candidate oncogenes, they are both and overexpressed. However, very few studies explored role these cell transformation, with aim identifying valuable therapeutic targets. An analysis comparative genomic hybridization array expression profiling data for a series 152 ductal carcinomas 21 lines five (LSM1, BAG4, DDHD2,...
Abstract Predicting evolution of small node‐negative breast carcinoma is a real challenge in clinical practice. The aim this study was to search whether qualitative or quantitative DNA changes may help predict metastasis carcinoma. Small invasive ductal carcinomas without axillary lymph node involvement (T1T2N0) from 168 patients with either good (111 no event at 5 years after diagnosis) poor (57 early metastasis) outcome were analyzed comparative genomic hybridization (CGH) array. A CGH...
Background High-risk neuroblastoma is a pediatric cancer with still dismal prognosis, despite multimodal and intensive therapies. Tumor microenvironment represents key component of the tumor ecosystem complexity which has to be accurately understood define selective targeting opportunities, including immune-based Methods We combined various approaches single-cell transcriptomics dissect both transgenic mouse model cohort 10 biopsies from patients, either at diagnosis or relapse. Features...
Abstract Identification among breast tumors of those arising in a hereditary BRCA1 context remains medical challenge. Abnormalities X chromosome copy number and the epigenetic stability inactive (Xi) have been proposed to characterize tumors. In particular, it has that loss function can lead inactive–specific transcript (XIST) RNA association with Xi. However, few studies addressed this issue sufficiently large series primary Here we assess X-chromosome status using single-cell (RNA DNA...
Pure invasive micropapillary carcinoma (IMPC) is a special type of breast characterised by clusters cells presenting polarity abnormalities. The biological alterations underlying this pattern remain unknown. Pangenomic analysis (n = 39), TP53 43) and PIK3CA 41) sequencing in series IMPCs were performed. A subset cases was also analysed with whole-exome 4) RNA 6). Copy number variation profiles compared those oestrogen receptors grade-matched ductal carcinomas (IDCs) no type. Unsupervised...