A5085113005- Ion channel regulation and function
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Cell death mechanisms and regulation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cancer therapeutics and mechanisms
- Photoreceptor and optogenetics research
- Analytical Chemistry and Sensors
- Cardiomyopathy and Myosin Studies
- Adipokines, Inflammation, and Metabolic Diseases
- IL-33, ST2, and ILC Pathways
- Photosynthetic Processes and Mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Fibroblast Growth Factor Research
- Cardiovascular Disease and Adiposity
- Nicotinic Acetylcholine Receptors Study
- Biotin and Related Studies
- Cystic Fibrosis Research Advances
- Cellular transport and secretion
- Molecular Sensors and Ion Detection
- ATP Synthase and ATPases Research
- Muscle activation and electromyography studies
University of Pavol Jozef Šafárik
2010-2022
Czech Academy of Sciences, Institute of Physics
2013
Utah State University
2007-2011
Colorado State University
2001
University of Cincinnati Medical Center
1997
University of Cincinnati
1996
Slovak Academy of Sciences
1996
A ClC-2G(2 alpha) Cl- channel was identified to be present in human lung and stomach, a partial cDNA for this cloned from fetal library. full-length expressible constructed by ligation of mutagenized rabbit with the cDNA, expressed oocytes, characterized at single-channel level. Adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA) treatment increased probability opening (Po). After PKA activation, exhibited linear (r = 0.99) current-voltage curve slope conductance 22.1 +/- 0.8...
Potential links between metabolic derangements and adipose tissue (AT) inflammation in patients with chronic obstructive pulmonary disease (COPD) are unexplored. We investigated AT expressions of interleukin (IL)-6, tumor necrosis factor (TNF)-α, CD68 (macrophage cell surface receptor), caspase-3, Bax, their relationships to the phenotype nine cachectic, 12 normal-weight, overweight, 11 obese COPD (age<mml:math...
Rabbit and human ClC-2G Cl − channels are voltage sensitive activated by protein kinase A low extracellular pH. The objective of the present study was to investigate mechanism involved in acid activation channel determine which amino residues play a role this activation. Channel open probability ( P o ) at ±80 mV holding potentials increased fourfold concentration-dependent manner with H + concentration (that is, pH, pH trans ), an apparent acidic dissociation constant 4.95 ± 0.27....
Apoptosis is a key process in the development and maintenance of tissue homeostasis.This controlled cell death tightly regulated by balance between survival damage signals.We focused our attention towards one apoptotic pathway, intrinsic mitochondrial where Bcl-2 family proteins plays major role.We are particularly interested two pro-apoptotic players Bak Bax from this family.Here we investigated their role apoptosis triggered photodynamic action.Targeted therapy (PDT) promising approach to...
Phototoxicity is a side-effect of in vitro and vivo oxygen partial pressure (pO2) detection by luminescence lifetime measurement methods. Dichlorotris(1,10-phenanthroline)-ruthenium(ii) hydrate ([Ru(Phen)3]2+) water soluble pO2 probe associated with low phototoxicity, which we investigated the chick's chorioallantoic membrane (CAM) after intravenous or topical administration normal human coronary artery endothelial cells (HCAEC). In vivo, level intravenously injected [Ru(Phen)3]2+ decreases...
Many types of cancer such as prostate cancer, myeloid leukemia, breast glioblastoma display strong chemo resistance, which is supported by enhanced expression multiple anti-apoptotic Bcl-2, Bcl-XL and Mcl-1 proteins. The viable anti-cancer strategies are based on developing Bcl-2 proteins inhibitors, BH3 mimetics. Our focus in past years has been the investigating a new potential mimetic, Hypericin (Hyp). Hyp naturally occurring photosensitive compound used photodynamic therapy diagnosis. We...
Ca 2+ ‐dependent modulation via calmodulin, with consensus CaM‐binding IQ motif playing a key role, has been documented for most high‐voltage‐activated channels. The skeletal muscle v 1.1 also exhibits ‐/CaM‐dependent modulation. Here, whole‐cell current, transient, and maximal, immobilization‐resistant charge movement ( Q max ) recordings were obtained from cultured mouse myotubes, to test role of in function 1.1. effect introducing mutation (IQ AA) into was examined. In dysgenic myotubes...
1. Voltage changes and intramembrane charge movements in the transverse tubule membranes (T‐system) of frog fast twitch muscle fibres were compared using potentiometric dye WW‐375 a Vaseline‐gap voltage clamp. As shown previously, reports dynamic surface potential change that occurs on myoplasmic face T‐system when macroscopic applied across membrane exceeds mechanical threshold (about ‐60 mV). 2. The dependence extra movement found to be similar. Both activated with sigmoid centred around...