A5036920685- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Liver Disease Diagnosis and Treatment
- Optical Imaging and Spectroscopy Techniques
- Protein Interaction Studies and Fluorescence Analysis
- Free Radicals and Antioxidants
- Aluminum toxicity and tolerance in plants and animals
- Gold and Silver Nanoparticles Synthesis and Applications
- Antibiotic Resistance in Bacteria
- Liver Disease and Transplantation
- Molecular spectroscopy and chirality
- Advanced Fluorescence Microscopy Techniques
- Porphyrin and Phthalocyanine Chemistry
- Biosensors and Analytical Detection
- Nanoparticles: synthesis and applications
- Organ Transplantation Techniques and Outcomes
- Trace Elements in Health
- Cancer, Hypoxia, and Metabolism
- Natural Compound Pharmacology Studies
- Kidney Stones and Urolithiasis Treatments
- Photochromic and Fluorescence Chemistry
- Skin Protection and Aging
- Analytical Chemistry and Sensors
Inserm
2013-2024
Université Paris-Saclay
2021-2024
Université Paris-Sud
2013-2023
Synchrotron soleil
2008-2021
Physiopathologie et Epidémiologie des Maladies Respiratoires
2016
Erasmus MC
2010-2014
Erasmus University Rotterdam
2009-2014
Laboratoire d'études sur les monothéismes
2014
Ruhr University Bochum
2013
Veneto Institute of Molecular Medicine
2013
Autofluorescence spectroscopy is a powerful tool for molecular histology and following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, mostly limited to visible near infrared excitation of samples. Several interesting naturally occurring fluorophores can be excited UV deep (DUV), but cannot monitored cellulo nor vivo due lack available instruments working this wavelength range. To fulfil need, we have developed...
3-Methoxy-17α-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that single core multimodal probe imaging – SCoMPI can be efficiently grafted onto allowing its tracking in two breast cancer cell lines, MDA-MB-231 MCF-7 fixed cells. Correlative studies based on luminescence (synchrotron UV spectromicroscopy, wide field confocal fluorescence microscopies) vibrational (AFMIR, synchrotron FTIR...
A molecular definition of the mechanism conferring bacterial multidrug resistance is clinically crucial and today methods for quantitative determination uptake antimicrobial agents with single cell resolution are missing. Using naturally occurring fluorescence antibacterial after deep ultraviolet (DUV) excitation, we developed a method to non-invasively monitor quinolones in bacteria. Our approach based on DUV microscope coupled synchrotron beamline providing tuneable excitation from 200 600...
We describe the incorporation of a single-fiber reflectance spectroscopy probe into endoscopic ultrasound fine-needle aspiration (EUS-FNA) procedure utilized for lung cancer staging. A mathematical model is developed to extract information about physiological and morphological properties lymph tissue from spectra, e.g., microvascular saturation, blood volume fraction, bilirubin concentration, average vessel diameter, Mie slope. Model analysis data clinical pilot study shows that measurement...
Abstract The dependence of the uptake hypericin (Hyp) by human glioma U‐87 MG cells on level expression low‐density lipoprotein (LDL) receptors has been studied in this work. A special role LDL receptor‐pathway for Hyp delivery to presence was revealed substantial increase situation, when number cell surface elevated. Moreover, colocalization experiments showed lysosomal localization following and that concentration these organelles enhanced with elevated incubation medium contained LDL....
Abstract Background and Objective Foslip® Fospeg® are liposomal formulations of the photosensitizer mTHPC, intended for use in Photodynamic Therapy (PDT) malignancies. Foslip consists mTHPC encapsulated conventional liposomes, Fospeg pegylated liposomes. Possible differences tumor fluorescence vasculature kinetics between Foslip, Fospeg, Foscan® were studied using rat window‐chamber model. Material Methods In 18 rats a dorsal skin fold window chamber was installed mammary carcinoma...
