A5023641027- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- DNA Repair Mechanisms
- Radiation Detection and Scintillator Technologies
- DNA and Nucleic Acid Chemistry
- Ion-surface interactions and analysis
- Nuclear Physics and Applications
- Radiation Effects and Dosimetry
- Molecular Biology Techniques and Applications
- Effects of Radiation Exposure
- Radiopharmaceutical Chemistry and Applications
- Carcinogens and Genotoxicity Assessment
- Free Radicals and Antioxidants
- Laser-Plasma Interactions and Diagnostics
- Boron Compounds in Chemistry
- Integrated Circuits and Semiconductor Failure Analysis
- Radioactivity and Radon Measurements
- RNA Interference and Gene Delivery
- Mass Spectrometry Techniques and Applications
- Atomic and Molecular Physics
- Genomics and Chromatin Dynamics
- X-ray Spectroscopy and Fluorescence Analysis
- Glioma Diagnosis and Treatment
- Advanced X-ray and CT Imaging
- Redox biology and oxidative stress
Czech Academy of Sciences, Nuclear Physics Institute
2016-2025
Czech Academy of Sciences
2007-2025
National Radiation Protection Institute
2025
Nuclear Research Institute Rez (Czechia)
2017-2020
Czech Technical University in Prague
2007-2019
Institute of Nuclear Physics
2017-2018
Czech Academy of Sciences, Institute of Physics
2011-2018
MedAustron
2018
Medulloblastoma, the most prevalent brain tumor among children, requires a comprehensive understanding of its cellular characteristics for effective research and treatment. In this study, we focused on DAOY, permanent cell line medulloblastoma, investigated unique properties DAOY cells when cultured as floating multicellular aggregates called spheres, opposed to adherent monolayers. Through our analysis, identified distinct associated with spheres. Our findings demonstrate that spheres...
Abstract Radiation protection is a cornerstone of public health, occupational safety, patient welfare, and environmental stewardship. A strong emphasis on radiation necessary to contribute the well-being citizens, innovation sustainable growth across Europe, meet challenges by application new technologies, emerging threats. The use ionising radioactive substances has long been integral advancements in medicine, as well nuclear non-nuclear industries, with well-established applications...
Different particles with similar LET and energy may generate different types of DNA damage consequences for double-strand break repair.
To investigate the clinical implications of a variable relative biological effectiveness (RBE) on proton dose fractionation. Using acute exposures, current adoption generic, constant cell killing RBE has been shown to underestimate effect sharp increase in linear energy transfer (LET) distal regions spread-out Bragg peak (SOBP). However, experimental data for impact fractionation such scenarios are still limited.Human fibroblasts (AG01522) at 4 key depth positions SOBP maximum 219.65 MeV...
Our understanding of radiation induced cellular damage has greatly improved over the past decades. Despite this progress, there are still many obstacles to fully how interacts with biologically relevant components form observable endpoints. One hurdle is difficulty faced by members different research groups in directly comparing results. Multiple Monte Carlo codes have been developed simulate induction at DNA scale, while same time various models that describe repair processes varying levels...
Abstract Saliva, as a non-invasive and easily accessible biofluid, has been shown to contain RNA biomarkers for prediction diagnosis of several diseases. However, systematic analysis done by our group identified two problematic issues not coherently described before: (1) most the isolated originates from oral microbiome (2) amount human is comparatively low. The degree bacterial contamination showed ratios up 1:900,000, so that only about one out 900,000 copies was origin, but quality...
Craniospinal irradiation (CSI) has greatly increased survival rates for patients with a diagnosis of medulloblastoma and other primitive neuroectodermal tumors. However, as it includes exposure large volume healthy tissue to unwanted doses, there is strong concern about the complications treatment, especially children. To estimate risk second cancers effects, out-of-field dose assessment necessary. The purpose this study evaluate compare doses in pediatric CSI treatment using conventional...
The increasing risk of acute large-scale radiological/nuclear exposures population underlines the necessity developing new, rapid and high throughput biodosimetric tools for estimation received dose initial triage. We aimed to compare induction persistence different radiation exposure biomarkers in human peripheral blood vivo. Blood samples patients with indicated radiotherapy (RT) undergoing partial body irradiation (PBI) were obtained soon before first treatment then after 24 h, 48 5...
Systematic 3D mapping of out-of-field doses induced by a therapeutic proton pencil scanning beam in 300 × 600 mm3 water phantom was performed using set thermoluminescence detectors (TLDs): MTS-7 (7LiF:Mg,Ti), MTS-6 (6LiF:Mg,Ti), MTS-N (natLiF:Mg,Ti) and TLD-700 radiophotoluminescent (RPL) GD-352M GD-302M, polyallyldiglycol carbonate (PADC)-based (C12H18O7) track-etched detectors. Neutron gamma-ray doses, as well linear energy transfer distributions, were experimentally determined at 200...
Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated variants, all up-regulated after IR...
Boron has been suggested to enhance the biological effectiveness of proton beams in Bragg peak region via p +
The ELIMAIA-ELIMED beamline, powered by the L3 HAPLS petawatt laser, enables irradiation of biological samples with intermediate-energy laser-driven protons (LDP) in a multi-shot regime. In pilot radiobiological experiment, mean energy ∼24 MeV and doses up to ∼14 mGy per shot, ∼4 ns bunch duration, were used irradiate AG01522 normal human skin fibroblasts. shortest time achieved was down ∼17 min/Gy, while peak dose rates reached ∼1 × 10 −3 3.5 6 Gy/s, respectively. cells exposed ranging from...
The PIANOFORTE partnership (2022–2029) aims to enhance radiation protection for the public, patients, workers, and environment across various exposure scenarios. This European initiative addresses key barriers in health environmental risk research related ionising promotes findings that support effective policies. By building a comprehensive pan-European scientific technological foundation, ensures system remains fit-for-purpose, delivers science-based policy recommendations improved...
The lowest possible energy of proton scanning beam in cyclotron therapy facilities is typically between 60 and 100 MeV. Treatment superficial lesions requires a pre-absorber to deliver doses shallower volumes. In most the cases range shifter (RS) used, but as an alternative solution, patient-specific 3D printed compensator (BC) can be applied. A BC enables further reduction air gap consequently scattering. Such pre-absorbers are additional sources secondary radiation. aim this work was...
We report experimental results on damage induced by ionizing radiation to DNA origami triangles which are commonly used prototypes for scaffolded nanostructures. demonstrate extreme stability of upon irradiation, is caused (i) the multi-row design holding shape even after severe scaffold and (ii) reduction due protective effect folded structure. With respect radiation, structure superior that a naturally paired double helix. Present allow estimating nanostructures in applications such as...