A5069266591- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- ATP Synthase and ATPases Research
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Autophagy in Disease and Therapy
- Neuroscience and Neural Engineering
- Cardiac Ischemia and Reperfusion
- Pancreatic function and diabetes
- Muscle metabolism and nutrition
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Neuroscience and Neuropharmacology Research
- Physiological and biochemical adaptations
- Computer Graphics and Visualization Techniques
- Neurobiology and Insect Physiology Research
- Cancer, Hypoxia, and Metabolism
- Advanced Sensor and Energy Harvesting Materials
- Nanoparticles: synthesis and applications
- Magnetic and Electromagnetic Effects
Slovak Academy of Sciences
2014-2024
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2018-2024
Centre of Biosciences of the Slovak Academy of Sciences
2006-2020
Comenius University Bratislava
2009
Laboratory Signaling and Cardiovascular Pathophysiology
2006
Centre for Human Genetics
2001
University of Tartu
2001
Inserm
2001
Mitochondrial dynamics is a recent topic of research in the field cardiac physiology. The study mechanisms involved morphological changes and mobility mitochondria legitimate since adult cardiomyocytes possess numerous which occupy at least 30% cell volume. However, architectural constraints exist cardiomyocyte that limit mitochondrial movements communication between adjacent mitochondria. Still, proteins fusion fission are highly expressed these cells could be different processes important...
Cells with high and fluctuating energy demands such as cardiomyocytes need efficient systems to link production utilization. This is achieved in part by compartmentalized transfer enzymes creatine kinase (CK). However, hearts from CK-deficient mice develop normal cardiac function under conditions of moderate workload. We have therefore investigated whether a direct functional interplay exists between mitochondria sarcoplasmic reticulum or myofilaments cells that catalyzes signal channeling...
The optic atrophy 1 (OPA1) protein is an essential involved in the fusion of mitochondrial inner membrane. Despite its high level expression, role OPA1 heart largely unknown. We investigated this Opa1+/− mice, having a 50% reduction expression cardiac tissue. In mutant function assessed by echocardiography was not significantly different from that Opa1+/+. Electron and fluorescence microscopy revealed altered morphology mice network; unexpectedly, mitochondria were larger with presence...
Abstract Although in flies the atypical cadherin Fat is an upstream regulator of Hippo signalling, closest mammalian homologue, Fat4, has been shown to regulate tissue polarity rather than growth. Here we show mouse heart that Fat4 modulates signalling restrict mutant myocardium thicker, with increased cardiomyocyte size and proliferation, this mediated by upregulation transcriptional activity Yap1, effector pathway. not required for canonical activation kinases but it sequesters a partner...
Cardiomyocyte contractile function requires tight control of the ATP/ADP ratio in vicinity myosin-ATPase and sarcoplasmic reticulum ATPase (SERCA). In these cells, main systems that provide energy are creatine kinase (CK), which catalyses phosphotransfer from phosphocreatine to ADP, direct adenine nucleotide channelling (DANC) mitochondria ATPases. However, it is not known how when complex energetic established during postnatal development. We therefore studied maturation efficacy with DANC...
Many recent studies have demonstrated the involvement of endoplasmic reticulum (ER) stress in development cardiac diseases and suggested that modulation ER response could be cardioprotective. Previously, we deacetylase Sirtuin 1 (SIRT1) attenuates promotes cardiomyocyte survival. Here, investigated whether how autophagy plays a role SIRT1-afforded cardioprotection against stress. The results revealed protective was initiated before cell death to tunicamycin (TN)-induced cells. SIRT1...
Introduction: Gold nanoparticles are promising candidates as vehicles for drug delivery systems and could be developed into effective anticancer treatments. However, concerns about their safety need to identified, addressed, satisfactorily answered. Although gold considered biocompatible nontoxic, most of the toxicology evidence originates from in vitro studies, which may not reflect responses complex living organisms. Methods: We used an animal model study long-term effects 20 nm spherical...
The role of OPA1, a GTPase dynamin protein mainly involved in the fusion inner mitochondrial membranes, has been studied many cell types, but only few studies have conducted on adult differentiated tissues such as cardiac or skeletal muscle cells. Yet OPA1 is highly expressed these cells, and could play different roles, especially response to an environmental stress like exercise. Endurance exercise increases energy demand repeated activity induces biogenesis activation fusion-fission cycles...
Endoplasmic reticulum (ER) stress has recently emerged as an important mechanism involved in the pathogenesis of cardiovascular diseases. However, molecular mechanisms by which ER leads to cardiac dysfunction remain poorly understood. In this study, we evaluated early effects induced tunicamycin (TN) mice. Echocardiographic analysis indicated that TN-induced led a significant impairment function. Electron microscopic observations revealed ultrastructural changes cardiomyocytes response...
