A5053933151- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Developmental Biology and Gene Regulation
- Cardiac Structural Anomalies and Repair
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Fibrosis and Remodeling
- Animal Genetics and Reproduction
- NF-κB Signaling Pathways
- interferon and immune responses
- DNA Repair Mechanisms
- Congenital Heart Disease Studies
- Hippo pathway signaling and YAP/TAZ
- Hedgehog Signaling Pathway Studies
- Microtubule and mitosis dynamics
- Cell death mechanisms and regulation
- Dental Radiography and Imaging
- Oral and Maxillofacial Pathology
- dental development and anomalies
- Genomics and Chromatin Dynamics
Universidade do Porto
2013-2019
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2019
Maastricht University
2018
University of Minho
2009-2014
Cooperativa de Ensino Superior Politécnico e Universitário
2008
Amsterdam UMC Location VUmc
2007
Abstract Introduction Among the plethora of cells under investigation to restore a functional myocardium, mesenchymal stromal (MSCs) have been granted considerable interest. However, whereas beneficial effects bone marrow MSCs (BM-MSCs) in context diseased heart are widely reported, data still scarce on from umbilical cord matrix (UCM-MSCs). Herein we report effect UCM-MSC transplantation infarcted murine heart, seconded by dissection molecular mechanisms at play. Methods Human...
Abstract Although in flies the atypical cadherin Fat is an upstream regulator of Hippo signalling, closest mammalian homologue, Fat4, has been shown to regulate tissue polarity rather than growth. Here we show mouse heart that Fat4 modulates signalling restrict mutant myocardium thicker, with increased cardiomyocyte size and proliferation, this mediated by upregulation transcriptional activity Yap1, effector pathway. not required for canonical activation kinases but it sequesters a partner...
Abstract Activation of the tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) receptor pathway is a promising therapeutic strategy to selectively eradicate cancer cells, including non–small cell lung (NSCLC) cells. Recombinant human (rh) TRAIL/Apo-2L, TRAIL-encoding adenovirus, and monoclonal antibodies directed against TRAIL receptors R1 R2 were used study cytotoxicity therapy in NSCLC cells showed differential sensitivity therapy, regardless agent used. Combination treatment...
The spindle assembly checkpoint monitors the status of kinetochore-microtubule (K-MT) attachments and delays anaphase onset until full metaphase alignment is achieved. Recently, role proteins was expanded with discovery that BubR1 Bub1 are implicated in regulation K-MT attachments. One unsolved question whether Bub3, known to form cell cycle constitutive complexes both Bub1, also required for proper chromosome-to-spindle Using RNA interference high-resolution microscopy, we analyzed...
Vertebrate embryo somite formation is temporally controlled by the cyclic expression of somitogenesis clock genes in presomitic mesoderm (PSM). The believed to be an intrinsic property this tissue, operating independently embryonic midline structures and signaling molecules produced therein, namely Sonic hedgehog (Shh). This work revisits notochord contribution temporal control PSM segmentation assessing rate number somites formed molecular gene oscillations upon ablation. absence causes a...
So far, opposing outcomes have been reported following neonatal apex resection in mice, questioning the validity of this injury model to investigate regenerative mechanisms. We performed a systematic evaluation, up 180 days after surgery, pathophysiological events activated upon resection. In response cardiac injury, we observed increased cardiomyocyte proliferation remote and regions, neovascularization, local fibrosis. adulthood, resected hearts remain consistently shorter display...
The assessment of the regenerative capacity heart has been compromised by lack surface signatures to characterize cardiomyocytes (CMs). Here, combined multiparametric marker analysis with single-cell transcriptional profiling and in vivo transplantation identify main mouse fetal cardiac populations their progenitors (PRGs). We found that CMs at different stages differentiation coexist during development. identified a population immature heat stable antigen (HSA)/ cluster 24 (CD24)+ persists...
Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cell-based therapies. Although two distinct populations of CPCs reached clinical setting, more detailed studies required to portray optimal cell type therapeutic setting drive robust engraftment cardiomyogenesis after injury. Owing scarcity need reproducibility, generation faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized...
Abstract The assessment of the regenerative capacity heart has been compromised by lack surface signatures to characterize cardiomyocytes. Here, combined multiparametric marker analysis with single cell transcriptional profiling and in vivo transplantation, identify main fetal cardiac populations their progenitors. We found that cardiomyocytes at different stages differentiation co-exist during development. identified a population immature HSA/CD24 + persists throughout life that, unlike...
Objetivo: c om o intuito de auxiliar a determinacao do plano tratamento mais conservador, presente estudo avaliou in vitro eficacia dos metodos clinico (visual), radiografico e histologico no diagnostico das lesoes incipientes. Materiais metodos: foram avaliados 20 dentes molares permanentes humanos que apresentavam superficie oclusal aparentemente integra ou escurecida, porem sem cavitacao. Estes submetidos ao exame radiografico, atraves radiografias periapicais visual, por tres...