Michaël Dussiot
A5071098590- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- T-cell and Retrovirus Studies
- Iron Metabolism and Disorders
- Blood transfusion and management
- Blood properties and coagulation
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Erythropoietin and Anemia Treatment
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- Viral-associated cancers and disorders
- Renal Diseases and Glomerulopathies
- Mast cells and histamine
- Immunodeficiency and Autoimmune Disorders
- Heat shock proteins research
- T-cell and B-cell Immunology
- Cellular transport and secretion
- Phagocytosis and Immune Regulation
- Pancreatic function and diabetes
- Angiogenesis and VEGF in Cancer
Institut des Maladies Génétiques Imagine
2016-2025
Université Paris Cité
2016-2025
Inserm
2015-2024
Laboratory of Excellence GR-Ex
2015-2024
Biologie Intégrée du Globule Rouge
2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2022-2024
Sorbonne Paris Cité
2011-2022
La Ligue Contre le Cancer
2022
Centre National de la Recherche Scientifique
2012-2021
Délégation Paris 5
2005-2021
As in human disease, macrophages (MØ) are central players the development and progression of experimental atherosclerosis. In this study we have evaluated phenotype MØ associated with atherosclerosis apolipoprotein E (ApoE) knockout (KO) mouse model.We found that bone marrow-derived submitted to M1 M2 polarization specifically expressed arginase (Arg) II Arg I, respectively. This distinct expression was used evaluate frequency distribution cross-sections atherosclerotic plaques ApoE KO mice....
Mass spectrometry-based proteomics now enables the absolute quantification of thousands proteins in individual cell types. We used this technology to analyze dynamic proteome changes occurring during human erythropoiesis. quantified expression 6,130 erythroid differentiation from late burst-forming units-erythroid (BFU-Es) orthochromatic erythroblasts. A modest correlation between mRNA and protein was observed. identified several with unexpected patterns cells, highlighting a breakpoint...
Microbial nucleic acid recognition serves as the major stimulus to an antiviral response, implying a requirement limit misrepresentation of self acids non-self and induction autoinflammation. By systematic screening using panel interferon-stimulated genes we identify two siblings singleton variably demonstrating severe neonatal anemia, membranoproliferative glomerulonephritis, liver fibrosis, deforming arthropathy increased anti-DNA antibodies. In both families biallelic mutations in DNASE2,...
Enteropathy-associated T-cell lymphoma (EATL) is a rare but severe complication of coeliac disease (CeD), often preceded by low-grade clonal intraepithelial lymphoproliferation, referred to as type II refractory CeD (RCDII). Knowledge on underlying oncogenic mechanisms remains scarce. Here, we analysed and compared the mutational landscape RCDII EATL in order identify genetic drivers CeD-associated lymphomagenesis.Pure populations RCDII-cells derived from intestinal biopsies (n=9) or sorted...
We describe humans with rare biallelic loss-of-function PTCRA variants impairing pre-α T cell receptor (pre-TCRα) expression. Low circulating naive αβ counts at birth persisted over time, normal memory and high γδ counts. Their TCRα repertoire was biased, which suggests that noncanonical thymic differentiation pathways can rescue development. Only a minority of these individuals were sick, infection, lymphoproliferation, and/or autoimmunity. also report 1 in 4000 from the Middle East South...
BACKGROUND Storage lesion may explain the rapid clearance of up to 25% transfused red blood cells (RBCs) in recipients. Several alterations affect stored RBC but a quantitative, whole cell–based predictor transfusion yield is lacking. Because RBCs with reduced surface area are retained by spleen, we quantified changes dimensions during storage. STUDY DESIGN AND METHODS Using imaging flow cytometry observed dimension and morphology upon storage, along that conventional biochemical mechanical...
In systemic lupus erythematosus (SLE), autoantibody production can lead to kidney damage and failure, known as nephritis. Basophils amplify the synthesis of autoantibodies by accumulating in secondary lymphoid organs. Here, we show a role for prostaglandin D
Patients with high-risk, nonmuscle-invasive bladder cancer (NMIBC) frequently relapse after standard intravesical bacillus Calmette-Guérin (BCG) therapy and may have a dismal outcome. The mechanisms of resistance to such immunotherapy remain poorly understood. Here, using cell lines, freshly resected human tumors, samples from cohorts patients before BCG therapy, we demonstrate 2 distinct patterns immune subversion upon relapse. In the first pattern, intracellular infection cells induced...
Objective— In addition to its role in the clearance of haptoglobin-hemoglobin (Hp-Hb) complexes, CD163 is a macrophage scavenger receptor for tumor necrosis factor–like weak inducer apoptosis (TWEAK). We recently reported that CD163/TWEAK plasma ratio could be potential biomarker atherothrombosis asymptomatic subjects. this study, we assessed soluble TWEAK (sTWEAK) and (sCD163) levels white males with peripheral artery disease (PAD) atherothrombotic femoral plaques evaluate their...
While ineffective erythropoiesis has long been recognized as a key contributor to anemia in thalassemia, its role of sickle cell disease (SCD) not critically explored. Using vitro and vivo derived human erythroblasts we assessed the extent SCD. Modeling bone marrow hypoxic environment, found that hypoxia induces death starting at polychromatic stage, positively selecting cells with high levels fetal hemoglobin (HbF). Cell was associated cytoplasmic sequestration heat shock protein 70 rescued...
Macrophages, key regulators of healing/regeneration processes, strongly infiltrate ischemic tissues from patients suffering critical limb ischemia (CLI). However pro-inflammatory markers correlate with disease progression and risk amputation, suggesting that modulating macrophage activation state might be beneficial. We previously reported thrombospondin-1 (TSP-1) is highly expressed in during CLI humans. TSP-1 a matricellular protein displays well-known angiostatic properties cancer,...
Abstract Spleen dysfunction is central to morbidity and mortality in children with sickle cell anemia (SCA). The initiation determinants of spleen injury, including acute splenic sequestration (ASS) have not been established. We investigated function longitudinally a cohort 57 infants SCA enrolled at 3 6 months age followed up 24 explored the respective contribution decreased red blood (RBC) deformability increased RBC adhesion on ASS. was evaluated by sequential 99mTc heated scintigraphy...
Extranodal NK/T-cell lymphoma (ENKTCL) is an Epstein-Barr virus (EBV)-related neoplasm with male dominance and a poor prognosis. A better understanding of the genetic alterations their functional roles in ENKTCL could help improve patient stratification treatments. In this study, we performed comprehensive analysis 178 cases to delineate landscape mutations, copy number (CNA), structural variations, identifying 34 driver genes including six previously unappreciated ones, namely, HLA-B,...
Although refrigerated storage slows the metabolism of volunteer donor RBCs, which is essential in transfusion medicine, cellular aging still occurs throughout this vitro process. Storage-induced microerythrocytes (SMEs) are morphologically-altered senescent RBCs that accumulate during and cleared from circulation following transfusion. However, molecular alterations trigger clearance RBC subset remain to be identified. Using a staining protocol sorts long-stored SMEs (i.e., CFSEhigh)...