AMP-activated protein kinase (AMPK) plays an important role in controlling energy homeostasis and is envisioned as a promising target to treat metabolic disorders. In the heart, AMPK involved short-term regulation transcriptional control of proteins metabolism. Here, we investigated whether deletion AMPKalpha2, main cardiac catalytic isoform, alters mitochondrial function biogenesis. Body weight, heart AMPKalpha1 expression were similar littermate AMPKalpha2(-/-) mice. Despite normal oxygen...
Sarcoplasmic reticulum (SR) calcium pump function requires a high local ATP/ADP ratio, which can be maintained by direct nucleotide channelling from mitochondria, and SR‐bound creatine kinase (CK)‐catalysed phosphate‐transfer phosphocreatine. We hypothesized that SR uptake supported mitochondrial channelling, but not bound CK, depends on the juxtaposition of these organelles. To test this, we studied well‐described model cytoarchitectural disorganization, muscle LIM protein (MLP)‐null mouse...
Local control of ATP/ADP ratio is essential for efficient functioning cellular ATPases. Since creatine kinase (CK) activity and mitochondrial content are reduced in heart failure (HF), cardiomyocyte ultrastructure altered, we hypothesized that these changes may affect the local energetic two major cardiac ATPases, sarcoplasmic reticulum (SR) Ca 2+ ‐ATPase (SERCA) myosin ATPase. Heart was induced by aortic stenosis rats. Electron microscopy confirmed failing cardiomyocytes had intracellular...
Progressive expansion of nanomaterials in our everyday life raises concerns about their safety for human health. Although kidneys are the primary organs xenobiotic elimination, little attention has been paid to terms nanotoxicological studies up now. Here we investigate cytotoxic and genotoxic potential four solid-core uncoated inorganic nanoparticles (TiO2NPs, SiO2NPs, Fe3O4NPs AuNPs) using renal proximal tubule epithelial TH1 cells. To mimic vivo conditions more realistic, cells were...
Fast-twitch skeletal muscle of mice deficient in cytosolic and mitochondrial creatine kinase isoforms (CK-/-) lack burst activity but can sustain prolonged contractile activity, suggesting that adaptive mechanisms regulate local adenine nucleotide turnover. We investigated whether direct energy signal channeling between mitochondria sarcoplasmic reticulum (SR) or myofilaments may exist compensate for the CK isoenzymes. Oxidative capacity fast-twitch was increased twofold CK-/- mice. Energy...
Abstract Cardiac excitation-contraction coupling relies on dyads, the intracellular calcium synapses of cardiac myocytes, where plasma membrane contacts sarcoplasmic reticulum and electrical excitation triggers release. The morphology dyads dynamics local release vary substantially. To better understand correspondence between structure functionality we estimated incidences structurally different kinetically sites tested their responsiveness to experimental myocardial injury in left...
Wolframin (Wfs1) is a membrane protein of the sarco/endoplasmic reticulum. Wfs1 mutations are responsible for Wolfram syndrome, characterized by diabetic and neurological symptoms. Although expressed in cardiac muscle, its role this tissue not clear. We have effect invalidation on calcium signalling-related processes isolated ventricular myocytes exon5-Wfs1 deficient rats (Wfs1 e5/ e5) before onset overt disease. Calcium transients contraction were measured field-stimulated using confocal...
In the present work, effect of isoproterenol on electrical properties rat heart and cytoarchitecture surviving cardiomyocytes was studied. Myocardial remodelling induced by daily administration 5 mg/kg (Iso) for 7 days. Administration resulted in a significant increase (52%) ratio left ventricular weight to body weight. ECG voltage criteria confirmed presence hypertrophy. QT interval prolongation 23% 58% found Iso rats corresponding isolated hearts, respectively. Spontaneously beating hearts...
Understanding muscle adaptation to various stimuli is difficult because of the complex nature and responses. In particular, responses perturbations in energy metabolism require careful examination, they may involve both structural functional elements. To estimate component myocyte energetic deficiency, we used transgenic mice with blocked expression mitochondrial cytosolic creatine kinases (CK). The ultrastructure was analyzed using stereological method vertical sections applied electron...
Phototoxicity is a side-effect of in vitro and vivo oxygen partial pressure (pO2) detection by luminescence lifetime measurement methods. Dichlorotris(1,10-phenanthroline)-ruthenium(ii) hydrate ([Ru(Phen)3]2+) water soluble pO2 probe associated with low phototoxicity, which we investigated the chick's chorioallantoic membrane (CAM) after intravenous or topical administration normal human coronary artery endothelial cells (HCAEC). In vivo, level intravenously injected [Ru(Phen)3]2+ decreases...