A5084882474- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Immunodeficiency and Autoimmune Disorders
- Cancer Immunotherapy and Biomarkers
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CAR-T cell therapy research
- S100 Proteins and Annexins
- Cytokine Signaling Pathways and Interactions
- Cancer-related gene regulation
- Immune cells in cancer
- Inflammatory Bowel Disease
- Galectins and Cancer Biology
- Receptor Mechanisms and Signaling
- Mycobacterium research and diagnosis
Universidade do Porto
2011-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2025
Instituto de Biologia Molecular e Celular
2015-2025
Unichristus
2017
Universidade de Fortaleza
2017
Hospital Universitário da Universidade de São Paulo
2015
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2014
Institute of Biomedical Science
2012
Cell and Gene Therapy Catapult
2012
University of Oxford
1993-1999
Abstract Sepsis results from systemic, dysregulated inflammatory responses to infection, culminating in multiple organ failure. Here, we demonstrate the utility of CD5L for treating experimental sepsis caused by cecal ligation and puncture (CLP). We show that CD5L’s important features include its ability enhance neutrophil recruitment activation increasing circulating levels CXCL1, promote phagocytosis. CD5L-deficient mice exhibit impaired compromised bacterial control, rendering them...
γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN are generated in the mouse thymus, but molecular regulators of their peripheral functions not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter strain to analyze at unprecedented depth transcriptomes pure γδ17 cell versus γδIFN populations from lymph nodes. Within a very high fraction differentially expressed genes, identify panel 20 new signature genes steady-state cells, which...
Calreticulin is an endoplasmic reticulum resident molecule known to be involved in the folding and assembly of major histocompatibility complex (MHC) class I molecules. In present study, expression calreticulin was analyzed human peripheral blood T lymphocytes. Pulse-chase experiments [35S]methionine-labeled cell blasts showed that associated with several proteins suggested it expressed at surface. Indeed, 60-kDa labeled by surface biotinylation precipitated from activated cells together a...
Chromosome segregation requires sister chromatid resolution. Condensins are essential for this process since they organize an axial structure where topoisomerase II can work. How separation is coordinated with chromosome condensation and decatenation activity remains unknown. We combined four-dimensional (4D) microscopy, RNA interference (RNAi), biochemical analyses to show that plays role in process. Either depletion of or exposure specific anti-topoisomerase inhibitors causes centromere...
Abstract The T lineage glycoprotein CD6 is generally considered to be a costimulator of T‐cell activation. Here, we demonstrate that significantly reduces early and late responses upon superantigen stimulation or TCR triggering by Abs. Measuring calcium mobilization in single cells responding superantigen, found human expressing rat react less well compared with not the exogenous receptor. When cytoplasmic domain was removed, were recovered, indicating inhibitory properties are attributable...
Methotrexate is the first line of treatment rheumatoid arthritis. Since many patients become unresponsive to methotrexate treatment, only very expensive biological therapies are effective and increased tolerance strategies need be identified. Here we propose encapsulation in a new liposomal formulation using hydrophobic fragment surfactant protein conjugated linker folate enhance their efficacy. In this study aim evaluate efficiency system treat arthritis, by targeting receptor β present at...
The scavenger receptor cysteine-rich (SRCR) superfamily comprises a highly conserved group of extracellular soluble and membrane-attached glycoproteins that function as pattern recognition receptors against diverse range pathogenic determinants. While considerable attention has been devoted to their roles in microbial recognition, this perspective aims emphasize the significant, yet previously overlooked, anti-inflammatory properties circulating SRCR proteins, which may be even more crucial...
Abstract Minimising suffering is an ethical and legal requirement in animal research. This particularly relevant for research on models of sepsis septic shock, which show rapid progression towards severe stages death. Specific reliable criteria signalling non-recovery points can be used as humane endpoints, beyond a study cannot allowed to progress, thus preventing avoidable suffering. Body temperature key indicator assessing health welfare has been suggested have potential monitoring the...
