A5042658725- Radiation Therapy and Dosimetry
- Effects of Radiation Exposure
- Advanced Radiotherapy Techniques
- Neonatal Respiratory Health Research
- Telomeres, Telomerase, and Senescence
- Single-cell and spatial transcriptomics
- Cerebrovascular and genetic disorders
- Radiation Effects and Dosimetry
- Moyamoya disease diagnosis and treatment
- Molecular Biology Techniques and Applications
- Radiation Detection and Scintillator Technologies
- Tissue Engineering and Regenerative Medicine
- Medical Imaging and Pathology Studies
- DNA Repair Mechanisms
- Cell Adhesion Molecules Research
- Frailty in Older Adults
- Long-Term Effects of COVID-19
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cell Image Analysis Techniques
- Air Quality and Health Impacts
- PARP inhibition in cancer therapy
- Metalloenzymes and iron-sulfur proteins
- Machine Learning in Materials Science
- Digestive system and related health
- Environmental Monitoring and Data Management
Inserm
2015-2025
Institut Curie
2015-2025
Université Paris Sciences et Lettres
2017-2025
Centre National de la Recherche Scientifique
2015-2025
Université Paris-Saclay
2017-2025
Institut des Hautes Études Scientifiques
2022
Université Paris-Sud
2015-2017
Université Paris Cité
2008-2014
KU Leuven
2014
Délégation Paris 7
2008-2012
Pulsed, ultrahigh dose-rate irradiation is safer than standard radiotherapy for the treatment of lung cancer and selectively spares normal tissue.
Abstract Purpose: One of the main limitations to anticancer radiotherapy lies in irreversible damage healthy tissues located within radiation field. “FLASH” irradiation at very high dose-rate is a new treatment modality that has been reported specifically spare normal tissue from late radiation-induced toxicity animal models and therefore could be promising strategy reduce toxicity. Experimental Design: Lung responses FLASH were investigated by qPCR, single-cell RNA sequencing (sc-RNA-Seq),...
UHDpulse – Metrology for advanced radiotherapy using particle beams with ultra-high pulse dose rates is a recently started European Joint Research Project the aim to develop and improve dosimetry standards FLASH radiotherapy, very high energy electron (VHEE) laser-driven medical accelerators. This paper gives short overview about current state of developments electrons protons, as well particles related challenges in due rate during radiation pulses. We summarize objectives plans project...
Abstract Radiation Induced Lung Injury (RILI) is one of the main limiting factors thorax irradiation, which can induce acute pneumonitis as well pulmonary fibrosis, latter being a life-threatening condition. The order cellular and molecular events in progression towards fibrosis key to physiopathogenesis disease, yet their coordination space time remains largely unexplored. Here, we present an interactive murine single cell atlas lung response generated from C57BL6/J female mice. This tool...
Abstract Lung diseases develop when telomeres shorten beyond a critical point. We constructed mouse model in which the catalytic subunit of telomerase (mTert), or its catalytically inactive form (mTert CI ), is expressed from p21 Cdkn1a locus. Expression either TERT reduces global levels lungs aged mice, highlighting non-canonical function. However, only accumulation very short telomeres, oxidative damage, endothelial cell (ECs) senescence and senile emphysema mice. Single-cell analysis lung...
Abstract Recent progress in image-based spatial RNA profiling enables to spatially resolve tens hundreds of distinct species with high resolution. It presents new avenues for comprehending tissue organization. In this context, the ability assign detected transcripts individual cells is crucial downstream analyses, such as in-situ cell type calling. Yet, accurate segmentation can be challenging data, particular absence a high-quality membrane marker. To address issue, we introduce ComSeg,...
The most common causative diagnosis of hereditary small-vessel-disease the brain, CADASIL, is due to highly stereotyped mutations in NOTCH3 receptor. has 33 exons but all CADASIL occur within Epidermal Growth Factor-like Repeats encoded by 2–24, lead an odd number cysteine residues and are associated with GOM deposits abnormal protein accumulation. majority appear retain normal level signaling activity, while very few show reduced activity. Herein we identified a novel heterozygous missense...
Background and Purpose— Cerebral autosomal dominant arteriopathy with subcortical infarcts leucoencephalopathy, the most common heritable small vessel disease of brain, is caused by mutations in NOTCH3 receptor that stereotypically lead to age-dependent Notch3 ECD deposition vessels. loss function has been demonstrated for few mutations. However, whether this finding applies all a loss-of-function mechanism drives manifestations remain yet unknown. This study investigated vivo functionality...
Abstract Aim This study systematically investigates the radiobiological effects of Very High Energy Electrons (VHEE) generated by a laser-plasma accelerator (LPA), in comparison with Conventional Intermediate (CIEE) from conventional linear (LINAC). Using vitro, ex vivo , and models, we evaluate compare their potential toxicity on healthy tissues. Methods Cell survival, tissue response, developmental were assessed across three biological models. In vitro human fibroblasts (MRC5-hTERT) used...
Notch3 is critically important for the structure and myogenic response of distal arteries, particularly cerebral arteries. However, signaling pathways acting downstream remain largely unknown.Transcriptome analysis using tail arteries Notch3-null mice identified a core set 17 novel Notch3-regulated genes confirmed in or brain Postnatal deletion RBP-Jκ smooth muscle cells recapitulated structural, functional, molecular defects induced by deficiency. Transient vivo blockade Notch pathway with...
Abstract AsiDNA™, a cholesterol-coupled oligonucleotide mimicking double-stranded DNA breaks, was developed to sensitize tumour cells radio- and chemotherapy. This drug acts as decoy hijacking the damage response. Previous studies have demonstrated that standalone AsiDNA™ administration is well tolerated with no additional adverse effects when combined chemo- and/or radiotherapy. The lack of normal tissue complication encouraged further examination into role in cells. research demonstrates...
Breast cancer is a complex disease in which each patient could present several genetic alterations that are therapeutically relevant cancers. Here we explored the therapeutic benefit of combining PARP and mTOR inhibitors context DNA repair deficiency PI3K pathway activation. The combination everolimus olaparib was tested BRCA2-mutated patient-derived xenografts (PDX) carrying PI3K/AKT/mTOR pathway. An RPPA analysis different signalling pathways performed untreated treated xenografts....
To rapidly assess healthy tissue toxicities induced by new anti-cancer therapies (i.e., radiation alone or in combination with drugs), there is a critical need for relevant and easy-to-use models. Consistent the ethical desire to reduce use of animals medical research, we propose monitor lung toxicity using an ex vivo model. Briefly, freshly prepared organotypic slices from mice were irradiated, without being previously exposed chemotherapy, treatment was evaluated analysis cell division...
Lung cancers are highly resistant to radiotherapy, necessitating the use of high doses, which leads radiation toxicities such as pneumonitis and fibrosis. Caffeic Acid Phenethyl Ester (CAPE) has been suggested have anti-proliferative pro-apoptotic effects in tumour cells, while radioprotective anti-inflammatory anti-oxidant normal tissue. We investigated radiosensitizing CAPE lung cancer cell lines tissue vitro ex vivo, respectively.