A5014220234- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Prostate Cancer Treatment and Research
- Immune Response and Inflammation
- Cancer, Lipids, and Metabolism
- Immune cells in cancer
- Inflammatory Biomarkers in Disease Prognosis
- Asthma and respiratory diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Platelet Disorders and Treatments
- Physiological and biochemical adaptations
- Click Chemistry and Applications
- Mitochondrial Function and Pathology
- Peroxisome Proliferator-Activated Receptors
- Inflammatory mediators and NSAID effects
- Metabolism and Genetic Disorders
- MicroRNA in disease regulation
- Synthesis and Biological Evaluation
- Synthesis and biological activity
- Insect and Pesticide Research
- Immune Cell Function and Interaction
- Cancer, Stress, Anesthesia, and Immune Response
- Hydrogen's biological and therapeutic effects
- Herbal Medicine Research Studies
- Sperm and Testicular Function
Université de Moncton
2015-2024
Centre hospitalier universitaire de Québec
2015-2019
Université Laval
2009-2019
Institut Universitaire de Cardiologie et de Pneumologie de Québec
2010-2018
Fraternal Order of Eagles
2017
University of Utah
2017
University of Iowa
2017
Significance On activation, blood platelets package components from their cytoplasm into microparticles (MPs), tiny vesicles released by cytoplasmic membrane budding and shedding. Given that MPs can impact other cellular lineages on internalization, we aimed to decipher the mechanisms promoting MP internalization recipients. We modeled neutrophils identified a predominant lipid, 12(S)-hydroxyeicosatetranoic acid, as mediator critical for promotion of internalization. were found inside...
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days storage. A combination flow cytometry spanning-tree progression analysis density-normalized events (SPADE) was used determine MP during All PC biochemical...
Mitochondria have been suggested to be paramount for temperature adaptation in insects. Considering the large range of environments colonized by this taxon, we hypothesized that species surviving changes would those with most flexible mitochondria. We thus investigated responses mitochondrial oxidative phosphorylation (OXPHOS) three flying insects: honeybee (Apis mellifera carnica), fruit fly (Drosophila melanogaster) and Colorado potato beetle (Leptinotarsa decemlineata). Specifically,...
Background 5-lipoxygenase (5-LO) catalyses the transformation of arachidonic acid (AA) into leukotrienes (LTs), which are important lipid mediators inflammation. LTs have been directly implicated in inflammatory diseases like asthma, atherosclerosis and rheumatoid arthritis; therefore inhibition LT biosynthesis is a strategy for treatment these chronic diseases. Methodology/Principal Findings Analogues caffeic acid, including naturally-occurring phenethyl ester (CAPE), were synthesized...
Background It is well established that inflammation and platelets promote multiple processes of cancer malignancy. Recently, have received attention for their role in carcinogenesis through the production microvesicles or platelet-derived microparticles (PMPs), which transfer biological content to cells. We previously characterized a new subpopulation these (termed mito-microparticles), package functional mitochondria. The potential mitochondria cells particularly impactful as many aspects...
Triple-negative breast cancer (TNBC) cells' secretome can induce a pro-inflammatory phenotype in human adipose-derived mesenchymal stem cells (hADMSC). This be prevented by the green tea polyphenol epigallocatechin-3-gallate (EGCG). The impact of EGCG on paracrine regulation that extracellular vesicles (EVs) specifically exert within TNBC remains unknown.EVs were obtained from TNBC-derived serum-starved MDA-MB-231 cell model treated or not with under normoxic hypoxic (< 1% O2) culture...
Alkyne and azide analogs of natural compounds that can be coupled to sensitive tags by click chemistry are powerful tools study biological processes. Arachidonic acid (AA) is a FA precursor biologically active compounds. 19-Alkyne-AA (AA-alk) clickable AA analog; however, its use as surrogate metabolism requires further evaluation. In this study, AA-alk was compared with in human cells. Jurkat cell uptake 2-fold greater than AA-alk, but significantly more AA-Alk elongated 22:4. incorporation...
Abstract An increasing number of studies show that platelets as well platelet-derived microparticles (PMP) play significant roles in cancer malignancy and disease progression. Particularly, PMPs have the capacity to interact internalize within target cells resulting transfer their bioactive cargo, which can modulate signaling activation processes recipient cells. We recently identified a new subpopulation these vesicles (termed mitoMPs), contain functional mitochondria. Given predominant...
