A5041140268- Malaria Research and Control
- Heme Oxygenase-1 and Carbon Monoxide
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Neonatal Health and Biochemistry
- Cannabis and Cannabinoid Research
- Metalloenzymes and iron-sulfur proteins
- Electrocatalysts for Energy Conversion
- Complement system in diseases
- Immunotherapy and Immune Responses
- Thermal Regulation in Medicine
- Microbial Fuel Cells and Bioremediation
- Oxidative Organic Chemistry Reactions
- IL-33, ST2, and ILC Pathways
- Influenza Virus Research Studies
- bioluminescence and chemiluminescence research
- Advanced battery technologies research
- Academic Research in Diverse Fields
- Aquaculture disease management and microbiota
- Metal complexes synthesis and properties
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- Immune Response and Inflammation
Instituto Gulbenkian de Ciência
2007-2025
Instituto de Medicina Molecular João Lobo Antunes
2023
University of Lisbon
2007-2021
Hospital de Santa Maria
2011
Universidade Nova de Lisboa
1997-2002
Rede de Química e Tecnologia
2002
Northwestern University
1997-2001
ABSTRACT Cerebral malaria is the most severe complication of human infection with Plasmodium falciparum . It was shown that berghei ANKA-induced cerebral prevented in 100% mice depleted CD8 + T cells 1 day prior to development neurological signs. However, importance parasites brains these never clearly investigated. Moreover, relevance this model has been questioned many times, especially concerning relative leukocytes versus parasitized erythrocytes sequestered brain. Here, we show...
γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN are generated in the mouse thymus, but molecular regulators of their peripheral functions not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter strain to analyze at unprecedented depth transcriptomes pure γδ17 cell versus γδIFN populations from lymph nodes. Within a very high fraction differentially expressed genes, identify panel 20 new signature genes steady-state cells, which...
The spectrum of the clinical presentation and severity malaria infections is broad, ranging from uncomplicated febrile illness to severe forms disease such as cerebral (CM), acute lung injury (ALI), respiratory distress syndrome (ARDS), pregnancy-associated (PAM) or anemia (SA). Rodent models that mimic human CM, PAM SA syndromes have been established. Here, we show DBA/2 mice infected with P. berghei ANKA constitute a new model for malaria-associated ALI. Up 60% showed dyspnea, airway...
Severe forms of malaria infection, such as cerebral (CM) and acute lung injury (ALI), are mainly caused by the apicomplexan parasite Plasmodium falciparum. Primary therapy with quinine or artemisinin derivatives is generally effective in controlling P. falciparum parasitemia, but mortality from CM other severe remains unacceptably high. Herein, we report design synthesis a novel carbon monoxide-releasing molecule (CO-RM; ALF492) that fully protects mice against experimental (ECM) ALI. ALF492...
Malaria-associated acute respiratory distress syndrome (MA-ARDS) is a deadly complication of malaria, and its pathophysiology insufficiently understood. Both in humans murine models, MA-ARDS characterized by marked pulmonary inflammation. We investigated the role hemozoin C57Bl/6 mice infected with Plasmodium berghei NK65, P. ANKA, chabaudi AS. By quantifying lungs measuring disease parameters MA-ARDS, we demonstrated highly significant correlation between concentrations, lung weights,...
γδ T cells play key nonredundant roles in immunity to infections and tumors. Thus, it is critical understand the molecular mechanisms responsible for cell activation expansion vivo. In striking contrast their αβ counterparts, costimulation requirements of remain poorly understood. Having previously described a role TNFR superfamily member CD27, we since screened other costimulatory receptors activation. We report this article that Ig receptor CD28 (but not its related protein ICOS) expressed...
Crystallographic studies of the hydrogenases (Hases) from Desulfovibrio gigas (Dg) and vulgaris Miyazaki (DvM) have revealed heterodinuclear nickel-iron active centers in both enzymes. The structures, which represent as-isolated (unready) Ni-A (S = (1)/(2)) enzyme state, disclose a nonprotein ligand (labeled as X) bridging two metals. atom was suggested to be an oxygenic (O(2)(-) or OH(-)) species Dg Hase inorganic sulfide DvM Hase. To determine nature chemical characteristics Ni-X-Fe Hase,...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXT57Fe Q-Band Pulsed ENDOR of the Hetero-Dinuclear Site Nickel Hydrogenase: Comparison NiA, NiB, and NiC StatesJ. E. Huyett, M. Carepo, A. Pamplona, R. Franco, I. Moura, J. G. B. HoffmanView Author Information Department Chemistry, Northwestern University Evanston, Illinois 60208-3113 Departamento de Química Centro Fina e Biotecnologia, Faculdade Ciências Tecnologia Universidade Nova Lisboa, 2825 Monte Caparica, Portugal Cite this: Am. Chem....
Cerebral malaria (CM) is a major cause of death due to Plasmodium infection. Both parasite and host factors contribute the onset CM, but precise cellular molecular mechanisms that its pathogenesis remain poorly characterized. Unlike conventional αβ-T cells, previous studies on murine γδ-T cells failed identify nonredundant role for this T cell subset in experimental cerebral (ECM). Here we show mice lacking are resistant ECM when infected with berghei ANKA sporozoites, liver-infective form...
Abstract Background Malaria pigment (haemozoin, Hz) has been the focus of diverse research efforts. However, identification Hz-containing leukocytes or parasitized erythrocytes is usually based on microscopy, with inherent limitations. Flow cytometric detection depolarized Side-Scatter more accurate and its adaptation to common bench top flow cytometers might allow several applications. These can range from ex-vivo in-vitro functional analysis Red-Blood-Cells (pRBCs) assess antimalarial...
OPINION article Front. Immunol., 06 December 2023Sec. Parasite Immunology Volume 14 - 2023 | https://doi.org/10.3389/fimmu.2023.1242306
Introdução: O desenvolvimento neurológico ocorre por meio de um processo contínuo com seu início no período neonatal e na infância, estendendo-se até a maturação das habilidades adquiridas. A educação em saúde é conjunto práticas que facilitam aprendizagem promovendo autonomia da comunidade apoia-se tecnologias auxiliam padronizam as orientações fornecidas visando facilitar promoção prevenção agravos intervenções educativas transmitem conhecimento aos indivíduos. Objetivo: realizar validação...