A5082778843- Congenital heart defects research
- Congenital Heart Disease Studies
- Developmental Biology and Gene Regulation
- Cardiomyopathy and Myosin Studies
- Genetic and Kidney Cyst Diseases
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Hippo pathway signaling and YAP/TAZ
- Renal and related cancers
- Cardiac Fibrosis and Remodeling
- Cardiovascular Function and Risk Factors
- RNA modifications and cancer
- Congenital Anomalies and Fetal Surgery
- Medical Image Segmentation Techniques
- Coronary Artery Anomalies
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Protist diversity and phylogeny
- Reproductive Biology and Fertility
- Cerebrovascular and genetic disorders
- Phonocardiography and Auscultation Techniques
- Genomic variations and chromosomal abnormalities
Université Paris Cité
2017-2025
Inserm
2017-2025
Institut Pasteur
2015-2025
Institut des Maladies Génétiques Imagine
2017-2024
Morphogenèse du coeur
2017-2023
Délégation Paris 5
2017-2020
Délégation Paris 6
2019
Sorbonne Paris Cité
2019
Descartes (Belgium)
2018
Centre National de la Recherche Scientifique
2006-2017
The mammalian heart develops from a primary tube, which is formed by fusion of bilateral cardiac territories in myocardial and endothelial cells have already begun to differentiate splanchnic mesoderm. A population precursors has been identified pharyngeal mesoderm, anterior the early tube. Cell labeling studies indicated that this novel territory, called field (AHF), gives rise wall outflow tract. We now report not only myocardium tract but also embryonic right ventricle are derived source....
Head muscle progenitors in pharyngeal mesoderm are present close proximity to cells of the second heart field and show overlapping patterns gene expression. However, it is not clear whether a single progenitor cell gives rise both head muscles. We now that this case, using retrospective clonal analysis which an nlaacZ sequence, converted functional nlacZ after rare intragenic recombination event, targeted αc-actin gene, expressed all developing skeletal cardiac muscle. distinguish two...
How left-right patterning drives asymmetric morphogenesis is unclear. Here, we have quantified shape changes during mouse heart looping, from 3D reconstructions by HREM. In combination with cell labelling and computer simulations, propose a novel model of looping. Buckling, when the cardiac tube grows between fixed poles, modulated progressive breakdown dorsal mesocardium. We identified sequential asymmetries at which bias buckling in opposite directions, thus leading to helical shape. Our...
Abstract Although in flies the atypical cadherin Fat is an upstream regulator of Hippo signalling, closest mammalian homologue, Fat4, has been shown to regulate tissue polarity rather than growth. Here we show mouse heart that Fat4 modulates signalling restrict mutant myocardium thicker, with increased cardiomyocyte size and proliferation, this mediated by upregulation transcriptional activity Yap1, effector pathway. not required for canonical activation kinases but it sequesters a partner...
Key molecules which regulate the formation of heart have been identified; however, mechanism cardiac morphogenesis remains poorly understood at cellular level. We adopted a genetic approach, permits retrospective clonal analysis myocardial cells in mouse embryo,based on targeting an nlaacZ reporter to α-cardiac actin gene. A rare intragenic recombination event leads clone ofβ-galactosidase-positive cells. Analysis clones different developmental stages demonstrates that and their precursors...
We show that cells of the dorsal aorta, an early blood vessel, and myotome, first skeletal muscle to form within somite, derive from a common progenitor in mouse embryo. This conclusion is based on retrospective clonal analysis, using nlaacZ reporter targeted alpha-cardiac actin gene. A rare intragenic recombination event results functional nlacZ sequence, giving rise clones beta-galactosidase-positive cells. Periendothelial vascular smooth aorta are main cell types labelled, demonstrating...
The ventricular conduction system controls the propagation of electric activity through heart to coordinate cardiac contraction. This is composed specialized cardiomyocytes organized in defined structures including central components and a peripheral Purkinje fiber network. How mammalian established during development remains controversial.To define lineage relationship between cells murine surrounding working myocytes.A retrospective clonal analysis using alpha-cardiac actin(nlaacZ/+) mouse...
The second heart field (SHF) contains progenitors of all chambers, excluding the left ventricle. SHF is patterned, and anterior region known to be destined form outflow tract right ventricle.The aim this study was map fate posterior (pSHF).We examined contribution pSHF cells, labeled by lipophilic dye at 4- 6-somite stage, regions 20 25 somites, using mouse embryo culture. Cells more cranial in contribute atrioventricular canal (AVC) atria, whereas those caudal generate sinus venosus, but...
Genetic tracing experiments and cell lineage analyses are complementary approaches that give information about the progenitor cells of a tissue. Approaches based on gene expression have led to conflicting views origin venous pole heart. Whereas heart forms from 2 sources cells, first second fields, genetic has suggested distinct for caval vein myocardium, proposed third field.To determine history myocardium at heart.We used retrospective clonal investigate segregation mouse heart,...
Congenital heart disease is the most frequent birth defect and leading cause of death for fetus in first year life. The wide phenotypic diversity congenital defects requires expert diagnosis sophisticated repair surgery. Although these have been described since seventeenth century, it was only 2005 that a consensus international nomenclature adopted, followed by an classification 2017 to help provide better management patients. Advances genetic engineering, imaging, omics analyses uncovered...
During heart morphogenesis, cardiac chambers arise by differential expansion of regions the primitive tube. This process is under control specific transcription factors such as Tbx5 and dHAND. To gain insight into cellular mechanisms that underlie cardiogenesis, we have used a retrospective clonal approach based on spontaneous recombination an nlaacZ reporter gene targeted to murine α-cardiac actin locus. We show growth myocardial cells oriented. At embryonic day (E) 10.5, shape clones...
The TGFβ secreted factor NODAL is a major left determinant required for the asymmetric morphogenesis of visceral organs, including heart. Yet, when this signaling absent, shape asymmetry, example embryonic heart loop, not fully abrogated, indicating that there are other factors regulating left–right patterning. Here, we used tailored transcriptomic approach to screen genes asymmetrically expressed in field progenitors. We thus identify Notch3 as novel left-enriched gene and validate, by...
The ventricular conduction system (VCS) coordinates the heartbeat and is composed of central components (the atrioventricular node, bundle, right left bundle branches) a peripheral Purkinje fiber network. Conductive myocytes develop from common progenitor cells with working in bimodal process lineage restriction followed by limited outgrowth. relationship between giving rise to different VCS unclear.Cell contributions were analysed combination retrospective clonal analysis, regionalized...