A5067533777- Pancreatic and Hepatic Oncology Research
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Ubiquitin and proteasome pathways
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Heme Oxygenase-1 and Carbon Monoxide
- Food Quality and Safety Studies
- Porphyrin Metabolism and Disorders
- Cancer, Lipids, and Metabolism
- Nutrition, Health and Food Behavior
- Virology and Viral Diseases
- Virus-based gene therapy research
- Sphingolipid Metabolism and Signaling
- Neonatal Health and Biochemistry
- Food Safety and Hygiene
- Peptidase Inhibition and Analysis
- Iron Metabolism and Disorders
- Peroxisome Proliferator-Activated Receptors
- Protease and Inhibitor Mechanisms
- Extracellular vesicles in disease
- RNA Research and Splicing
- Tannin, Tannase and Anticancer Activities
University of Illinois Chicago
2023
David H. Murdock Research Institute
2019-2020
University of North Carolina at Chapel Hill
2020
Carolinas Healthcare System
2010-2018
George Washington University
1992-2018
Carolinas Medical Center
2011-2014
Seoul National University
2013
University of North Carolina at Charlotte
2009-2011
Mayo Clinic in Arizona
2011
University of Connecticut
2004-2009
Protein phosphorylation events during T cell receptor (TCR) signaling control the formation of complexes among proteins proximal to TCR, activation kinase cascades, and transcription factors; however, mode extent influence in coordinating diverse phenomena associated with are unclear. Therefore, we used human Jurkat leukemia line as a model system performed large-scale quantitative phosphoproteomic analyses TCR signaling. We identified 10,665 unique sites, which 696 showed TCR-responsive...
GPCR inhibitors are highly prevalent in modern therapeutics. However, interference with complex regulatory mechanisms leads to both therapeutic efficacy and adverse effects. Recently, the sphingosine-1-phosphate (S1P) receptor inhibitor FTY720 (also known as Fingolimod), which induces lymphopenia prevents neuroinflammation, was adopted a disease-modifying multiple sclerosis. Although efficacious, dose-dependent increases events have tempered its utility. We show here that FTY720P...
Cardiovascular disease presents significant variations in human populations with respect to the atherosclerotic plaque progression, inflammation, thrombosis, and rupture. To gain a more comprehensive picture of pathogenic mechanism atherosclerosis seen patients, efficient methods identify proteins from normal diseased arteries need be developed. accomplish this goal, we tested feasibility efficiency protein identification by recently developed method, termed direct tissue proteomics (DTP)....
MUC1 is overexpressed and aberrantly glycosylated in more than 60% of pancreatic ductal adenocarcinomas. The functional role cancer has yet to be fully elucidated due a dearth appropriate models. In this study, we have generated mouse models that spontaneously develop adenocarcinoma (KC), which are either Muc1-null (KCKO) or express human (KCM). We show KCKO mice significantly slower tumor progression rates secondary metastasis, compared with both KC KCM. Cell lines derived from tumors less...
Abstract A proteomic map for human urine on two‐dimensional (2‐D) gels has been developed. Initial studies demonstrated that the proteins prepared by conventional methods showed interference and poor reproducibility in 2‐D electrophoresis (2‐DE). To address this issue, samples were dialyzed to remove any interfering molecules. The dialysis of concentration lyophilization without fractionation significantly improved resolution likely represents total a gel. In addition, removing albumin from...
Myelin, formed by oligodendrocytes (OLs) in the CNS, is critical for axonal functions, and its damage leads to debilitating neurological disorders such as multiple sclerosis. Understanding molecular mechanisms of myelination pathogenesis human myelin disease has been limited partly relative lack identification functional characterization repertoire proteins. Here, we present a large-scale analysis proteome, using shotgun approach 1-dimensional PAGE liquid chromatography/tandem MS. Three...
