A5101812429- Cancer Research and Treatments
- Immune Cell Function and Interaction
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Single-cell and spatial transcriptomics
- Respiratory viral infections research
- Glioma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Multiple Myeloma Research and Treatments
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Heart Rate Variability and Autonomic Control
- Cell Image Analysis Techniques
- Photodynamic Therapy Research Studies
- Spinal Dysraphism and Malformations
- Wnt/β-catenin signaling in development and cancer
- Parkinson's Disease and Spinal Disorders
- Phagocytosis and Immune Regulation
- Chromatin Remodeling and Cancer
- Hedgehog Signaling Pathway Studies
- Macrophage Migration Inhibitory Factor
Peking University
2024
Nanostring Technologies (United States)
2017-2023
North Seattle College
2021
The University of Texas Health Science Center at San Antonio
2021
Seattle University
2017
The Military General Hospital of Beijing PLA
2014
The relationship of SARS-CoV-2 pulmonary infection and severity disease is not fully understood. Here we show analysis autopsy specimens from 24 patients who succumbed to using a combination different RNA protein analytical platforms characterize inter-patient intra-patient heterogeneity virus infection. There spectrum high low cases associated with duration disease. High viral have activation interferon pathway genes predominant M1-like macrophage infiltrate. Low are more heterogeneous...
Breast cancer is a heterogenous disease with variability in tumor cells and the surrounding microenvironment (TME). Understanding molecular diversity breast critical for improving prediction of therapeutic response prognostication. High-plex spatial profiling tumors enables characterization heterogeneity TME, which can holistically illuminate biology growth, dissemination and, ultimately, to therapy. The GeoMx Digital Spatial Profiler (DSP) researchers spatially resolve quantify proteins RNA...
Entry of antigen-specific T cells into human tumors is critical for immunotherapy, but the underlying mechanisms are poorly understood. Here, we combined high-dimensional spatial analyses with in vitro and vivo modeling to study immune infiltration multiple myeloma (MM) its precursor monoclonal gammopathy undetermined significance (MGUS). Clustered tumor growth was a feature MM not MGUS biopsies, this pattern reproduced humanized mouse models. biopsies exhibited intralesional as well...
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Pancreatic ductal adenocarcinoma (PDAC) has dismal 5-year survival (<9%). We hypothesize that exposure of tumors to conventional therapies may preferentially modulate immune biomarkers in the tumor microenvironment PDAC. PDAC patients who underwent upfront surgical resection or received neoadjuvant FOLFIRINOX with without radiotherapy followed by were selected for study. Total expression immunologically relevant transcripts and spatially resolved proteins was quantitated using multiplexed...
Abstract Medulloblastoma (MB) is the most common primary malignant pediatric brain cancer. We recently identified novel roles for MEK/MAPK pathway in regulating human Sonic Hedgehog (SHH) MB tumorigenesis. The MEK inhibitor, selumetinib, decreased SHH growth while extending survival mouse models. However, treated mice ultimately succumbed to disease progression. Here, we perform RNA sequencing on selumetinib-treated orthotopic xenografts identify molecular pathways that compensate inhibition...
ABSTRACT The relationship of SARS-CoV-2 lung infection and severity pulmonary disease is not fully understood. We analyzed autopsy specimens from 24 patients who succumbed to using a combination different RNA protein analytical platforms characterize inter- intra-patient heterogeneity virus infection. There was spectrum high low cases that associated with duration activation interferon pathway genes. Using digital spatial profiling platform, the corresponded distinct expression response...
2-Methoxyestradiol (2-ME2) possesses anti-tumorigenic activities in multiple tumor models with acceptable tolerability profile humans. Incomplete understanding of the mechanism has hindered its development as an compound. We have identified for first-time macrophage stimulatory protein 1 receptor (MST1R) a potential target 2-ME2 prostate cancer cells. Human tissue validation studies show that MST1R (a.k.a RON) levels are significantly elevated tissues compared to adjacent normal/benign...
Introduction. Glioblastoma (GBM) is the most common primary adult brain tumour with a median overall survival (OS) of 12–15 months. Molecular characterization multiple immunooncology targets in GBM may help target novel immunotherapeutic strategies. We used NanoString GeoMx® Digital Spatial Profiling (DSP) to assess protein methylated versus unmethylated IDH-wild-type glioblastoma. Methods. DSP technology uses antibodies conjugated indexing DNA oligos UV photocleavable linker. Tissue regions...
To explore the link between leucoaraiosis and recovery of neurological function in elderly patients with acute cerebral infarction.The study included patients, hospitalized infarction. Magnetic resonance imaging examinations were performed before or within 5 days after admission; T1-weighted, T2-weighted, fluid-attenuated inversion diffusion-weighted sequence data collected. Using Fazekas scale, (white matter hyperintensity [WMH]) was graded as 0 (absent), 1 (mild), 2 (moderate) 3 (severe)...
Abstract Cyclic dinucleotide (CDN) agonists of stimulator interferon genes (STING) activate innate immunity to increase tumor immunogenicity. However, the efficacy CDNs is limited by drug delivery barriers, including inefficient transport cytosol where STING localized. We recently developed STING-activating nanoparticles (STING-NPs), polymer vesicles designed for enhanced cytosolic endogenous CDN ligand STING, 2’3’ cyclic guanosine monophosphate-adenosine monophosphate (cGAMP)....
Abstract Breast cancers are complex cellular ecosystems where heterotypic interactions play central roles in disease progression and response to therapy. However, our knowledge of the composition organization breast cancer remains limited. We present a comprehensive single cell spatially resolved transcriptomic atlas human cancers. The 10X Genomics Chromium platform was used generate data (scRNA-Seq) from more than 120,000 cells sampled 26 CITE-Seq employed simultaneously protein...
Abstract Muscle-invasive bladder cancer (MIBC) is an aggressive disease with limited therapeutic options. PD-1 pathway targeting immunotherapies have been approved to treat advanced cancer, but most patients exhibit primary resistance, suggesting that immune evasion mechanisms exist. The PPARγ has identified as a potential target in MIBC associated reduced CD8+ T-cell infiltration and increased resistance immunotherapies. We comprehensively profiled the tumor microenvironment (TME)...
Abstract Although many new drugs have been approved and more clinical trials are being conducted for various disease therapies in recent decades, efficient identification of reliable therapeutic targets is still a big challenge during drug development. Therefore, tremendous efforts put into advancing the technology to quickly determine altered biomarkers samples. Nanostring nCounter GeoMxTM Digital Spatial Profiling (GeoMx DSP) has used multiple projects exhibited its outstanding performance...