On activation, platelets increase glucose uptake, glycolysis, and oxidation consume stored glycogen. This correlation between metabolism platelet function is not well understood even less known about the role of on in vivo. For to enter a cell, it must be transported through transporters. Here we evaluate contribution GLUT3 (glucose transporter 3) better understand platelets.Platelet-specific knockout was generated by crossing mice harboring floxed allele PF4 (platelet factor 4)-driven Cre...
The fruit fly, Drosophila melanogaster, represents an emerging model for the study of metabolism. Indeed, drosophila have structures homologous to human organs, possess highly conserved metabolic pathways and a relatively short lifespan that allows different fundamental mechanisms in period time. It is, however, surprising one essential cellular metabolism, mitochondrial respiration, has not been thoroughly investigated this model. is likely because measure respiration usually requires very...
Abstract Leukotriene B4 (LTB4) plays a prominent role in innate immunity as it induces phagocyte recruitment, the release of antimicrobial effectors, and potentiates ingestion killing pathogens. In humans, LTB4 has short half-life is rapidly metabolized by leukocytes, notably into 20-OH- 20-COOH-LTB4 neutrophils. Although these metabolites bind to BLT1 receptor with high affinity, they activate neutrophils much lower extent than LTB4. We thus postulated that could dampen BLT1-mediated...
Soxhlet (SE), microwave-assisted (MAE) and ultrasound-assisted (UAE) extraction were compared using ten solvents for their efficiency to extract phenolic flavonoid antioxidants from Eastern Canada propolis. Extracts total (TPC) (TFC) content, radical scavenging activities. Anti-inflammatory activity through inhibition of 5-lipoxygenase (5-LO) products biosynthesis in HEK293 cells was also evaluated. The results showed that SE extracts polar had the highest TPC TFC. obtained with ethanol,...
5-Lipoxygenase (5-LO) is the essential enzyme for biosynthesis of leukotrienes, important mediators inflammation. This study investigated whether variants 5-LO exist in human leukocytes. mRNA isoforms that are consistent with alternative splicing were identified by RT-PCR a cell line or type-specific pattern. All evaluated cells expressed containing all 14 exons expected sites. Individual retained intron 10 (α-10), lacked exon 13 (Δ-13), and (Δ-10,13) first 96 base pairs (Δ-p10) identified....
Summary Increased intracellular reactive oxygen species (ROS) promote platelet activation. The sources of platelet-derived ROS are diverse and whether or not mitochondrial derived ROS, modulates function is incompletely understood. Studies platelets from patients with sickle cell disease, diabetes suggest a correlation between dysfunction. Therefore, we generated mice specific knockout superoxide dismutase 2 (SOD2-KO) to determine if increased increases SOD2-KO demonstrated decreased SOD2...
5-Lipoxygenase (5-LO) catalyzes leukotriene (LT) biosynthesis by a mechanism that involves interactions with 5-lipoxygenase activating protein (FLAP) and coactosin-like (CLP). 5-LO splice variants were recently identified in human myeloid lymphoid cells, including the catalytically inactive ∆13 isoform (5-LO∆13) whose transcript lacks exon 13. 5-LO∆13 inhibits product when co-expressed active full length (5-LO1). The objective of this study was to investigate potential mechanisms which...
Leukotrienes are inflammatory mediators that actively participate in the response and host defense against pathogens. However, leukotrienes also chronic diseases. 5-lipoxygenase is a key enzyme biosynthesis of thus validated therapeutic target. As today, zileuton remains only clinically approved inhibitor; however, its use has been limited due to severe side effects some patients. Hence, search for better inhibitor continues. In this study, we investigated structural analogues caffeic acid...
The isolation of mitochondria is gaining importance in experimental and clinical laboratory settings. mitochondrion known as the powerhouse cell it produces energy to power most cellular functions but also involved many processes. Of interest, mitochondrial components (i.e. circular DNA, N-formylated peptides, cardiolipin) have been several human inflammatory pathologies, such cancer, Alzheimer's disease, Parkinson's rheumatoid arthritis. Therefore, stringent methods purification are utmost...