Christoph Muus
A5003686433- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- IL-33, ST2, and ILC Pathways
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- interferon and immune responses
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Nanoparticle-Based Drug Delivery
- Immune responses and vaccinations
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Social Media in Health Education
- Nanopore and Nanochannel Transport Studies
- Supramolecular Self-Assembly in Materials
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
Broad Institute
2019-2023
Harvard University
2019-2023
Massachusetts Institute of Technology
2012-2022
Heidelberg University
2012
There is pressing urgency to understand the pathogenesis of severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2), which causes disease COVID-19. SARS-CoV-2 spike (S) protein binds angiotensin-converting enzyme (ACE2), and in concert with host proteases, principally transmembrane serine protease (TMPRSS2), promotes cellular entry. The cell subsets targeted by tissues factors that regulate ACE2 expression remain unknown. Here, we leverage human, non-human primate, mouse...
Targeted delivery of therapeutics to tumor neovasculature is potentially a powerful approach for selective cancer treatment. Integrins are heterodimeric transmembrane proteins involved in cell adhesion and signaling, their expression commonly upregulated cancers inflammatory diseases. The αvβ3 integrin differentially on angiogenic endothelial cells as well many cells. Here we demonstrate the differential targeting cisplatin prodrug-encapsulated poly(d,l-lactic-co-glycolic...
Abstract Neutrophils are implicated in multiple homeostatic and pathological processes, but whether functional diversity requires discrete neutrophil subsets is not known. Here, we apply single-cell RNA sequencing to neutrophils from normal inflamed mouse tissues. Whereas conventional clustering yields alternative organizational structures, diffusion mapping plus velocity discloses a single developmental spectrum, ordered chronologically. Termed here neutrotime, this spectrum extends...
Human erythropoiesis serves as a paradigm of physiologic cellular differentiation. This process is also considerable interest for better understanding anemias and identifying new therapies. Here, we apply deep transcriptomic accessible chromatin profiling to characterize faithful ex vivo human erythroid differentiation system from hematopoietic stem progenitor cells. We reveal stage-specific transcriptional states accessibility during various stages erythropoiesis, including 14,260...
There is pressing urgency to better understand the pathogenesis of severe acute respiratory syndrome (SARS) coronavirus (CoV) clade SARS-CoV-2. SARS-CoV-2, like SARS-CoV, utilizes ACE2 bind host cells. While initial SARS-CoV-2 cell entry and infection depend on in concert with protease TMPRSS2 for spike (S) protein activation, specific subsets targeted by tissues, factors that regulate expression, remain unknown. Here, we leverage human non-human primate (NHP) single-cell RNA-sequencing...
Abstract The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune cellular responses associated with COVID-19 infection, symptoms, lethality. To address this, we collected tissues from 11 organs during clinical autopsy of 17 individuals who succumbed COVID-19, a bank approximately 420 specimens. We generated...
Abstract Single-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) has emerged as a powerful tool dissecting regulatory landscapes and cellular heterogeneity. However, an exploration of systemic biases among scATAC-seq technologies remained absent. In this study, we benchmark the performance eight methods across 47 experiments using human peripheral blood mononuclear cells (PBMCs) reference sample develop PUMATAC, universal preprocessing pipeline, to handle various...
We report a simple and efficient way to accomplish the chemical modification of glass nanopores by means lipid self-assembly. Lipid coating improves success rate these as biosensors detect λ-DNA.
Single-cell RNA sequencing (scRNA-seq) is a powerful technique for describing cell states. Identifying the spatial arrangement of these states in tissues remains challenging, with existing methods requiring niche methodologies and expertise. Here, we describe segmentation by exogenous perfusion (SEEP), rapid integrated method to link surface proximity environment accessibility transcriptional identity within three-dimensional (3D) disease models. The utilizes steady-state diffusion kinetics...
The human airway contains specialized rare epithelial cells whose roles in respiratory disease are not well understood. Ionocytes express the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), while chemosensory tuft asthma-associated alarmins. However, surprisingly, exceedingly few mature have been identified lung cell atlases despite ready identification of ionocytes and neuroendocrine cells. To identify progenitors define their lineage relationship to cells, we generated a deep...
Coronavirus disease 2019 (COVID-19) is a global pandemic caused by novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). SARS-CoV-2 infection of host cells occurs predominantly via binding the viral surface spike protein to human angiotensin-converting enzyme 2 (ACE2) receptor. 1 Hypertension and pre-existing cardiovascular are risk factors for morbidity from COVID-19, it remains uncertain whether use angiotensin converting inhibitors (ACEi) or receptor blockers (ARB) impacts...