A5068284412- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- Cell death mechanisms and regulation
- Cancer, Hypoxia, and Metabolism
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Lexicography and Language Studies
- Autophagy in Disease and Therapy
- Syntax, Semantics, Linguistic Variation
- Trace Elements in Health
- Language, Discourse, Communication Strategies
- Electron Spin Resonance Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Synthesis and properties of polymers
- Bone Tissue Engineering Materials
- Ocular Infections and Treatments
- Swearing, Euphemism, Multilingualism
- Cancer-related Molecular Pathways
- Phagocytosis and Immune Regulation
- IoT-based Smart Home Systems
- Protein Kinase Regulation and GTPase Signaling
- Antifungal resistance and susceptibility
- Cancer Treatment and Pharmacology
- Legal Language and Interpretation
University of Pavol Jozef Šafárik
2007-2025
University of the Basque Country
2023
Institute of Physics of the Slovak Academy of Sciences
2022
Bioscience (Slovakia)
2016
Czech Academy of Sciences
2005-2006
Czech Academy of Sciences, Institute of Molecular Genetics
2005-2006
Abstract The dependence of the uptake hypericin (Hyp) by human glioma U‐87 MG cells on level expression low‐density lipoprotein (LDL) receptors has been studied in this work. A special role LDL receptor‐pathway for Hyp delivery to presence was revealed substantial increase situation, when number cell surface elevated. Moreover, colocalization experiments showed lysosomal localization following and that concentration these organelles enhanced with elevated incubation medium contained LDL....
The aim of this study is to show that SR-FTIRM can be successfully applied for the detection early apoptotic processes in human glioma cells (U-87 MG). apoptosis was induced by photodynamic action after irradiation either photosensitizer hypericin (Hyp) or a complex Hyp with low-density lipoproteins (Hyp/LDL) incorporated into cells. differences between infrared (IR) spectra non-treated and photodynamically treated U-87 MG are mainly manifested position Amide I vibrational band, sensitive...
Abstract. We tested the effect of iron deprivation on cell death induction in human Raji cells pre‐adapted to differing availability extracellular iron. Iron was achieved by incubation a defined iron‐free medium. Original have previously been adapted long‐term culture medium with 5 µg/ml iron‐saturated transferrin as source Raji/lowFe were derived from original subsequent adaptation 50 µ m ferric citrate Raji/lowFe‐re re‐adaptation transferrin‐containing (5 µg/ml) induced both and cells;...
A study of hypericin (Hyp) interaction with mitochondria isolated from U-87 MG glioma cells as well the time-resolved measurement singlet oxygen (1O2) formation and annihilation after illumination Hyp/mitochondria complex is presented in this work. Interaction between Hyp was studied by steady-state UV–vis absorption fluorescence spectroscopy. high concentration leads to aggregation compound inside relative population monomeric (biologically active) form decreases concomitantly approximately...
Low-density lipoproteins (LDL), a natural <i>in vivo</i> carrier of cholesterol in the vascular system, play key role delivery hydrophobic photosensitizers (pts) to tumor cells photodynamic therapy (PDT) cancer. To make this system even more efficient, we have constructed nano-delivery by coating LDL surface polyethylene glycol (PEG) and dextran. Fluorescence spectroscopy confocal fluorescence imaging were used characterize redistribution hypericin (Hyp), potent pts, loaded LDL/PEG...
This research investigates the suitability of printed polyamide 12 (PA12) and its dyed version to support cells in bioengineering applications. For this purpose, human gingival fibroblasts (hGF06) were cultured on PA-12 scaffolds that 3D-printed by Multi Jet Fusion (MJF). The study examined direct cultivation MJF-printed cell culture effect leachate MJF cells. article presents surface treatment PA12 material used 3D printing automatic staining vitality proliferation vitro. a unique device...
Low-density lipoproteins (LDL) and high-density (HDL) are attractive natural occurring vehicles for drug delivery targeting to cancer tissues. The capacity of both types the bind hydrophobic drugs their functionality as carriers have been examined in several studies it has also shown that mixing anticancer with LDL or HDL before administration led an increase cytotoxic effects comparison when were administered alone. However, a difficult isolation large quantity from biological organism well...
Resistance against commonly used antibiotics is a serious clinical problem in recent medical practice. There exist several bacterial strains which the possibilities of their inhibition are very limited due to multidrug resistance. Antimicrobial photodynamic therapy (aPDT) represents an option how effectively suppress growth resistant pathogens. In this work we have studied interactions potent photosensitizer hypericin (Hyp) with hospital-related gram positive (Gram+) and negative (Gram-)...
Low density lipoproteins (LDL) are considered as suitable natural in vivo delivery system for hydrophobic photosensitizers (pts) such hypericin (Hyp) and it was shown that over expression of LDL-receptors tumor cells can be used specific targeting. Activation pts by irradiation results a formation reactive oxygen species (ROS) at the place light application starts destructive mechanism. PKCα plays key role cell survival its overexpression observed glioma lines. In present study we aim to...
The submitted paper deals with analysis of grammatical means textual cohesion in English a specific genre legal register, i.e. appellate court decisions. theoretical framework is the theory introduced by Halliday and Hasan their work Cohesion (1976), which considers only intersentence cohesive ties to be textually distinguishes four categories cohesion: reference, substitution, ellipsis conjunction. In analysed texts, all instances these are identified statistically evaluated. aim twofold....
W artykule zaprezentowano wyniki syntaktycznej analizy porównawczej czterech typów konstrukcji typu semi-clause w dwóch korpusach, składających się z tekstów napisanych angielskim języku prawa odmiany brytyjskiej, tj. aktach parlamentu oraz wyrokach apelacyjnych. Autorka skupiła na różnicach zastosowaniu poszczególnych tych obu korpusach (zarówno ilościowych jak i ich funkcjach syntaktycznych) interpretacji funkcjonalnej. Wyniki ilościowe przeprowadzonej dowodzą, że średnia liczba zdaniu...
Abstract The study investigates the acquisition of telicity in L2 English by L1 Slovak speakers as a function proficiency (measured cloze test score), exposure (operationalized length stay an English-speaking country) and instruction type (monolingual vs. bilingual). Telicity judgments were collected from learners ( n = 50) control group American native 15) two offline acceptability judgment tasks. Two types encoding examined: (1) contribution [±quantized] feature object argument to...