A5023113293- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Melanoma and MAPK Pathways
- 14-3-3 protein interactions
- Respiratory viral infections research
- Cancer Treatment and Pharmacology
- Adenosine and Purinergic Signaling
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
- Systemic Lupus Erythematosus Research
- Congenital Diaphragmatic Hernia Studies
- CAR-T cell therapy research
- Cellular Mechanics and Interactions
- Retinoids in leukemia and cellular processes
- Cellular transport and secretion
- Vaccine Coverage and Hesitancy
- Long-Term Effects of COVID-19
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Signaling Pathways in Disease
University of Chile
2016-2025
Fundación Ciencia and Vida
2001-2025
University of Genoa
2004-2021
Hôpital Sainte-Marguerite
2020
Istituto Giannina Gaslini
2017-2018
Universidad Andrés Bello
2008-2013
Millennium Institute for Integrative Biology
2002-2008
IFOM
2004
Millennium Institute
2001-2004
Centro de Estudios Científicos
2002
P66Shc regulates life span in mammals and is a critical component of the apoptotic response to oxidative stress. It functions as downstream target tumor suppressor p53 indispensable for ability stress-activated induce apoptosis. The molecular mechanisms underlying apoptogenic effect p66Shc are unknown. Here we report following three findings. (i) apoptosome can be properly activated vitro absence only if purified cytochrome c supplied. (ii) Cytochrome release after signals impaired p66Shc....
Cellular glutathione levels may exceed vitamin C by 10-fold, generating the question about real antioxidant role that low intracellular concentrations of can play in presence a vast molar excess glutathione. We characterized metabolism and its relationship with primary cultures human endothelial cells oxidatively challenged treatment hydrogen peroxide or activated undergoing respiratory burst, analyzed manner which interacts to increase capacity cells. Our data indicate that: (i) express...
Cellular immunity has classically been described as the defense mechanism for viral infections. The development of cellular or humoral immune responses will depend on a repertoire cytokines produced by numerous cells, including CD4+ and CD8+ T cells. These lymphocytes can be subdivided into 2 subsets, helper 1 (Th1) Th2, basis cytokine profiles they synthesize. Type cells produce interferon gamma (IFN-gamma), an essential in cell-mediated response. Th2 selectively interleukin 4 (IL-4) IL-5...
Although it is well established that microbial infections predispose to autoimmune diseases, the underlying mechanisms remain poorly understood. After infection, tissue-resident memory T (TRM) cells persist in peripheral organs and provide immune protection against reinfection. However, whether TRM participate responses unrelated primary such as inflammation, unknown. By using high-dimensional single-cell analysis, we identified CD4+ with a TH17 signature (termed TRM17 cells) kidneys of...
The cytotoxic effect of microcin E492, a low-molecular-mass channel-forming bacteriocin (7,887 Da) produced by strain Klebsiella pneumoniae , was characterized in HeLa cells. At low (5 μg/ml) and intermediate (10 concentrations, E492 induced biochemical morphological changes typical apoptosis, such as cell shrinkage, DNA fragmentation, extracellular exposure phosphatidylserine, caspase activation, loss mitochondrial membrane potential. Treatment with zVAD-fmk, general inhibitor, completely...
As it has been established that demethylation of lysine 27 histone H3 by the lysine-specific demethylase JMJD3 increases immune responses and thus elicits inflammation, we hypothesize inhibition may attenuate autoimmune disorders. We found in vivo administration GSK-J4, a selective inhibitor UTX, ameliorates severity experimental encephalomyelitis (EAE). In vitro experiments revealed anti-inflammatory effect GSK-J4 was exerted through an on dendritic cells (DCs), promoting tolerogenic...
Neuroinflammation constitutes a pathogenic process leading to neurodegeneration in several disorders, including Alzheimer's disease, Parkinson's disease (PD) and sepsis. Despite microglial cells being the central players neuroinflammation, astrocytes play key regulatory role this process. Our previous results indicated that pharmacologic-antagonism or genetic deficiency of dopamine receptor D3 (DRD3) attenuated neuroinflammation two mouse models PD. Here, we studied how DRD3-signalling...
Insufficient oxygen supply represents a relevant issue in several fields of human physiology and medicine. It has been suggested that the implantation photosynthetic cells can provide to tissues absence vascular supply. This approach demonstrated be successful vitro vivo models; however, no data is available about their safety patients. Here, an early phase-1 clinical trial (ClinicalTrials.gov identifier: NCT03960164, https://clinicaltrials.gov/ct2/show/NCT03960164) presented evaluate...
The human thymus is susceptible to viral infections that can severely alter thymopoiesis and compromise the mechanisms of acquired tolerance self-antigens. In humans, plasma cells residing primarily in bone marrow confer long-lasting protection common viruses by secreting antigen-specific antibodies. Since also houses B cells, we examined phenotypic complexity these thymic resident their possible protective role against infections. Using tissue specimens collected from subjects ranging age 5...
CD39 and CD73 are ectoenzymes that dephosphorylate ATP into its metabolites; ADP, AMP, adenosine, thus considered instrumental in the development of immunosuppressive microenvironments. We have previously shown within CD8+ T cell population, naïve memory cells express ectonucleotidase, while terminally differentiated effector devoid this enzyme. This evidence suggests adenosine might exert an autocrine effect on during differentiation. To study possible role process, we compared expression...
Abstract The Src homology and collagen (Src) family of adaptor proteins comprises six Shc-like encoded by three loci in mammals (Shc, Rai, Sli). are tyrosine kinase substrates, which regulate diverse signaling pathways cellular functions, including Ras proliferation (p52/p46Shc), phosphatidylinositol 3-kinase survival (p54Rai), mitochondrial permeability transition apoptosis (p66Shc). Here, we report the identification, cloning, sequence characterization a new member Shc that termed RaLP....
Autoantibodies are of central importance in the pathogenesis Ab-mediated autoimmune disorders. The murine lupus susceptibility locus Nba2 on chromosome 1 and syntenic human associated with a loss immune tolerance that leads to antinuclear Ab production. To identify gene intervals within control development autoantibody-producing B cells determine cellular components through which genes accomplish this, we generated congenic mice expressing various where for FcgammaR, SLAM, IFN-inducible...
Abstract It has recently been shown that IL-4 can educate dendritic cells (DC) to differentially affect T cell effector activity. In this study, we show also act upon DC instruct naive express the gut-associated homing receptor CCR9. Thus, generated after coculture with mesenteric lymph node (MLN)-DC a higher expression of CCR9 when activated in presence IL-4. contrast, had no effect on were polyclonally absence MLN-DC, suggesting passed through DC. Indeed, by MLN-DC from IL-4Rα−/− mice...
Maintaining the identity of Foxp3 + regulatory T cells (Tregs) is critical for controlling immune responses in gut, where an imbalance between Tregs and effector has been linked to inflammatory bowel disease. Accumulating evidence suggests that can convert into Th17 acquire phenotype. In this study, we used adoptive transfer model Ag-specific study contribution different factors reprogramming vitro -generated Treg (iTreg) IL-17-producing a mouse gut inflammation vivo . Our results show...