In T lymphocytes, CD2 forms part of a loosely associated membrane complex which includes the cell receptor (TcR) for antigen, CD3 subunits, CD4 or CD8, CD5 and protein tyrosine kinases p56lck p59fyn. The interaction with in this provides possible mechanism transmembrane signal transduction by CD2. We have investigated whether is dependent on other known members complex, an independent association can be observed. Using vitro kinase assays immune complexes precipitated from lysates, we...
Understanding the component stoichiometry of T cell antigen receptor (TCR) triggering apparatus is essential for building realistic models signal initiation. Recent studies suggesting that TCR and other signaling-associated proteins are preclustered on resting cells relied measurements behavior membrane at interfaces with functionalized glass surfaces. Using fluorescence recovery after photobleaching, we show that, compared apical surface, mobility TCRs significantly reduced Jurkat...
T lymphocytes stimulated through their antigen receptor (TCR) preferentially express mRNA isoforms with shorter 3´ untranslated regions (3´-UTRs) derived from alternative pre-mRNA cleavage and polyadenylation (APA). However, the physiological relevance of APA programs remains poorly understood. CD5 is a T-cell surface glycoprotein that negatively regulates TCR signaling onset activation. plays pivotal role in mediating outcomes cell survival or apoptosis, may prevent both autoimmunity...
Abstract The great majority of mammalian genes yield multiple transcripts arising from differential mRNA processing, but in very few instances have alternative forms been assigned distinct functional properties. We cloned and characterized a new isoform the accessory molecule CD6 that lacks CD166 binding domain is expressed rat human primary cells. novel isoform, CD6Δd3, results exon 5 skipping consequently third scavenger receptor cysteine-rich (SRCR) CD6. Differential expression SRCR 3...
Specific folate receptors are abundantly overexpressed in chronically activated macrophages and most cancer cells. Directed receptor targeting using liposomes is usually achieved linked to a phospholipid or cholesterol anchor. This link formed large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses hydrophobic fragment of surfactant protein D folate. Our proposed small 4 amino acid residue linker. The peptide conjugate inserts...
Abstract Cell surface glycoproteins anchored to the plasma membrane via glycosyl‐phosphatidylinositol (GPI) structures, and hence having no cytoplasmic domains, can nevertheless transmit activation signals in lymphocytes. By immunoprecipitation from detergent lysates vitro immune complex kinase reactions GPI‐anchored molecules Thy‐1 CD48 are shown be associated with multimolecular complexes of phosphoproteins including protein tyrosine kinases p56 lck: p60 fyn both rat mouse thymocytes....
Triggering of the T cell receptor initiates a signaling cascade resulting in activation cell. These signals are integrated alongside those from triggering other receptors whose function is to modulate overall response. CD5 an immunotyrosine-based inhibition motif-bearing that antagonizes overt response by recruiting inhibitory intracellular mediators such as SHP-1, RasGAP, or Cbl. We now propose effects also mediated parallel pathway functions at level Fyn, kinase generally associated with...
Liposomes and protein based nanoparticles were tuned with different polymers glycolipids to improve stealth thus decrease their clearance by macrophages. coated polyethylene glycol (PEG) brain-tissue-derived monosialoganglioside (GM1). Bovine serum albumin (BSA) produced incorporating a PEGylated surfactant (PEG-surfactant). All obtained monodisperse, sizes ranging from 80 120 nm, zeta-potential close zero. The presented strategies lead of internalization levels These surface modified could...
Abstract Cell surface proteins of the transmembrane 4 superfamily (TM4SF) are a newly characterized family which presumed to span plasma membrane four times. The function this molecules is poorly understood, but based on monoclonal antibody studies there some evidence that they may be involved in signal transduction and regulation cell proliferation, differentiation, or both, number different types. CD53 member expressed leukocytes, transduces activation signals through unknown mechanisms...