A5076400024- Ocular Oncology and Treatments
- Cutaneous Melanoma Detection and Management
- Nonmelanoma Skin Cancer Studies
- Cancer-related Molecular Pathways
- Retinal Development and Disorders
- Cancer Genomics and Diagnostics
- Ocular Infections and Treatments
- Glaucoma and retinal disorders
- Corneal Surgery and Treatments
- Head and Neck Surgical Oncology
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Cancer and Skin Lesions
- Retinal Diseases and Treatments
- Glioma Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Retinal and Macular Surgery
- Vascular Tumors and Angiosarcomas
- Brain Metastases and Treatment
- Epigenetics and DNA Methylation
- Multiple Myeloma Research and Treatments
- interferon and immune responses
- Ear and Head Tumors
- Ocular Diseases and Behçet’s Syndrome
Institut Curie
2014-2023
Société Française de Cardiologie
2023
Université Paris Sciences et Lettres
2018-2020
University of Lubumbashi
2018
University of Kinshasa
2018
Institut Claudius Regaud
2018
Délégation Paris 6
2005-2014
Fondation Ophtalmologique Adolphe de Rothschild
2014
La Ligue Contre le Cancer
2013
Inserm
2010-2013
Uveal melanoma, the most common eye malignancy, causes severe visual morbidity and is fatal in approximately 50% of patients. Primary uveal melanoma can be cured by surgery or radiotherapy, but metastatic disease treatment refractory. To understand comprehensively genetics, we conducted single-nucleotide polymorphism arrays whole-genome sequencing on 12 primary melanomas. We observed only 2,000 predicted somatic variants per tumor low levels aneuploidy. did not observe an ultraviolet...
PurposeTo present the American Brachytherapy Society (ABS) guidelines for plaque brachytherapy of choroidal melanoma and retinoblastoma.Methods MaterialsAn international multicenter Ophthalmic Oncology Task Force (OOTF) was assembled to include 47 radiation oncologists, medical physicists, ophthalmic oncologists from 10 countries. The ABS-OOTF produced collaborative guidelines, based on their eye cancer–specific clinical experience knowledge literature. This work reviewed approved by ABS...
Microscopic extrascleral involvement and of optic nerve resection line are accepted risk factors for orbital and/or metastatic disease from retinoblastoma. Conversely, choroidal retrolaminar questionable factors. The aim this retrospective study was to define the histopathologic in patients treated by first-line enucleation.Histopathologic review 172 evaluable at Institut Curie between 1977 1990 determined degree (minimal or massive), scleral (intra- extrascleral), (prelaminar, with without...
Circulating tumor cells (CTCs) and circulating DNA (ctDNA) have been recently investigated in several cancer types, but their respective clinical significance remains to be determined. In our prospective study, we compared the detection rate prognostic value of these two biomarkers patients with metastatic uveal melanoma. GNAQ/GNA11 mutations were characterized archived tissue. Using a highly sensitive mutation‐specific bidirectional pyrophosphorolysis‐activated polymerization (bi‐PAP)...
Purpose To refine the anatomic classification and staging of ciliary body choroidal melanomas in TNM classification. Patients Methods Tumor largest basal diameter thickness 7,369 patients were analyzed based on registry data from five ocular oncology centers. T categories derived empirically by dividing into blocks representing 3- × 3-mm fractions. Blocks with similar survival grouped together so that no category comprised a large majority tumors, each was uniform survival, using randomly...
A high percentage of uveal melanoma patients develop metastatic tumors predominantly in the liver. We studied molecular profiles derived from gene expression microarrays and comparative genomic hybridization microarrays, to identify genes associated with metastasis this aggressive cancer. compared 28 melanomas who developed liver metastases within three years enucleation 35 without or more than 3 after enucleation. Protein tyrosine phosphatase type IV member (PTP4A3/PRL3), was identified as...
Uveal melanoma is the most common primary intraocular malignant tumor in adults and defined by a poor natural outcome, as 50% of patients die from metastases. The aim this study was to develop characterize panel human uveal xenografts transplanted into immunodeficient mice.Ninety specimens were grafted severe combined mice, 25 transplantable then established (28%). Relationship between graft clinical, biological, therapeutic features included investigated. Characterization 16 histology,...
<h3>Objective</h3> This study investigated the capacity of genetic analysis uveal melanoma samples to identify high-risk patients and discusses its clinical implications. <h3>Methods</h3> Patients with posterior were prospectively enrolled. Tumour derived from enucleated globe, fine-needle aspirates or endoresection. Chromosome 3 8 status was determined by array comparative genomic hybridisation (array-CGH). followed after treatment detect metastasis. <h3>Results</h3> Four groups classified...
Abstract Retinoblastoma is the most frequent intraocular malignancy in children, originating from a maturing cone precursor developing retina. Little known on molecular basis underlying biological and clinical behavior of this cancer. Here, using multi-omics data, we demonstrate existence two retinoblastoma subtypes. Subtype 1, earlier onset, includes heritable forms. It harbors few genetic alterations other than initiating RB1 inactivation corresponds to differentiated tumors expressing...
Constitutional mutations of the RB1 gene are associated with a predisposition to retinoblastoma. It is essential identify these provide appropriate genetic counseling in retinoblastoma patients, but this represents an extremely challenging task, as vast majority unique and spread over entire coding sequence. Since 2001, we have implemented testing on routine basis part clinical management As most screening techniques do not meet requirements for efficient testing, devised semi-automated...
Incurable metastases develop in approximately 50% of patients with uveal melanoma (UM). The purpose this study was to analyze genomic profiles a large series ocular tumors and liver design genome-based classifier for metastatic risk assessment.A 86 UM 66 were analyzed by using BAC CGH (comparative hybridization) microarray. A clustering performed, correlation the status sought among subset 71 minimum follow-up 24 months. chromosome 3 further examined tumors, disomy checked an SNP prognostic...
Abstract Background Carboplatin plays an important role in the conservative management of retinoblastoma, but is associated with risk ototoxicity these young children whose sensory prognosis may be also compromised by their loss vision. This retrospective study analyzed impact carboplatin on hearing context retinoblastoma. Methods Data for 175 treated at Institut Curie between 1994 and 2002 were analyzed. Results Median age diagnosis was 8 months (0–60). administered 3 days (200 mg/m 2 /day)...
Abstract Purpose: To develop a molecular tool to detect circulating tumor–derived DNA (ctDNA) in the plasma from patients with uveal melanoma as marker of tumor burden and monitor treatment efficacy. Experimental Design: A real-time PCR was developed on basis bidirectional pyrophosphorolysis-activated polymerization (bi-PAP) for quantification ctDNA using 3′blocked primer pairs specific 3 recurrent mutually exclusive mutations Gα subunits GNAQ GNA11. Results: Sensitivity specificity bi-PAP...
Purpose The objective of this prospective study was to assess overall survival and event-free in patients with intraocular unilateral retinoblastoma (Reese-Ellsworth group V) treated by primary enucleation or without adjuvant therapy depending on histopathologic risk factors. Patients Methods (n = 123) were divided into three groups the basis factors for extraocular relapse metastasis assessed centralized histologic examination enucleated eyes. Group 1 70) had minimal no choroidal...