A5004838915- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Malaria Research and Control
- Mosquito-borne diseases and control
- CAR-T cell therapy research
- Vector-borne infectious diseases
- Nanowire Synthesis and Applications
- Blood disorders and treatments
- Plant Genetic and Mutation Studies
- Subcritical and Supercritical Water Processes
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Force Microscopy Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Diabetes and associated disorders
- Pharmaceutical and Antibiotic Environmental Impacts
- Neurogenetic and Muscular Disorders Research
- Biochemical and Molecular Research
- Accounting and Financial Management
Research Institute Hospital 12 de Octubre
2018-2024
Centro Nacional de Biotecnología
2009-2020
University of La Salle
2018
Universidad Complutense de Madrid
2018
Corporación Universitaria del Huila
2016
University of Freiburg
2016
Universidad Autónoma de Madrid
2009-2013
Consejo Superior de Investigaciones Científicas
2007-2012
University of Milan
2011
Unidades Centrales Científico-Técnicas
2007
We describe humans with rare biallelic loss-of-function PTCRA variants impairing pre-α T cell receptor (pre-TCRα) expression. Low circulating naive αβ counts at birth persisted over time, normal memory and high γδ counts. Their TCRα repertoire was biased, which suggests that noncanonical thymic differentiation pathways can rescue development. Only a minority of these individuals were sick, infection, lymphoproliferation, and/or autoimmunity. also report 1 in 4000 from the Middle East South...
In the presence of Mn 2Y , an activity in a preparation purified Bacillus subtilis RecN degrades singlestranded (ss) DNA with 3' !5' polarity.This is not associated itself, because from cells lacking polynucleotide phosphorylase (PNPase) does show exonuclease activity.We here that, and low-level inorganic phosphate (P i ), PNPase ssDNA.The limited end-processing regulated by ATP inactive Mg or high-level P .In contrast, RNase requires suggesting that degradation RNA ssDNA occur mutually...
The Bacillus subtilis recH342 strain, which decreases interspecies recombination without significantly affecting the frequency of transformation with homogamic DNA, carried a point mutation in putative recX (yfhG) gene, and was renamed as recX342. We show that RecX (264 residues long), shares partial identity Proteobacterial (<180 residues), is genuine protein, its primary function to modulate SOS response facilitate RecA-mediated recombinational repair genetic recombination. RecX-YFP formed...
Bacillus subtilis pnpA gene product, polynucleotide phosphorylase (PNPase), is involved in double-strand break (DSB) repair via homologous recombination (HR) or non-homologous end-joining (NHEJ). RecN among the first responders to localize at DNA DSBs, with PNPase facilitating formation of a discrete focus per nucleoid. PNPase, which co-purifies RecA and RecN, was able degrade single-stranded (ss) 3′ → 5′ polarity presence Mn2+ low inorganic phosphate (Pi) concentration, extend 3′-OH end...
Genetic and cytological evidences suggest that Bacillus subtilis RecN acts prior to after end-processing of DNA double-strand ends via homologous recombination, appears participate in the assembly a repair centre interacts with incoming single-stranded (ss) during natural transformation. We have determined architecture RecN–ssDNA complexes by atomic force microscopy (AFM). ATP induces changes stimulates inter-complex assembly, thereby increasing local concentration ends. The large CII CIII...
A silicon nanowire-based biosensor has been designed and applied for label-free ultrasensitive detection of the early stage recombinational DNA repair by RecA protein. Silicon nanowires transistors were fabricated atomic force microscopy nanolithography integrated into a microfluidic environment. The sensor operates measuring changes in resistance nanowire as biomolecular reactions proceed. We show that nanoelectronic can detect differentiate several steps binding to single-stranded filament...
All organisms have developed a variety of DNA repair mechanisms to cope with double strands breaks (DSBs). In replicating cells, homologous recombination (HR), which uses an intact template restore lost information at the break site, is main pathway for error-free one- or two-ended DSBs and promoting re-establishment replication forks during vegetative growth. Genetic, cytological biochemical approaches were used analyse requirements exponentially growing Bacillus subtilis cells survive...
The population of malaria-causing parasites is characterized by great genetic diversity. Knowledge the polymorphism generation mechanism a central issue for developing effective vaccines against malaria and understanding parasite structure. Plasmodium vivax diversity has been explained in terms two major factors: natural selection intragenic recombination. A modular organization was found within P. Duffy binding protein present work. Four Colombian isolates have identical sequences to...
The human αβ T-cell receptor (TCR) is composed of a variable heterodimer (TCRαβ) and three invariant dimers (CD3γε, CD3δε, ζζ/CD247 2 ). role each chain in the stepwise interactions among TCR chains along assembly still not fully understood. Despite high sequence homology between CD3γ CD3δ, clinical consequences corresponding immunodeficiencies (ID) humans are very different (mild severe, respectively), mouse models do recapitulate findings ID. To try to understand such disparities, we...
The invariant TCR ζ/CD247 homodimer is crucial for TCR/CD3 expression and signaling through its 3 immunoreceptor tyrosine-based activation motifs (ITAMs). Homozygous null mutations in CD247 lead to immunodeficiency, while carriers exhibit 50% reduced surface CD3. It unclear whether of other variants show dominant-negative effects.
Transcription of the Salmonella enterica recA gene is negatively controlled by LexA protein, repressor SOS response. The introduction a mutation (recAo6869) in binding site, promoter region S. ATCC 14028 gene, allowed analysis effect that RecA protein overproduction has on fitness this virulent strain. orally but not intraperitoneally inoculated recAo6869 cells decreased dramatically. However, response mutant was induced normally, and there no increase sensitivity strain toward DNA-damaging...
UUbiquitous RarA AAA+ ATPases play crucial roles in the cellular response to blocked replication forks pro- and eukaryotes. Here, we provide evidence that absence of reduced viability recA, recO recF15 cells during unperturbed growth. The rarA gene was epistatic recO recF genes H2O2- or MMS-induced DNA damage. Conversely, inactivation partially suppressed HR defect mutants lacking end-resection (addAB, recJ, recQ, recS) branch migration (ruvAB, recG, radA) activity. contributes RecA...
Abstract: Functional and structural analyses of major histocompatibility complex (MHC) class I molecules the Aotus genus are necessary to validate it as a solid animal model for biomedical research. We thus isolated, cloned sequenced exons 2 3 from three species ( A. nancymaae , nigriceps vociferans ). found 24 sequences, which divided into two different groups (Ao‐g1 Ao‐g2). A further sequence was identified processed pseudogene (Aona‐PS2). Both evolution variability showed that Ao‐g1 Ao‐g2...
<title>Abstract</title> To create an exact copy of the entire genome, replication should be completed promptly and accurately. However, obstacles can arise during that cause genotoxic fork stalling. The DNA damage response pathway plays a crucial role in preventing collapse by stabilizing stalled fork, regulating repair, promoting restart. There are multiple pathways to recover forks, including double-strand break (DSB)-mediated recovery. SMARCAL1, damage-binding protein involved forks is...