Miguel Muñoz‐Ruiz
A5013611309- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- COVID-19 Clinical Research Studies
- Vaccine Coverage and Hesitancy
- IL-33, ST2, and ILC Pathways
- Long-Term Effects of COVID-19
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- COVID-19 and healthcare impacts
- Hepatitis B Virus Studies
- Heart Rate Variability and Autonomic Control
- Technology in Education and Healthcare
- Immune responses and vaccinations
- Immune Response and Inflammation
- Botulinum Toxin and Related Neurological Disorders
- Peripheral Nerve Disorders
- Cytomegalovirus and herpesvirus research
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Pain Mechanisms and Treatments
- CRISPR and Genetic Engineering
Central Finland Health Care District
2021-2025
Research Institute Hospital 12 de Octubre
2011-2023
The Francis Crick Institute
2020-2023
King's College London
2022-2023
University of Lisbon
2016-2017
Universidad Complutense de Madrid
2009-2016
Centro de Investigación Biomédica en Red
2013
Improved understanding and management of COVID-19, a potentially life-threatening disease, could greatly reduce the threat posed by its etiologic agent, SARS-CoV-2. Toward this end, we have identified core peripheral blood immune signature across 63 hospital-treated patients with COVID-19 who were otherwise highly heterogeneous. The includes discrete changes in B myelomonocytic cell composition, profoundly altered T phenotypes, selective cytokine/chemokine upregulation SARS-CoV-2-specific...
The efficacy and safety profiles of vaccines against SARS-CoV-2 in patients with cancer is unknown. We aimed to assess the immunogenicity BNT162b2 (Pfizer-BioNTech) vaccine cancer.
Abstract The thymus is a primary lymphoid organ, essential for T cell maturation and selection. There has been long-standing interest in processes underpinning generation the potential to manipulate it clinically, because alterations of development or function can result severe immunodeficiency autoimmunity. Here, we identify epithelial-mesenchymal hybrid cells, capable long-term expansion vitro, able reconstitute an anatomic phenocopy native thymus, when combined with thymic interstitial...
ABSTRACT Background The efficacy and safety profile of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have not been definitively established in immunocompromised patient populations. Patients with a known cancer diagnosis were hitherto excluded from trials the currently clinical use. Methods This study presents data on immune BNT162b2 (Pfizer-BioNTech) vaccine 54 healthy controls 151 mostly elderly patients solid haematological malignancies, respectively,...
The increasing implication of lymphocytes in general physiology and immune surveillance outside infection poses the question how their antigen receptors might be involved. Here, we show that macromolecular aggregates intraepidermal γδ T cell (TCRs) mouse skin aligned with depended on Skint1, a butyrophilin-like (BTNL) protein expressed by differentiated keratinocytes (KCs) at steady state. Interruption TCR-mediated 'normality sensing' had no impact numbers but altered signature phenotype,...
A Correction to this paper has been published: https://doi.org/10.1038/s41591-020-01186-5
Abstract Person-to-person transmission of SARS-CoV-2 virus has triggered a global emergency because its potential to cause life-threatening Covid-19 disease. By comparison paucisymptomatic clearance by most individuals, been proposed reflect insufficient and/or pathologically exaggerated immune responses. Here we identify consensus peripheral blood signature across 63 hospital-treated patients who were otherwise highly heterogeneous. The core conspicuously blended adaptive B cell responses...
<h3>Abstract</h3> This most comprehensive analysis to date of γδ T cells in the murine uterus reveals them compose a unique local T-cell compartment. Consistent with earlier reports, expressed canonical Vγ6Vδ1 TCR, and produced interleukin (IL)-17A upon stimulation. Nonetheless, contrasting uterine were not obviously intraepithelial, being more akin sub-epithelial Vγ6Vδ1<sup>+</sup> at several other anatomical sites. By contrast tissues however, compartment also included non-Vγ6<sup>+</sup>,...
Abstract A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3 + CD4 CD8 − FoxP3 ), naïve CD44 int/low CD62L central memory high effector EMRA ). In each subset, a dual staining for Ki‐67 expression DNA content employed distinguish cycle phases: G 0 (Ki67 , with 2n DNA), 1 S‐G 2 /M < ≤ 4n). This established analysis of (C57BL/6J) spleen.
Diabetic sensorimotor peripheral neuropathy (DSPN) is a common complication of type 1 diabetes mellitus (T1DM). However, it still unclear how the cortical processing proprioceptive afference altered due to DSPN. Cortical responses right and left ankle joint rotations were recorded with magnetoencephalography pooled together in 20 T1DM participants healthy controls for source space comparisons. also underwent lower limb nerve-conduction study correlate nerve function responses. Primary (SM1)...
Whereas pathogen-specific T and B cells are a primary focus of interest during infectious disease, we have used COVID-19 to ask whether their emergence comes at cost broader cell repertoire disruption. We applied genomic DNA-based approach concurrently study the immunoglobulin-heavy (IGH) receptor (TCR) β δ chain loci 95 individuals. Our detected anticipated focusing for IGH repertoire, including expansions clusters related sequences temporally aligned with SARS-CoV-2–specific...
Elicitation of allergic contact dermatitis (ACD), an inflammatory type 4 hypersensitivity disease, induces skin infiltration by polyclonal effector CD8 αβ T cells and precursors tissue-resident memory (T
The ready availability of human blood makes it the first choice for immuno-monitoring. However, this has been largely confined to static metrics, particularly resting T cell phenotypes. Conversely, dynamic assessments have mostly relied on stimulation in vitro which is subject multiple variables. Here, immunodynamic insights from peripheral are shown be obtainable by applying a revised approach cell-cycle analysis. Specifically, refined flow cytometric protocols were employed, assuring...
The human αβ T-cell receptor (TCR) is composed of a variable heterodimer (TCRαβ) and three invariant dimers (CD3γε, CD3δε, ζζ/CD247 2 ). role each chain in the stepwise interactions among TCR chains along assembly still not fully understood. Despite high sequence homology between CD3γ CD3δ, clinical consequences corresponding immunodeficiencies (ID) humans are very different (mild severe, respectively), mouse models do recapitulate findings ID. To try to understand such disparities, we...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
The T cell antigen receptors (TCR) of αβ and γδ lymphocytes are believed to assemble in a similar fashion humans. Firstly, or TCR chains incorporate CD3δε dimer, then CD3γε dimer finally ζζ homodimer, resulting complexes with the same CD3 stoichiometry. Partial reduction expression highly homologous CD3γ CD3δ proteins would thus be expected have impact assembly surface both isotypes. To test this hypothesis, we compared primary cells from healthy donors carrying single null leaky mutation...
Immunodeficiencies of most T-cell receptor (TCR) components (TCRID) have been reported in almost 40 patients worldwide who also, at times, shown signs autoimmunity. We updated their clinical, immunological, and molecular features with an emphasis on practical diagnosis, as the range disorder grows complexity new partial defects. Cellular animal models are also reviewed some cases reveal limitations for predicting TCRID immunopathology.