A5041686445- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Adenosine and Purinergic Signaling
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Vaccine Coverage and Hesitancy
- CAR-T cell therapy research
- Psoriasis: Treatment and Pathogenesis
- Viral Infections and Vectors
- Immune cells in cancer
- Heat shock proteins research
- Diabetes and associated disorders
- Fire effects on ecosystems
- Influenza Virus Research Studies
- Sexual Differentiation and Disorders
- COVID-19 epidemiological studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Systemic Lupus Erythematosus Research
- Retinoids in leukemia and cellular processes
- Microbial Metabolic Engineering and Bioproduction
- Protein purification and stability
- Hematopoietic Stem Cell Transplantation
- Viral Infections and Outbreaks Research
Fundación Ciencia and Vida
2016-2025
University of Chile
2014-2025
San Sebastián University
2020-2025
Universidad Andrés Bello
2011-2020
Fundación Chile
2017
Biolog (United States)
2014
Millennium Institute for Integrative Biology
2002-2012
Millennium Institute
2001-2009
Institut Curie
2008
Inserm
2008
We demonstrate that all-trans retinoic acid (RA) induces FoxP3+ adaptive T regulatory cells (A-Tregs) to acquire a gut-homing phenotype (α4β7+ CC chemokine receptor 9+) and the capacity home lamina propria of small intestine. Under conditions favor differentiation A-Tregs (transforming growth factor–β1 interleukin 2) in vitro, inclusion RA nearly all activated CD4+ express FoxP3 greatly increases accumulation these cells. In absence RA, A-Treg is abruptly impaired by proficient antigen...
Dendritic cells (DCs) sample peripheral tissues of the body in search antigens to present T cells. This requires two processes, antigen processing and cell motility, originally thought occur independently. We found that major histocompatibility complex II-associated invariant chain (Ii or CD74), a known regulator processing, negatively regulates DC motility vivo. By using microfabricated channels mimic confined environment tissues, we wild-type DCs alternate between high low whereas...
A new method for isolating transverse tubule membranes from rabbit skeletal muscle has been developed. This procedure the advantage of being mild, fast, and producing with good yields a purified membrane fraction. The are by discontinuous sucrose density centrifugation after loading contaminating light sarcoplasmic reticulum vesicles calcium phosphate in presence ATP. Immunofluorescence staining cryostat sections psoas goat antibodies directed against shows that reacting antigens distributed...
Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation active proteases into phagosome macrophage cell line J774 indicates maturation involves progressive fusion with early and late endocytic compartments. In contrast, biogenesis bone marrow–derived dendritic cells (DCs) macrophages...
Human erythroblastic precursor cells adhere to fibronectin (Fn) but the exact nature of receptors mediating this interaction has not been characterized. In study, we report data showing that immature human erythroblasts express integrins VLA-4 and VLA-5 both these molecules act as on cells. We have recently demonstrated adhesion Fn purified CFU-E their immediate progeny preproerythroblasts was inhibited by antibodies directed against receptor (VLA-5). Here extended those results...
As it has been established that demethylation of lysine 27 histone H3 by the lysine-specific demethylase JMJD3 increases immune responses and thus elicits inflammation, we hypothesize inhibition may attenuate autoimmune disorders. We found in vivo administration GSK-J4, a selective inhibitor UTX, ameliorates severity experimental encephalomyelitis (EAE). In vitro experiments revealed anti-inflammatory effect GSK-J4 was exerted through an on dendritic cells (DCs), promoting tolerogenic...
CD39 and CD73 are ectoenzymes that dephosphorylate ATP into its metabolites; ADP, AMP, adenosine, thus considered instrumental in the development of immunosuppressive microenvironments. We have previously shown within CD8+ T cell population, naïve memory cells express ectonucleotidase, while terminally differentiated effector devoid this enzyme. This evidence suggests adenosine might exert an autocrine effect on during differentiation. To study possible role process, we compared expression...
Hantavirus cell entry is promoted by its envelope glycoproteins, Gn and Gc, through attachment fusion between viral endosomal membranes at low pH. However, the role of Gc in receptor binding has not yet been defined. In this work, a sequence presenting characteristics similar to those class II peptides (FPs) alphavirus E1 flavivirus E proteins identified within hantavirus glycoprotein. A three-dimensional comparative molecular model based on crystallographic data tick-borne encephalitis...
Autoantibodies are of central importance in the pathogenesis Ab-mediated autoimmune disorders. The murine lupus susceptibility locus Nba2 on chromosome 1 and syntenic human associated with a loss immune tolerance that leads to antinuclear Ab production. To identify gene intervals within control development autoantibody-producing B cells determine cellular components through which genes accomplish this, we generated congenic mice expressing various where for FcgammaR, SLAM, IFN-inducible...
Abstract It has recently been shown that IL-4 can educate dendritic cells (DC) to differentially affect T cell effector activity. In this study, we show also act upon DC instruct naive express the gut-associated homing receptor CCR9. Thus, generated after coculture with mesenteric lymph node (MLN)-DC a higher expression of CCR9 when activated in presence IL-4. contrast, had no effect on were polyclonally absence MLN-DC, suggesting passed through DC. Indeed, by MLN-DC from IL-4Rα−/− mice...
Maintaining the identity of Foxp3 + regulatory T cells (Tregs) is critical for controlling immune responses in gut, where an imbalance between Tregs and effector has been linked to inflammatory bowel disease. Accumulating evidence suggests that can convert into Th17 acquire phenotype. In this study, we used adoptive transfer model Ag-specific study contribution different factors reprogramming vitro -generated Treg (iTreg) IL-17-producing a mouse gut inflammation vivo . Our results show...
The acquired immune response begins with Ag presentation by dendritic cells (DCs) to naive T in a heterocellular cell-cell contact-dependent process. Although both DCs and are known express connexin43, gap junction protein subunit, the role of connexin43 on initiation cell responses remains be elucidated. In present work, we report formation junctions between their activation during DCs. cocultures cells, Lucifer yellow microinjected into is transferred adjacent transgenic CD4(+) only if...
Plasmacytoid pre-dendritic cells (pDCs) are specialized in responding to nucleic acids, and link innate with adaptive immunity. Although the response of pDCs viruses is well established, whether can respond extracellular bacteria remains controversial. Here, we demonstrate that such as Neisseria meningitidis, Haemophilus influenzae, Staphylococcus aureus activate produce IFN-α, TNF-α, IL-6, upregulate CD86 expression. We observed were present within tonsillar crypts oro-nasopharyngeal...