David Lieb
A5060516869- Cancer Immunotherapy and Biomarkers
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Renal Diseases and Glomerulopathies
- Single-cell and spatial transcriptomics
- Atherosclerosis and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Advanced Chemical Sensor Technologies
- Transportation and Mobility Innovations
- interferon and immune responses
- Long-Term Effects of COVID-19
- Chronic Kidney Disease and Diabetes
- Cytomegalovirus and herpesvirus research
- Advanced Vision and Imaging
- Robotics and Sensor-Based Localization
- Ferroptosis and cancer prognosis
- Pancreatic and Hepatic Oncology Research
- Lymphoma Diagnosis and Treatment
- Video Surveillance and Tracking Methods
Broad Institute
2017-2024
Massachusetts Institute of Technology
2018-2024
Hofstra University
2024
Feinstein Institute for Medical Research
2022
Harvard University
2018-2021
Stanford University
2005-2007
Thermedical (United States)
1993
Immune responses to cancer are highly variable, with mismatch repair-deficient (MMRd) tumors exhibiting more anti-tumor immunity than repair-proficient (MMRp) tumors. To understand the rules governing these varied responses, we transcriptionally profiled 371,223 cells from colorectal and adjacent normal tissues of 28 MMRp 34 MMRd individuals. Analysis 88 cell subsets their 204 associated gene expression programs revealed extensive transcriptional spatial remodeling across discover hubs...
Mechanisms underlying severe coronavirus disease 2019 (COVID-19) remain poorly understood. We analyze several thousand plasma proteins longitudinally in 306 COVID-19 patients and 78 symptomatic controls, uncovering immune non-immune linked to COVID-19. Deconvolution of our proteome data using published scRNA-seq datasets reveals contributions from circulating tissue cells. Sixteen percent display reduced inflammation yet comparably poor outcomes. Comparison who died severely ill survivors...
The relationship of SARS-CoV-2 pulmonary infection and severity disease is not fully understood. Here we show analysis autopsy specimens from 24 patients who succumbed to using a combination different RNA protein analytical platforms characterize inter-patient intra-patient heterogeneity virus infection. There spectrum high low cases associated with duration disease. High viral have activation interferon pathway genes predominant M1-like macrophage infiltrate. Low are more heterogeneous...
Abstract While BRAF inhibitor combinations with EGFR and/or MEK inhibitors have improved clinical efficacy in V600E colorectal cancer (CRC), response rates remain low and lack durability. Preclinical data suggest that BRAF/MAPK pathway inhibition may augment the tumor immune response. We performed a proof-of-concept single-arm phase 2 trial of combined PD-1, sparatlizumab (PDR001), dabrafenib trametinib 37 patients CRC. The primary end point was overall rate, secondary points were...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDescription of the thermal energy analyzer (TEA) for trace determination volatile and nonvolatile N-nitroso compoundsDavid H. Fine, Firooz. Rufeh, David. Lieb, David P. RounbehlerCite this: Anal. Chem. 1975, 47, 7, 1188–1191Publication Date (Print):June 1, 1975Publication History Published online1 May 2002Published inissue 1 June 1975https://pubs.acs.org/doi/10.1021/ac60357a073https://doi.org/10.1021/ac60357a073research-articleACS...
Detailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage. The Accelerating Medicines Partnership RA/SLE Network aims to deconstruct autoimmune by examining within target tissues through multiple high-dimensional assays. Robust standardized protocols need be developed before at a single cell level can effectively compared across patient samples.Multiple clinical sites collected...
Abstract Systemic inflammation is central to aging‐related conditions. However, the intrinsic factors that induce are not well understood. We previously identified a cell‐autonomous pathway through which damaged nuclear DNA trafficked cytosol where it activates innate cytosolic sensors trigger inflammation. These results led us hypothesize released after cumulative damage contributes persistent in aging cells similar mechanism. Consistent with this notion, we found older harbored higher...
Although immune checkpoint inhibitors (ICIs), such as anti-programmed cell death protein-1 (PD-1), can deliver durable antitumor effects, most patients with cancer fail to respond. Recent studies suggest that ICI efficacy correlates a higher load of tumor-specific neoantigens and development vitiligo in melanoma. Here, we report low melanoma neoantigen burdens who responded had tumors expression pigmentation-related genes. Moreover, expansion peripheral blood CD8+ T populations specific for...
Tumor-associated macrophages (TAM) are regulators of extracellular matrix (ECM) remodeling and metastatic progression, the main cause cancer-associated death. We found that disabled homolog 2 mitogen-responsive phosphoprotein (DAB2) is highly expressed in tumor-infiltrating TAMs its genetic ablation significantly impairs lung metastasis formation. DAB2-expressing TAMs, mainly localized along tumor-invasive front, participate integrin recycling, ECM remodeling, directional migration a...
The majority of current image-based road following algorithms operate, at least in part, by assuming the presence structural or visual cues unique to roadway.As a result, these are poorly suited task tracking unstructured roads typical desert environments.In this paper, we propose algorithm that operates selfsupervised learning regime, allowing it adapt changing conditions while making no assumptions about general structure appearance surface.An application optical flow techniques, paired...
Summary COVID-19 has caused over 1 million deaths globally, yet the cellular mechanisms underlying severe disease remain poorly understood. By analyzing several thousand plasma proteins in 306 patients and 78 symptomatic controls serial timepoints using two complementary approaches, we uncover host immune non-immune not previously linked to this disease. Integration of proteomics with nine published scRNAseq datasets shows that SARS-CoV-2 infection upregulates monocyte/macrophage,...
Many cancer patients treated with immune checkpoint blockade (ICB) do not have durable treatment responses. Circulating biomarkers the potential to identify primary resistance or early progression on therapy alter course and avoid unnecessary toxicity. Unbiased multimodal proteomic profiling in blood has been underexplored due previously limited scalability of multiplexing technologies cohorts lacking time-series sampling. To address this, we performed plasma >2,900 proteins...