A5068360947- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Melanoma and MAPK Pathways
- RNA Interference and Gene Delivery
- Synthesis and biological activity
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Cancer Mechanisms and Therapy
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- HER2/EGFR in Cancer Research
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Hemophilia Treatment and Research
- SARS-CoV-2 and COVID-19 Research
- Wnt/β-catenin signaling in development and cancer
- Circular RNAs in diseases
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
Icahn School of Medicine at Mount Sinai
2016-2025
Tisch Hospital
2015-2024
Mount Sinai Health System
2021-2023
Tisch Cancer Institute
2015-2022
Mount Sinai Hospital
2010-2019
Dana-Farber Cancer Institute
2019
Child Health and Development Institute
2017-2018
NYU Langone Health
2015
Pediatrics and Genetics
2015
Eastern Cooperative Oncology Group
2015
PD-1 immune checkpoint inhibitors have produced encouraging results in patients with hepatocellular carcinoma (HCC). However, what determines resistance to anti-PD-1 therapies is unclear. We created a novel genetically engineered mouse model of HCC that enables interrogation how different genetic alterations affect surveillance and response immunotherapies. Expression exogenous antigens MYC;Trp53 -/- HCCs led T cell-mediated surveillance, which was accompanied by decreased tumor formation...
Deep sequencing of small RNAs (sRNA-seq) is now the gold standard for RNA profiling and discovery. Biases in sRNA-seq have been reported, but their etiology remains unidentified. Through a comprehensive series experiments, we establish that predominant cause bias ligases. We further demonstrate ligases strong sequence-specific biases which distort profiles considerably. devised pooled adapter strategy to overcome this bias, validated method through data derived from microarray qPCR. In light...
Ovarian cancer is a leading cause of deaths among women. Effective targets to treat advanced epithelial ovarian (EOC) and biomarkers predict treatment response are still lacking because the complexity pathways involved in progression. Here we show that miR-181a promotes TGF-β-mediated epithelial-to-mesenchymal transition via repression its functional target, Smad7. phosphorylated Smad2 enriched recurrent compared with matched-primary tumours their expression associated shorter time...
Abstract Neutrophils are implicated in multiple homeostatic and pathological processes, but whether functional diversity requires discrete neutrophil subsets is not known. Here, we apply single-cell RNA sequencing to neutrophils from normal inflamed mouse tissues. Whereas conventional clustering yields alternative organizational structures, diffusion mapping plus velocity discloses a single developmental spectrum, ordered chronologically. Termed here neutrotime, this spectrum extends...
CD8+ cytotoxic T cells are critical for viral clearance from the lungs upon influenza virus infection. The contribution of antigen cross-presentation by DCs to induction anti-viral remains controversial. Here, we used a recombinant expressing nonstructural 1–GFP (NS1-GFP) reporter gene visualize route presentation lung infection in mice. We found that CD103+ were only subset carried intact GFP protein draining LNs. Strikingly, migratory not productively infected and thus able induce...
Microglia, the brain resident macrophages, critically shape forebrain neuronal circuits. However, their precise function in cerebellum is unknown. Here we show that human and mouse cerebellar microglia express a unique molecular program distinct from microglia. Cerebellar microglial identity was driven by CSF-1R ligand CSF-1, independently of alternate ligand, IL-34. Accordingly, CSF-1 depletion Nestin+ cells led to severe transcriptional alterations microglia, while remained intact....