Non-alcoholic fatty liver disease (NAFLD) is a frequent lesion associated with obesity, diabetes and the metabolic syndrome. The hallmark feature of steatosis, which intra-cellular accumulation lipids resulting in formation vesicles hepatocytes. Steatosis precursor steatohepatitis, condition that may progress to hepatic fibrosis, cirrhosis primary cancer. We addressed potential Fourier transform-infrared (FTIR) microspectroscopy for grading steatosis on frozen tissue sections. use bright...
By means of UV-VIS absorption and fluorescence spectroscopy, we demonstrate that the photosensitizer hypericin (Hyp) interacts nonspecifically with low-density lipoproteins (LDL), most probably lipid fraction LDL. The molar ratio monomeric Hyp binding to nonoxidized LDL mildly oxidized is 30:1. Increasing concentration further leads formation aggregates inside molecule. We also photoactivated oxidizes in a light dose excitation wavelength dependent manner. level oxidation depends on amount...
Hepatocarcinogenesis is a multistep process characterized in patients with chronic liver diseases by spectrum of hepatic nodules that mark the progression from regenerative to dysplastic lesions followed hepatocellular carcinoma (HCC). The differential diagnosis between precancerous and early HCC still represents challenge for both radiologists pathologists. We addressed potential Fourier transform-infrared (FTIR) microspectroscopy grading cirrhotic on frozen tissue sections. study was...
New peptide molecules with metal binding abilities proved to be active against multidrug resistant clinical isolates. One of them labeled a dansylated lysine has been imaged inside single-multidrug bacteria cells by deep ultraviolet fluorescence, showing heterogeneous subcellular localization. The fluorescence intensity is clearly related the accumulation drug bacteria, being dependent both on its concentration and incubation time cells.
Photodynamic therapy (PDT) is an established treatment modality, used mainly for anticancer that relies on the interaction of photosensitizer, light and oxygen. For pathologies in certain anatomical sites, improved targeting photosensitizer necessary to prevent damage healthy tissue. We report a novel dual approach targeted PDT (vascular cellular targeting) utilizing expression neuropeptide somatostatin receptor (sst2) tumor neovascular-endothelial cells. synthesized two conjugates...
DUV fluorescence imaging of<italic>E. coli</italic>cells incubated with tryptophan functionalized silver nanoparticles.
Wilson's disease (WD) is a rare autosomal recessive due to mutations of the gene encoding copper-transporter ATP7B. The diagnosis hampered by variability symptoms induced copper accumulation, inconstancy pathognomonic signs and absence reliable diagnostic test. We investigated potential X-ray fluorescence (XRF) that allows quantitative analysis multiple elements. Studies were performed on animal models using Wistar rats (n = 10) Long Evans Cinnamon (LEC) 11), human samples including normal...
We present an optical method based on fluorescence spectroscopy for measuring chromophore concentrations in vivo. Fluorescence differential path length (FPDS) determines concentration the intensity corrected absorption. The of photosensitizer m-THPC (Foscan®) was studied vivo normal rat liver, which is highly vascularized and therefore absorbing. Concentration estimates measured by FDPS liver are compared with chemical extraction. Twenty-five rats were injected 0.3 mg/kg m-THPC. In...
A major challenge in biomedical optics is the accurate quantification of vivo fluorescence images. Fluorescence imaging often used to determine pharmacokinetics photosensitizers for photodynamic therapy. Often, however, this type does not take into account differences and changes tissue volume optical properties under interrogation. To address problem, a ratiometric method was developed applied monitor photosensitizer meso-tetra(hydroxyphenyl) chlorin (mTHPC) rat skin-fold observation...
Abstract Fluorescence binding measurements and molecular modeling were employed to study the interaction of hypericin (Hyp) with human (HSA), rat (RSA), bovine (BSA) serum albumins. emission data show solubility Hyp increasing in order BSA, HSA, RSA. Molecular was used construct detailed structural models complexes explain differences properties Hyp. It shown that structures Hyp/HSA Hyp/RSA are more similar some aspects different from those found for Hyp/BSA complex. The role amino acid...