A5012286745- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- Effects of Radiation Exposure
- Glioma Diagnosis and Treatment
- Medical Imaging Techniques and Applications
- DNA Repair Mechanisms
- Ocular Oncology and Treatments
- Radiation Detection and Scintillator Technologies
- CNS Lymphoma Diagnosis and Treatment
- Boron Compounds in Chemistry
- Carcinogens and Genotoxicity Assessment
- Lymphoma Diagnosis and Treatment
- PARP inhibition in cancer therapy
- Retinal Development and Disorders
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Cancer, Stress, Anesthesia, and Immune Response
- Lung Cancer Treatments and Mutations
- Nuclear Physics and Applications
- Retinal Diseases and Treatments
- Acute Myeloid Leukemia Research
- Molecular Biology Techniques and Applications
- Acute Lymphoblastic Leukemia research
- Radiation Effects and Dosimetry
- Cancer Immunotherapy and Biomarkers
Institut Curie
2014-2024
Université Paris Sciences et Lettres
2017-2024
Université Paris-Saclay
2010-2024
Inserm
2010-2024
Laboratoire de Recherche Translationnelle en Oncologie
2024
Université Paris-Sud
2017-2022
Polytechnic University of Turin
2018
Hôpital René Huguenin
2017
Centre de Radiothérapie Bayard
2017
Institut de Radioprotection et de Sûreté Nucléaire
2007-2010
Pulsed, ultrahigh dose-rate irradiation is safer than standard radiotherapy for the treatment of lung cancer and selectively spares normal tissue.
Objective— We hypothesized that adipose tissue may contain progenitors cells with cutaneous and angiogenic potential. Methods Results— Adipose tissue-derived stroma (ADSCs) were administrated to skin punched wounds of both nonirradiated irradiated mice (20 Gy, locally). At day14, ADSCs promoted dermal wound healing enhanced closure, viscolesticity, collagen secretion in mice. Interestingly, GFP-positive incorporated epidermal vivo expressed markers K5 K14. Cultured keratinocyte medium have...
Abstract Proton minibeam radiation therapy (pMBRT) is a novel strategy for minimizing normal tissue damage resulting from radiotherapy treatments. This partners the inherent advantages of protons with gain in preservation observed upon irradiation narrow, spatially fractionated beams. In this study, whole brains (excluding olfactory bulb) Fischer 344 rats (n = 16) were irradiated at Orsay Therapy Center. Half animals received standard proton irradiation, while other half pMBRT same average...
Proton minibeam radiation therapy (pMBRT) is a novel strategy which has already shown remarkable reduction in neurotoxicity as to compared with standard proton therapy. Here we report on the first evaluation of tumor control effectiveness glioma bearing rats highly spatially modulated beams. Whole brains (excluding olfactory bulb) Fischer 344 were irradiated. Four groups animals considered: group (RG2 rats), second RG2 tumor-bearing and third normal that received pMBRT (70 Gy peak dose one...
PurposeMinibeam radiation therapy (MBRT) is an innovative technique that uses a spatial dose modulation. The distribution consists of high doses (peaks) in the path minibeam and low (valleys). underlying biological mechanism associated with MBRT efficacy remains currently unclear thus we investigated potential role immune system after treatment MBRT.Methods MaterialsRats bearing orthotopic glioblastoma cell line were treated 1 fraction conventional (30 Gy) or same mean MBRT. Both...
Abstract Minibeam radiation therapy (MBRT) is an innovative synchrotron radiotherapy technique able to shift the normal tissue complication probability curves significantly higher doses. However, its exploration was hindered due limited and expensive beamtime at synchrotrons. The aim of this work develop a cost-effective equipment perform systematic radiobiological studies in view MBRT. Tumor control for various tumor entities will be addressable as well unravel distinct biological...
Abstract Radiotherapy (RT) is one of the most frequently used methods for cancer treatment. Despite remarkable advancements in RT techniquesthe treatment radioresistant tumours (i.e. high-grade gliomas) not yet satisfactory. Finding novel approaches less damaging normal tissues utmost importance. This would make it possible to increase dose applied tumours, resulting an improvement cure rate. Along this line, proton minibeam radiation therapy (pMBRT) a strategy that allows spatial modulation...
After large-scale accidental overexposure to ionizing radiation, a rapid triage of the exposed population can be performed by scoring dicentrics and ring chromosomes among 50 metaphases. This is but not accurate because sensitivity around 0.5 Gy. step, dose estimated 500 lengthy very between 0.1 0.2 To improve methodology, we propose use software for automatic dicentric that was tested on victims an accident in Dakar. Manual metaphases carried out, then manual metaphases, scoring. Comparison...
