A5014721829- Monoclonal and Polyclonal Antibodies Research
- Rheumatoid Arthritis Research and Therapies
- Insect and Arachnid Ecology and Behavior
- Systemic Lupus Erythematosus Research
- Insects and Parasite Interactions
- Hepatitis B Virus Studies
- Insect and Pesticide Research
- T-cell and B-cell Immunology
- Plant and animal studies
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Hepatitis C virus research
- Hemiptera Insect Studies
- Immune Cell Function and Interaction
- Forest Insect Ecology and Management
- Animal and Plant Science Education
- Viral gastroenteritis research and epidemiology
- Chronic Lymphocytic Leukemia Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Advanced Biosensing Techniques and Applications
- Vector-borne infectious diseases
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
VA Palo Alto Health Care System
2016-2025
Stanford University
2016-2025
Geriatric Research Education and Clinical Center
2012-2025
Stanford Medicine
1976-2024
University of Pittsburgh
2024
Pfizer (United States)
2024
University of California, Los Angeles
2024
Southern Illinois University School of Medicine
2024
Palo Alto University
2010-2023
Stratford University
2016-2023
DNA polymerase activity was detected in each of eight preparations concentrated human hepatitis B antigen (HBAg) rich Dane particles prepared by high-speed centrifugation antigen-positive plasma and none seven control the same way from HBAg-negative plasma. The incorporation (3)H-thymidine-methyl-5'-triphosphate into dependent on four deoxyribonucleoside triphosphates MgCl(2). Treatment HBAg with nonionic detergent Nonidet P-40 (NP40) more than doubled enzyme activity. Fractionation...
Four patients with chronic hepatitis B infection and active were treated human leukocyte interferon. Three of them had consistently elevated levels circulating Dane-particle markers, including Dane-particle-associated DNA polymerase activity, core antigen DNA. Parenteral interferon administration at a dosage between 6.0 X 103 17 x 104 U per kilogram day was associated rapid reproducible fall in all markers the three patients. The suppressive effect transient when given for 10 days or less...
Rheumatoid arthritis (RA) is a prototypical autoimmune affecting nearly 1% of the world population and significant cause worldwide disability. Though prior studies have demonstrated appearance RA-related autoantibodies years before onset clinical RA, pattern immunologic events preceding development RA remains unclear. To characterize evolution autoantibody response in preclinical phase we used novel multiplex autoantigen array to evaluate anti-citrullinated protein antibodies (ACPA)...
Local catabolism of the amino acid tryptophan (Trp) by indoleamine 2,3-dioxygenase (IDO) is considered an important mechanism regulating T cell immunity. We show that IDO transcription was increased when myelin-specific cells were stimulated with tolerogenic altered self-peptides. Catabolites Trp suppressed proliferation and inhibited production proinflammatory helper-1 (T(H)1) cytokines. N-(3,4,-Dimethoxycinnamoyl) anthranilic (3,4-DAA), orally active synthetic derivative metabolite acid,...
COVID-19 is associated with a wide range of clinical manifestations, including autoimmune features and autoantibody production. Here we develop three protein arrays to measure IgG autoantibodies connective tissue diseases, anti-cytokine antibodies, anti-viral antibody responses in serum from 147 hospitalized patients. Autoantibodies are identified approximately 50% patients but less than 15% healthy controls. When present, largely target autoantigens rare disorders such as myositis, systemic...
A virus given the name ground squirrel hepatitis (or GSHV), with many of unique characteristics human B (HBV), has been found in Beechey squirrels northern California. Common features include morphology, viral DNA size and structure, a virion polymerase that repairs single-stranded region DNA, crossreacting antigens, persistent infection antigen continuously blood. Although similar, GSHV HBV Are not identical. The slightly greater diameter, surface antigens crossreact only partially and,...
While various monocyte chemokine systems are increased in expression osteoarthritis (OA), the hierarchy of chemokines and receptors mediating monocyte/macrophage recruitment to OA joint remains poorly defined. Here, we investigated relative contributions CCL2/CCR2 versus CCL5/CCR5 axes pathogenesis.Ccl2-, Ccr2-, Ccl5- Ccr5-deficient control mice were subjected destabilisation medial meniscus surgery induce OA. The pharmacological utility blocking signalling mouse was using bindarit, a CCL2...
To examine the degree to which shared risk factors explain relationship of periodontitis (PD) rheumatoid arthritis (RA) and determine associations PD Porphyromonas gingivalis with pathologic clinical features RA.Patients RA (n = 287) patients osteoarthritis as disease controls 330) underwent a standardized periodontal examination. The HLA-DRB1 status all participants was imputed using single-nucleotide polymorphisms from extended major histocompatibility complex. Circulating anti-P...
under greater economic stress in our society exhibit a higher rate of divorce. Additional clarification these points can be achieved by research which is pointed toward problems: (1) Specification the further sociological dimensions factors familial relations; (2) Determination relative pressures on various strata population, as felt those strata;28 and (3) Separation socio-economic from occupational marital relationships. The present suggestions are presented, then, tentative steps directions.
Abstract Introduction Osteoarthritis (OA) is a degenerative disease characterized by cartilage breakdown in the synovial joints. The presence of low-grade inflammation OA joints receiving increasing attention, with synovitis shown to be present even early stages disease. How arises unclear, but proteins fluid affected could conceivably contribute. We therefore surveyed and assessed their immunostimulatory properties. Methods used mass spectrometry survey patients knee OA. multiplex...
Abstract Objective Rheumatoid arthritis (RA) is associated with the presence of anti–citrullinated protein antibodies (ACPAs). Nearly two‐thirds patients ACPA‐positive RA have immune complexes that contain citrullinated fibrinogen, and these fibrinogen–containing (cFb‐IC) can exacerbate disease in murine models RA; however, exact role such ACPA ICs pathogenesis has remained elusive. We undertook present study to investigate a novel mechanism by which ACPAs specifically targeting fibrinogen...
Rheumatoid arthritis is associated with the development of autoantibodies to citrullinated self-proteins. Citrullinated synovial proteins, which are generated via actions protein arginine deiminases (PADs), known develop in murine collagen-induced (CIA) model inflammatory arthritis. Given these findings, we evaluated whether N-α-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide (Cl-amidine), a recently described pan-PAD inhibitor, could affect and autoimmunity by treating mice CIA...
Particles containing DNA polymerase (Dane particles) were purified from the plasma of chronic carriers hepatitis B antigen. After a reaction with Dane particle preparations treated Nonidet P-40 detergent, core structures radioactive product isolated by sedimentation in sucrose density gradient. The was extracted sodium dodecyl sulfate and band preformed CsCl Examination electron microscopy revealed exclusively circular double-stranded molecules approximately 0.78 mum length. Identical...
Neutrophil extracellular traps containing citrullinated peptides promote inflammation in synovial fibroblasts.