A5009908907- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Lymphoma Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- S100 Proteins and Annexins
- Nanocluster Synthesis and Applications
- Pneumonia and Respiratory Infections
- Gestational Diabetes Research and Management
- Pregnancy and preeclampsia studies
- Zebrafish Biomedical Research Applications
- Medical Imaging and Pathology Studies
- Antimicrobial Peptides and Activities
- interferon and immune responses
- Cell death mechanisms and regulation
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2025
Universidade do Porto
2014-2025
Polytechnic Institute of Porto
2024-2025
Instituto de Biologia Molecular e Celular
2016-2025
Abstract Sepsis results from systemic, dysregulated inflammatory responses to infection, culminating in multiple organ failure. Here, we demonstrate the utility of CD5L for treating experimental sepsis caused by cecal ligation and puncture (CLP). We show that CD5L’s important features include its ability enhance neutrophil recruitment activation increasing circulating levels CXCL1, promote phagocytosis. CD5L-deficient mice exhibit impaired compromised bacterial control, rendering them...
γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN are generated in the mouse thymus, but molecular regulators of their peripheral functions not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter strain to analyze at unprecedented depth transcriptomes pure γδ17 cell versus γδIFN populations from lymph nodes. Within a very high fraction differentially expressed genes, identify panel 20 new signature genes steady-state cells, which...
The scavenger receptor cysteine-rich (SRCR) family comprises a group of membrane-attached or secreted proteins that contain one more modules/domains structurally similar to the membrane distal domain type I macrophage receptor. Although no all-inclusive biological function has been ascribed SRCR family, some these receptors have shown recognize pathogen-associated molecular patterns (PAMP) bacteria, fungi other microbes. SSc5D is recently described soluble produced by monocytes/macrophages...
Hypersensitivity pneumonitis (HP) is an interstitial lung disease with diverse clinical features that can present a fibrotic phenotype similar to idiopathic pulmonary fibrosis (IPF) in genetically predisposed individuals. While several single nucleotide polymorphisms (SNPs) have been associated IPF, the genetic factors contributing HP (fHP) remain poorly understood. This study investigated association of
Pulmonary fibrosis (PF) represents the pathologic end stage of several interstitial lung diseases (ILDs) associated with high morbidity and mortality rates. However, current treatments can only delay disease progression rather than provide a cure. The role inflammation in PF is well-established, but new insights into immune regulation are fundamental for developing more efficient therapies. c-MET signaling has been implicated migratory capacity effector functions cells. Nevertheless, this...
Abstract Background CD6 is one of many cell surface receptors known to regulate signal transduction upon T activation. However, whether mediates costimulatory or inhibitory signals controversial. When cells engage with antigen presenting (APCs), interacts its ligand CD166 at the cell–cell interface while cytosolic tail assembles a complex signalosome composed adaptors and effector enzymes, that may either trigger activating signaling cascades, instead modulate intensity signaling. Except for...
In recent years molecules involved on the immune synapse became successful targets for therapeutic modulation. CD6 has been extensively studied, yet, results regarding biology have controversial, in spite of ubiquitous presence this molecule virtually all CD4 T cells. We investigated outcome murine and human antibodies targeting domain 1. found that CD6-targeting had a major impact functional specialization cells, both murine. Differentiation cells towards Foxp3+ Treg fate was prevented with...
Since the pioneering discoveries, by Nobel laureates Jules Hoffmann and Bruce Beutler, that Toll Toll-like receptors can sense pathogenic microorganisms initiate, in vertebrates invertebrates, innate immune responses against microbial infections, many other families of pattern recognition (PRRs) have been described. One such receptor clusters is composed by, if not all, at least several members scavenger cysteine-rich (SRCR) superfamily. Many SRCR proteins are plasma membrane cells; however,...
Summary The characterization of the architecture, structure and extracellular interactions CD 6 glycoprotein, a transmembrane receptor expressed in medullary thymocytes all mature T‐cell populations, has been enhanced by existence monoclonal antibodies ( mA bs) that specifically recognize various scavenger cysteine‐rich SRCR ) domains ectodomain. Using engineered isoforms including or excluding each three domains, either at membranes cells soluble forms, we provide conclusive definitive...
Abstract Background T-cell membrane scaffold proteins are pivotal in T cell function, acting as versatile signaling hubs. While CD6 forms a large intracellular signalosome, it is distinguished from typical scaffolds like LAT or PAG by possessing substantial ectodomain that binds CD166, well-characterized ligand expressed on most antigen-presenting cells (APC), through the third domain (d3) of extracellular region. Although intact form abundant cells, an isoform lacking d3 (CD6∆d3)...
Embryonic hematopoietic cells develop in the fetal liver (FL), surrounded by diverse non-hematopoietic stromal cells. However, spatial organization and cytokine production patterns of stroma during FL development remain poorly understood. Here, we characterized mapped cell populations at early (E12.5–14.5) stages, revealing that while hepatoblasts were primary source growth factors, other cells—including mesenchymal, mesothelial, endothelial cells—also contributed to this signaling network....
Abstract T-cell membrane scaffold proteins play important roles in T cell biology, functioning as multi-functional signaling hubs. CD6 assembles a large intracellular signalosome but, unlike typical membrane-attached scaffolds like LAT or PAG, it has sizeable ectodomain that binds well-characterized ligand, CD166. It is unclear whether net inhibitory costimulatory functions how its influences these activities. To explore questions, we dissected the of extracellular and cytoplasmic regions...
Background: Rheumatoid Arthritis (RA) is an aggressive auto-immune disease characterized by synovial hyperplasia and chronic inflammation. The main players of RA pathogenesis are T-cell B-cell dependent pathways some myeloid cells also abundant in the tissue. However, how inflammation initiated, propagated maintained remains controversial. Unbiased proteomic reports revealed enrichment scavenger receptor CD5L, a component serum tissues arthritic patients.1 Upon secretion, this blood...
Abstract The global burden of sepsis, with an estimated 49 million cases and 11 deaths in 2017, often passes unnoticed to the general public even though it is direct cause nearly 20% all worldwide. This unawareness perhaps due misconceptions, or miscoding reporting ultimate causes death, as many diseases not actual infectious agent that biggest harm. Rather, uncontrolled inflammation leading septic shock most menacing manifestation associated infections, becomes deadly serious once has...