Werner syndrome protein (WRN) is a multifunctional enzyme with helicase, ATPase, and exonuclease activities that are necessary for numerous DNA-related transactions in the human cell. Recent studies identified WRN as synthetic lethal target cancers characterized by genomic microsatellite instability resulting from defects DNA mismatch repair pathways. WRN's helicase activity essential viability of these high (MSI-H) thus presents therapeutic opportunity. To this end, we developed multiplexed...
Global understanding of tissue-specific differences in mitochondrial signal transduction requires comprehensive protein identification from multiple cell and tissue types. Here, we explore the feasibility efficiency using one-dimensional gel electrophoresis combination with nano liquid-chromatography tandem mass spectrometry (GeLC-MS/MS). The use only 40 mug purified proteins data analysis stringent scoring criteria molecular validation slices enables 227 known (membrane soluble) 453...
Proteomic analysis of extracellular vesicles (EVs) from biological fluid is a powerful approach to discover potential biomarkers for human diseases including cancers, as EV secreted fluids are originated the affected tissue. In order investigate significant molecules related pathogenesis bladder cancer, EVs were isolated patient urine which was analyzed by mass spectrometry based proteomics. Comparison proteome whole demonstrated an increased number protein identification in EV. Comparative...
Identification and characterization of the nuclear proteome is important for detailed understanding multiple signaling events in eukaryotic cells. Toward this goal, we extensively characterized human T leukemia cells by sequential extraction proteins with different physicochemical properties using three buffer conditions. This large scale proteomic study also tested feasibility technical challenges associated stable isotope labeling amino acids cell culture (SILAC) to uncover quantitative...
Abstract Background Vesicular stomatitis virus (VSV) is the prototypic rhabdovirus and best studied member of order Mononegavirales . There now compelling evidence that enveloped virions released from infected cells carry numerous host (cellular) proteins some which may play an important role in viral replication. Although several cellular have been previously shown to be incorporated into VSV virions, no systematic study has done reveal protein composition for or any other Results Here we...
Hepatocellular carcinoma (HCC) is one of the leading causes mortality from solid organ malignancy worldwide. Because complexity proteins within liver cells and tissues, discovery therapeutic targets HCC has been difficult. To investigate strategies for decreasing tissue samples detecting meaningful protein mediators HCC, we employed subcellular fractionation combined with 1D-gel electrophoresis liquid chromatography-tandem mass spectrometry analysis. Moreover, utilized a statistical method,...
A global protein survey is needed to gain systems-level insights into mammalian cell signaling and information flow. Human Jurkat T leukemic cells are one of the most important model systems for study, but no comprehensive proteomics has been carried out in this type. In present study we combined subcellular fractionation, multiple enrichment methods, replicate tandem mass spectrometry analyses determine expression pattern a single The proteome dataset was evaluated by comparison with...
Summary. Chronic hepatitis C (CHC) infection caused by virus (HCV) is a major cause of liver disease and remains therapeutic challenge. A variety host proteins interact with HCV proteins. The definitive role cytoskeletal (CS) in to be determined. In this study, our aim was determine the expression profile differentially regulated expressed selected CS their association infected hepatocytes as possible targets. Using proteomics, qRT-PCR, Western blot immunofluorescence techniques, we revealed...
Virus particles (virions) often contain not only virus-encoded but also host-encoded proteins. Some of these host proteins are enclosed within the virion structure, while others, in case enveloped viruses, embedded host-derived membrane. While many protein incorporations likely accidental, some may play a role virus infectivity, replication and/or immunoreactivity next host. Host be especially important therapeutic applications where large numbers administered. Vesicular stomatitis (VSV) is...
Erythropoietic protoporphyria (EPP) and X-linked (XLP) are genetic abnormalities of heme synthesis that result in excess production protoporphyrin manifest as severe photosensitivity. These disorders often associated with iron deficiency anaemia (IDA). Our aim was to determine whether hepcidin is increased EPP/XLP patients, resulting decreased enteral absorption IDA.Eight subjects EPP, one XLP nine controls had baseline blood urine samples collected, thereafter were given oral ferrous...