The medical options available to prevent or treat radiation-induced injury are scarce and developing effective countermeasures is still an open research field. In addition, more than half of cancer patients treated with radiation therapy, which displays a high antitumor efficacy but can cause, albeit rarely, disabling long-term toxicities including fibrosis. Progress has been made in the definition molecular pathways associated normal tissue toxicity that suggest potentially therapeutic...
(1) Background: Proton minibeam radiation therapy (pMBRT) is a new radiotherapy technique using spatially modulated narrow proton beams. pMBRT results in significantly reduced local tissue toxicity while maintaining or even increasing the tumor control efficacy as compared to conventional small animal experiments. In all experiments performed up date bearing animals, dose was delivered one single fraction. This first assessment on impact of temporal fractionation scheme response...
The study of radiation biology on laser-based accelerators is most interesting due to the unique irradiation conditions they can produce, in terms peak current and duration irradiation. In this paper we present implementation a beam transport system shape proton generated by laser-target interaction for vitro biological samples. A set four permanent magnet quadrupoles used focus beam, efficiently shaping spectrum providing large relatively uniform surface. Real time, absolutely calibrated,...
Therapeutic applications of synchrotron X-rays such as microbeam (MRT) and minibeam (MBRT) radiation therapy promise significant advantages over conventional clinical techniques for some diseases if successfully transferred to practice. Preclinical studies show clear evidence that a number normal tissues in animal models display tolerance much higher doses from MRT compared with radiotherapy. However, wide spread the parameters studied makes it difficult make any conclusions about associated...
Abstract Besides analyses of specific alternative splicing (AS) variants, little is known about AS regulatory pathways and programs involved in anticancer drug resistance. Doxorubicin widely used breast cancer chemotherapy. Here, we identified 1723 events 41 factors regulated a cell model acquired resistance to doxorubicin. An RNAi screen on the studied ZRANB2 SYF2, whose depletion partially reversed doxorubicin By RNA-seq resistant cells, found that controlled by SYF2 were enriched...
Abstract Background Radiation-induced neurocognitive dysfunction is a major adverse effect of brain radiation therapy and has specific relevance in pediatric oncology, where serious cognitive deficits have been reported survivors tumors. Moreover, many patients receive proton under general anesthesia or sedation to guarantee precise ballistics with high oxygen content for safety. The present study addresses the relevant question potential supplemental administered during on normal tissue...
Proton minibeam radiation therapy (pMBRT) is an innovative approach that highly modulates the spatial dimension of dose delivery using narrow, parallel, and submillimetric proton beamlets. pMBRT has proven its remarkable healthy tissue preservation in brain skin. This study assesses potential advantages for thoracic irradiations compared with conventional terms normal toxicity. The challenge here was influence respiratory motion on typical peak valley patterns biologic effect.
Abstract Radiation retinopathy (RR) is a major side effect of ocular tumor treatment by plaque brachytherapy or proton beam therapy. RR manifests as delayed and progressive microvasculopathy, ischemia macular edema, ultimately leading to vision loss, neovascular glaucoma, and, in extreme cases, secondary enucleation. Intravitreal anti-VEGF agents, steroids laser photocoagulation have limited effects on RR. The role retinal inflammation its contribution the microvascular damage occurring...
Calpain 3 is a calcium-dependent cysteine protease that primarily expressed in skeletal muscle and implicated limb girdle muscular dystrophy type 2A. To date, its best characterized function located within the sarcomere, but this found other cellular compartments, which suggests it exerts multiple roles. Here, we present evidence calpain involved myogenic differentiation process. In course of vitro culture myoblasts to fully differentiated myotubes, population quiescent undifferentiated...
Proton minibeam radiation therapy (pMBRT) is a new approach in proton radiotherapy, by which significant increase the therapeutic index has already been demonstrated RG2 glioma-bearing rats. In current study we investigated response of other types glioma (F98) and performed comparative evaluation tumor control effectiveness pMBRT (with different levels dose heterogeneity) versus conventional therapy. The results our showed an equivalent lifespan for all evaluated groups (conventional...
Minibeam radiation therapy (MBRT) is a type of spatial fractionated radiotherapy that uses submillimetric beams. This work reports on pilot study normal tissue response and the increase lifespan glioma-bearing rats when irradiated with tabletop x-ray system. Our results show significant widening therapeutic window for brain tumours treated MBRT: an important proportion long-term survivals (60%) coupled reduction toxicity compared conventional (broad beam) irradiations. In addition, clinical...
Re-irradiation remains a medical challenge due to the high toxicity risk. Proton minibeam radiation therapy (pMBRT), which has already demonstrated remarkable neurotoxicity reduction, could minimize that risk in central nervous system re-irradiations. The goal of this study was perform first evaluation anatomical and functional effects pMBRT rat brain Rats' brains were subjected either single conventional X-rays irradiation or additionally second with RT pMBRT. Behavioral tests carried out...