A5088404286- Mast cells and histamine
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Urticaria and Related Conditions
- Allergic Rhinitis and Sensitization
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Immune cells in cancer
- Chronic Lymphocytic Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Cell death mechanisms and regulation
- Diabetes and associated disorders
Tokyo Medical and Dental University
2015-2024
The University of Tokyo
1991-2024
Institute of Science Tokyo
2024
Advanced Research Institute
2020-2023
Japan Science and Technology Agency
2011-2015
Pediatrics and Genetics
2011
Tokyo University of Science
2006
Keio University
2004-2006
Kyoto University
2004
Tokyo Metropolitan Institute of Medical Science
1990-2003
Caspases are essential components of the mammalian cell death machinery. Here we test hypothesis that Caspase 9 (Casp9) is a critical upstream activator caspases through gene targeting in mice. The majority Casp9 knockout mice die perinatally with markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development. deletion prevents activation Casp3 embryonic brains vivo, Casp9-deficient thymocytes show resistance to subset apoptotic stimuli, including absence...
Abstract Mouse cell lines of different lineages have been established which constitutively secrete large quantities recombinant mouse interleukins (mIL2, mIL3, mIL4 or mIL5). An existing bovine papilloma virus‐based expression vector, pBV‐lMTHA, was modified to allow transformed X63Ag8‐653 myeloma cells, NIH 3T3 fibroblasts and C127 mammary tumor cells stably carry multiple copies the express inserted cDNA encoding a single interleukin constitutively, in high quantities. Cell with mIL2...
A poorly immunogenic murine colon cancer was used to investigate mechanisms of antitumor immunity. Injection tumor cells engineered by gene transfection secrete IL-2 stimulated an MHC class I-restricted cytolytic T lymphocyte (CTL) response against the parental tumor. The secreting produced in vivo, even absence CD4+ cells. Animals immunized with were protected subsequent challenge cell line. Similar findings demonstrated for other types. Thus, provision a helper lymphokine paracrine fashion...
The generation of antigen-specific antitumor immunity is the ultimate goal in cancer immunotherapy. When cells from a spontaneously arising murine renal cell tumor were engineered to secrete large doses interleukin-4 (IL-4) locally, they rejected predominantly T cell-independent manner. However, animals that IL-4-transfected tumors developed cell-dependent systemic parental tumor. This was tumor-specific and primarily mediated by CD8 + cells. Established could be cured immune response...
The murine pre-B cell-specific genes VpreB and lambda 5, as well the gene for mu heavy chain, were introduced into Ltk- fibroblast cells which normally do not express these genes. Stable transfectants carrying produced corresponding proteins of 15.5, 21.5, 75 kD. They secreted three a triple complex that could be immunoprecipitated by chain-specific antibodies, consisting one VpreB, chain. chain 5 disulfide-bonded with each other, while protein was noncovalently associated. These experiments...
Ticks are ectoparasitic arthropods that can transmit a variety of microorganisms to humans and animals during blood feeding, causing serious infectious disorders, including Lyme disease. Acaricides pharmacologic agents kill ticks. The emergence acaricide-resistant ticks calls for alternative control strategies tick-borne diseases. Many develop resistance after repeated infestations, but the nature this acquired anti-tick immunity remains poorly understood. Here we investigated cellular...
Anaphylaxis is a life-threatening hyperacute immediate hypersensitivity reaction. Classically, it depends on IgE, FcεRI, mast cells, and histamine. However, anaphylaxis can also be induced by IgG antibodies, an IgG1-induced passive type of systemic has been reported to depend basophils. In addition, was found that neither cells nor basophils were required in mouse models active anaphylaxis. Therefore, we investigated what receptors, are involved mice. We FcγRIIIA FcγRIV, platelet-activating...
The current paradigm is that inflammatory pain passively resolves following the cessation of inflammation. Yet, in a substantial proportion patients with diseases, resolution inflammation not sufficient to resolve pain, resulting chronic pain. Mechanistic insight into how resolved lacking. Here, we show macrophages actively control remotely from site by transferring mitochondria sensory neurons. During mice, M2-like infiltrate dorsal root ganglia contain somata neurons, concurrent recovery...
Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by anti-nuclear autoantibodies whose production promoted autoreactive T follicular helper (TFH) cells. During SLE pathogenesis, basophils accumulate in secondary lymphoid organs (SLO), amplify autoantibody and progression through mechanisms that remain to be defined. Here, we provide evidence for a direct functional relationship between TFH cells during both humans mice. PD-L1 upregulation on IL-4 are...
The interleukin 7 receptor (IL-7R) plays a crucial role in early B- and T-cell development. It consists of unique chain common gamma [IL-2 (IL-2Rgamma)]. Gene inactivation IL-7, IL-7R, IL-2Rgamma resulted severe impairment B T lymphopoiesis mice. In addition, IL-2Rgamma-deficient mice lack gammadelta cells the skin have impaired development natural killer (NK) intraepithelial lymphocytes. To explore IL-7/IL-7R system T- NK-cell development, we generated analyzed IL-7R-deficient were absent...
The analysis of the expression a chain IL-2 receptor (CD25.TAC) on surface B lineage cells In mouse bone marrow reveals that it is useful marker to distinguish pre-B-I from pre-B-II cells. CD25 Is not expressed CD45R(B220)+ c-kit+ CD43+ TdT+ λ5+ Cμ− slg− lgH locus DJH-rearranged marrow. It large cycling sig− and small resting CD43− sig- IgH VHDJH-rearranged Therefore, transition marked by downregulation c-kit terminal deoxynucleotldyl transferase (TdT), upregulatton CD25. SCID, RAG-2T, μMT...