Sarah Warren
A5082913203- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- HER2/EGFR in Cancer Research
- Single-cell and spatial transcriptomics
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Radiomics and Machine Learning in Medical Imaging
- Breast Cancer Treatment Studies
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Melanoma and MAPK Pathways
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
Nanostring Technologies (United States)
2016-2025
Gilead Sciences (United States)
2023-2024
Institut Gustave Roussy
2021-2023
Inserm
2023
Kite (United States)
2023
Southampton Children's Hospital
2022
Seattle University
2010-2022
British Veterinary Association
2021
North Seattle College
2019
American Speech Language Hearing Association
2019
The mammalian innate immune system uses Toll-like receptors (TLRs) and Nod-LRRs (NLRs) to detect microbial components during infection. Often these molecules work in concert; for example, the TLRs can stimulate production of proforms cytokines IL-1beta IL-18, whereas certain NLRs trigger their subsequent proteolytic processing via caspase 1. Gram-negative bacteria use type III secretion systems (T3SS) deliver virulence factors cytosol host cells, where they modulate cell physiology favor...
Assays of the abundance immune cell populations in tumor microenvironment promise to inform oncology research and choice immunotherapy for individual patients. We propose measure intratumoral various with gene expression. In contrast IHC flow cytometry, expression assays yield high information content from a clinically practical workflow. Previous studies purified cells have reported hundreds genes showing enrichment single type, but utility these samples is unknown. use co-expression...
The Tumor Inflammation Signature (TIS) is an investigational use only (IUO) 18-gene signature that measures a pre-existing but suppressed adaptive immune response within tumors. TIS has been shown to enrich for patients who respond the anti-PD1 agent pembrolizumab. To explore this phenotype and across tumor types, we applied algorithm over 9000 gene expression profiles downloaded from Cancer Genome Atlas (TCGA). As expected based on prior evidence, tumors with known clinical sensitivity...
•Glycolytic index in melanoma negatively correlates with response to anti-PD1 therapy•Blocking lactate transport or knock out of glycolytic genes improves checkpoint therapy•Diclofenac blocks the transporters MCT1 and MCT4 a COX-independent manner•Inhibition glycolysis by MCT blockade does not impede T cell function SummaryTumor-derived lactic acid inhibits natural killer (NK) and, thereby, tumor immunosurveillance. Here, we report that patients high expression glycolysis-related show worse...
The relationship of SARS-CoV-2 pulmonary infection and severity disease is not fully understood. Here we show analysis autopsy specimens from 24 patients who succumbed to using a combination different RNA protein analytical platforms characterize inter-patient intra-patient heterogeneity virus infection. There spectrum high low cases associated with duration disease. High viral have activation interferon pathway genes predominant M1-like macrophage infiltrate. Low are more heterogeneous...
Patients with rheumatoid arthritis (RA) receive highly targeted biologic therapies without previous knowledge of target expression levels in the diseased tissue. Approximately 40% patients do not respond to individual and 5–20% are refractory all. In a biopsy-based, precision-medicine, randomized clinical trial RA (R4RA; n = 164), low/absent synovial B cell molecular signature had lower response rituximab (anti-CD20 monoclonal antibody) compared that tocilizumab (anti-IL6R although exact...
Abstract In less than nine months, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) killed over a million people, including >25,000 in New York City (NYC) alone. The COVID-19 pandemic caused by SARS-CoV-2 highlights clinical needs to detect infection, track strain evolution, and identify biomarkers of disease course. To address these challenges, we designed fast (30-minute) colorimetric test (LAMP) for infection from naso/oropharyngeal swabs large-scale shotgun...
Acute myeloid leukemia (AML) is a molecularly and clinically heterogeneous hematological malignancy. Although immunotherapy may be an attractive modality to exploit in patients with AML, the ability predict groups of types cancer that will respond immune targeting remains limited. This study dissected complexity architecture AML at high resolution assessed its influence on therapeutic response. Using 442 primary bone marrow samples from three independent cohorts children adults we defined...
Abstract Developing strategies to inflame tumors is critical for increasing response immunotherapy. Here, we report that low-dose radiotherapy (LDRT) of murine promotes T-cell infiltration and enables responsiveness combinatorial immunotherapy in an IFN-dependent manner. Treatment efficacy relied upon mobilizing both adaptive innate immunity depended on cytotoxic CD4+ CD8+ T cells. LDRT elicited predominantly cells with features exhausted effector cells, a subset expressing NKG2D exhibiting...
Abstract Listeria monocytogenes escapes from the phagosome of macrophages and replicates within cytosolic compartment. The macrophage responds to L. through detection pathways located on cell surface (TLRs) cytosol (Nod-like receptors) promote inflammatory processes aimed at clearing pathogen. Cytosolic activates caspase 1, resulting in post-translational processing cytokines IL-1β IL-18 as well 1-dependent death (pyroptosis). We demonstrate that presence compartment induces 1 activation...
Protein expression in formalin-fixed, paraffin-embedded tissue is routinely measured by IHC or quantitative fluorescence (QIF) on a handful of markers single section. Digital spatial profiling (DSP) allows spatially informed simultaneous assessment multiple biomarkers. Here we demonstrate the DSP technology using 44-plex antibody cocktail to find protein that could potentially be used predict response immune therapy melanoma.Experimental Design: The NanoString GeoMx compared with automated...
Abstract Pathogens are detected by pattern recognition receptors that, upon activation, orchestrate an appropriate immune response. The TLRs and the nucleotide-binding oligomerization domain-like (NLRs) prototypic that detect extracellular cytosolic pathogens, respectively. Listeria monocytogenes has both phases is in cytosol members of NLR family. These include two members, NLRC4 NLRP3, detection L. monocytogenes, induce assembly inflammasome. Inflammasomes serve as platforms for activation...
ABSTRACT Inflammasomes are cytosolic multiprotein complexes that assemble in response to infectious or noxious stimuli and activate the CASPASE-1 protease. The inflammasome containing nucleotide binding domain-leucine-rich repeat (NBD-LRR) protein NLRC4 (interleukin-converting enzyme protease-activating factor [IPAF]) responds presence of bacterial proteins such as flagellin inner rod component type III secretion systems (e.g., Salmonella PrgJ). In some instances, infection with Legionella...
Abstract Background The 18-gene tumor inflammation signature (TIS) is a clinical research assay that enriches for benefit to immune checkpoint blockade. We evaluated its ability predict of immunotherapy in cancer patients treated with PD-1 inhibitors routine care. Methods CERTIM cohort prospective which includes receiving Cochin University hospital. RNA extracted from 58 archival formalin fixed paraffin embedded blocks (including 38 lung cancers, 5 melanomas, 10 renal carcinomas, 4...