A5068548004- Zebrafish Biomedical Research Applications
- Neonatal Respiratory Health Research
- Congenital heart defects research
- Immune Cell Function and Interaction
- Coronary Artery Anomalies
- Hematopoietic Stem Cell Transplantation
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- IL-33, ST2, and ILC Pathways
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Immune cells in cancer
- Pancreatic function and diabetes
- Developmental Biology and Gene Regulation
- Erythrocyte Function and Pathophysiology
Institut Pasteur
2019-2026
Inserm
2019-2024
Université Paris Cité
2020-2024
Immunité et Cancer
2021-2022
Sorbonne Paris Cité
2020-2022
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2017-2021
Universidade do Porto
2017-2021
Délégation Paris 7
2020
University of Coimbra
2017
In the embryo, first hematopoietic cells derive from yolk sac and are thought to be rapidly replaced by progeny of stem cells. We used three lineage-tracing mouse models show that, contrary what was previously assumed, do not contribute significantly erythrocyte production up until birth. Lineage tracing erythromyeloid progenitors, which generate tissue resident macrophages, identified highly proliferative erythroid progenitors that differentiate after intra-embryonic injection, persisting...
Summary It was proposed that two sequential sources of intraembryonic multipotent progenitors ensure blood cell production from late gestation into adulthood, with only the latter producing self-renewing hematopoietic stem cells (HSC). How these populations differ and how they impact establishment postnatal immune system, remains poorly understood. Using complementary lineage tracing models, we showed first emerging embryonic (eMPP) are responsible for hematopoiesis. They distinct HSC do not...
Abstract The fetal liver (FL) is the main hematopoietic organ during embryonic development. FL also unique anatomical site where stem cells expand before colonizing bone marrow, they ensure life‐long blood cell production and become mostly resting. identification of different types that comprise stroma in essential to understand signals required for expansion differentiation cells. We used a panel monoclonal antibodies identify stromal 5‐laser equipped spectral flow cytometry (FCM) analyzer....
The assessment of the regenerative capacity heart has been compromised by lack surface signatures to characterize cardiomyocytes (CMs). Here, combined multiparametric marker analysis with single-cell transcriptional profiling and in vivo transplantation identify main mouse fetal cardiac populations their progenitors (PRGs). We found that CMs at different stages differentiation coexist during development. identified a population immature heat stable antigen (HSA)/ cluster 24 (CD24)+ persists...
Abstract During embryogenesis, yolk-sac and intra-embryonic-derived hematopoietic progenitors, comprising the precursors of adult stem cells, converge into fetal liver. With a new staining strategy, we defined all non-hematopoietic components liver found that hepatoblasts are major producers growth factors. We identified mesothelial novel component stromal compartment, producing Kit ligand, cytokine. A high-definition imaging dataset analyzed using deep-learning based pipeline allowed...
Summary Embryonic hematopoiesis consists of distinct waves originating in rapid succession from different anatomical locations. Hematopoietic progenitors appearing earlier than definitive hematopoietic stem cells (HSCs) play key roles fetal and postnatal life. However, their precise origin, identity the extent contribution need further clarification. To this aim, we took advantage a genetic fate-mapping strategy mice that allows labeling tracking subsets hemogenic endothelium (HE)....
Embryonic hematopoietic cells develop in the fetal liver (FL), surrounded by diverse non-hematopoietic stromal cells. However, spatial organization and cytokine production patterns of stroma during FL development remain poorly understood. Here, we characterized mapped cell populations at early (E12.5–14.5) stages, revealing that while hepatoblasts were primary source growth factors, other cells—including mesenchymal, mesothelial, endothelial cells—also contributed to this signaling network....
Abstract The epicardium is a reservoir of progenitors that give rise to coronary vasculature and stroma during development mediates cardiac vascular repair. However, its role as source in the adult mammalian heart remains unclear due lack clear lineage markers single-cell culture systems elucidate epicardial progeny cell fate. We found vivo exposure mice physiological hypoxia induced cells re-enter cycle express subset developmental genes. Multiplex single transcriptional profiling revealed...
Abstract The first hematopoietic cells are produced in the yolk sac and thought to be rapidly replaced by progeny of stem cells. Here we document that do not contribute significantly erythrocyte production up until birth. Lineage tracing sac-derived erythromyeloid progenitors, also tissue resident macrophages, shows a highly proliferative erythroblasts, after intra embryonic injection, differentiate. These similar cells, c-Myb dependent developmentally restricted as they found bone marrow....
SUMMARY Multiple waves of hematopoietic progenitors with distinct lineage potentials are differentially regulated in time and space. We show that the first thymic seeding comprise a unique population bipotent cells generate lymphoid tissue inducer invariant V γ 5 + T cells. Both populations embryonic origin induce maturation medullary epithelial Indeed, temporal depletion wave thymocytes results five-fold reduction mature cells, after birth. further these stem cell, not, yolk sac origin,...
Topic: 23. Hematopoiesis, stem cells and microenvironment Background: During embryogenesis, the hematopoietic system is formed by waves of progenitors that arise from yolk sac (YS) dorsal aorta (DA). These converge to fetal liver (FL), where they display distinct dynamics proliferation differentiation. New evidence ourselves others show YS, but not cell (HSC)-derived progenitors, drives hematopoiesis during period. Aims: Our aim identify how these two progenitor types respond environmental...
Abstract The epicardium is a reservoir of progenitors that give rise to coronary vasculature and stroma during development mediates cardiac vascular repair in lower vertebrates. However, its role as source the adult mammalian heart remains unclear due lack clear lineage markers single-cell culture systems elucidate epicardial progeny cell fate. We found vivo exposure mice physiological hypoxia induced cells re-enter cycle express subset developmental genes. Multiplex single transcriptional...
Abstract The fetal liver is the main hematopoietic organ during embryonic development. also unique anatomical site where stem cells expand before colonizing bone marrow, they ensure life-long blood cell production and become mostly resting. identification of different types that comprise stroma in essential to understand signals required for expansion differentiation cells. We used a panel monoclonal antibodies identify stromal 5-laser equipped spectral flow cytometry analyzer....
Introduction: The epicardium is a source of the coronary vasculature and stroma during heart development. Although quiescent in physiological adult heart, increasing evidences indicate potential activation differentiation into its progeny after cardiac injury; however, this remains debated. Hypothesis: We hypothesize that vivo hypoxia exposure can mimic embryonic profile prime epicardial progenitor cell to more immature profile. Methods: Perinatal BL6 Pw1 nLacz hearts were used track...
Abstract The epicardium is a reservoir of progenitors that give rise to coronary vasculature and stroma during development mediates cardiac vascular repair in lower vertebrates. However, its role as source the adult mammalian heart remains unclear due lack clear lineage markers single-cell culture systems elucidate epicardial progeny cell fate. We found vivo exposure mice physiological hypoxia induced cells re-enter cycle express subset developmental genes. Multiplex transcriptional...