A5058621938- Urinary Tract Infections Management
- Extracellular vesicles in disease
- Platelet Disorders and Treatments
- Bladder and Urothelial Cancer Treatments
- Pelvic floor disorders treatments
- Diabetes and associated disorders
- Phagocytosis and Immune Regulation
- Urinary Bladder and Prostate Research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Gut microbiota and health
- Erythrocyte Function and Pathophysiology
- Dermatology and Skin Diseases
- Escherichia coli research studies
- Complement system in diseases
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Myasthenia Gravis and Thymoma
- Growth Hormone and Insulin-like Growth Factors
- Influenza Virus Research Studies
- Lysosomal Storage Disorders Research
- Learning Styles and Cognitive Differences
Institut Pasteur
2016-2023
Inserm
2011-2023
Institut Cochin
2023
Université Paris Cité
2023
Centre National de la Recherche Scientifique
2023
Centre hospitalier universitaire de Québec
2014-2019
Université Laval
2013-2019
Canadian Natural Resources
2015
Nantes Université
2011
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2011
Significance On activation, blood platelets package components from their cytoplasm into microparticles (MPs), tiny vesicles released by cytoplasmic membrane budding and shedding. Given that MPs can impact other cellular lineages on internalization, we aimed to decipher the mechanisms promoting MP internalization recipients. We modeled neutrophils identified a predominant lipid, 12(S)-hydroxyeicosatetranoic acid, as mediator critical for promotion of internalization. were found inside...
Exosome-like vesicles containing an active 20 S proteasome core contribute to autoimmunity and vascular allograft inflammation.
Platelet microparticles (MPs) represent the most abundant MPs subtype in circulation, and can mediate intercellular communication through delivery of bioactives molecules, such as cytokines, proteins, lipids RNAs. Here, we show that platelet be internalised by primary human macrophages deliver functional miR-126-3p. The increase macrophage miR-126-3p levels was not prevented actinomycin D, suggesting it due to de novo gene transcription. dose-dependently downregulated expression four...
Sex-based differences influence incidence and outcome of infectious disease. Women have a significantly greater urinary tract infection (UTI) than men, yet, conversely, male UTI is more persistent with associated morbidity. Mechanisms underlying these sex-based are unknown, in part due to lack experimental models. We optimized model transurethrally infect mice directly compared both sexes. Although sexes were initially equally colonized by uropathogenic E. coli, only testosterone-treated...
Microparticles, also called microvesicles, are submicron extracellular vesicles produced by plasma membrane budding and shedding recognized as key actors in numerous physio(patho)logical processes. Since they can be released virtually any cell lineages retrieved biological fluids, microparticles appear potent biomarkers. However, the small dimensions of soluble factors present body fluids considerably impede their quantification. Here, flow cytometry with improved methodology for...
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days storage. A combination flow cytometry spanning-tree progression analysis density-normalized events (SPADE) was used determine MP during All PC biochemical...
Urinary tract infection (UTI) is one of the most prevalent human bacterial infections. New therapeutic approaches, including vaccination and immunotherapy, are urgently needed to combat rapid global dissemination multidrug-resistant uropathogens. Development therapies impeded by an incomplete understanding memory development during UTI. Here, we found that reducing load early in infection, inoculum or with antibiotics after completely abrogated protective response. We observed a mixed T...
Resident macrophages are abundant in the bladder, playing key roles immunity to uropathogens. Yet, whether they heterogeneous, where come from, and how respond infection remain largely unknown. We identified two macrophage subsets mouse bladders, MacM muscle MacL lamina propria, each with distinct protein expression transcriptomes. Using a urinary tract model, we validated our transcriptomic analyses, finding that phagocytosed more bacteria polarized an anti-inflammatory profile, whereas...
Urinary tract infection (UTI) is a very common infection. Approximately 25% of all women will experience recurrent infection, defined by 2 UTI in 6 months or 3 12 month period. Recurrent thought to be due part the development local bacterial reservoirs bladder after an These are undetected host immune system and may re-emerge resolution primary Frequently, require repeated antibiotic use, leading resistance, negatively impacting quality life patient. Given rise multidrug resistant...
Catheter-associated urinary tract infections (CAUTI) are the most common hospital-associated infections. Here, we report that bladder catheterization initiated a persistent sterile inflammatory reaction within minutes of catheter implantation. Catheterization resulted in increased expression genes associated with defense responses and cellular migration, ensuing rapid sustained innate immune cell infiltration into bladder. also hypersensitivity to Enterococcus faecalis uropathogenic...
Human herpesvirus 6B (HHV-6B) is a ubiquitous pathogen with frequent reactivation observed in immunocompromised patients such as BM transplant (BMT) recipients. Adoptive immunotherapy promising therapeutic avenue for the treatment of opportunistic infections, including herpesviruses. While T-cell can successfully control CMV and EBV reactivations BMT recipients, therapy not available HHV-6 part due to lack identified protective CD8(+) epitopes. Our goal was identify viral epitopes derived...
Abstract Sex-based differences influence incidence and outcome of infectious disease. Women have a significantly greater urinary tract infection (UTI) than men, yet, conversely, male UTI is more persistent with associated morbidity. Mechanisms underlying these sex-based are unknown, in part due to lack experimental models. We optimized model transurethrally infect mice directly compared both sexes. Although sexes were initially equally colonized by uropathogenic E. coli , only...
BACKGROUND: Bladder cancer is the fourth most common for men. However, women are often diagnosed with later stage disease and have poorer outcomes. Whether immune-based sex differences contribute to this discrepancy unclear. In addition, models investigate tumor-specific immunity in bladder cancer, context of tumor development or response therapy, lacking. OBJECTIVE: To address specific unmet need, we incorporated a commonly used model antigen, ovalbumin, into two well-established cancer;...
The thymus is a primary lymphoid organ, home of maturation and selection thymocytes for generation functional T-cells. Multiple factors are involved throughout the different stages process to tightly regulate T-cell production. metabolism arachidonic acid by cyclooxygenases, lipoxygenases specific isomerases generates eicosanoids, lipid mediators capable triggering cellular responses. In this study, we determined profile expression eicosanoids present in mouse at thymocyte development. As...
Abstract Urinary tract infection (UTI) is one of the most prevalent human bacterial infections. New therapeutic approaches, including vaccination and immunotherapy, are urgently needed to combat rapid global dissemination multidrug-resistant uropathogens. Development therapies impeded by an incomplete understanding memory development during UTI. Here, we found that reducing load early in infection, inoculum or with antibiotics after completely abrogated protective response. We observed a...
Bacterial prostatitis affects 1% of men, with increased incidence in the elderly. Acute bacterial frequently progresses to chronicity, marked by recurrent episodes interspersed asymptomatic periods variable duration. Antibiotic treatment is standard care; however, dissemination antimicrobially resistant uropathogens threatens therapy efficacy. Thus, development nonantibiotic-based approaches treat chronic disease a priority. Currently, why arises unclear, as immune response prostate...
Summary Resident macrophages are abundant in the bladder, playing key roles immunity to uropathogens. Yet, whether they heterogeneous, where come from, and how respond infection remain largely unknown. We identified two macrophage subsets mouse bladders, MacM muscle MacL lamina propria, with distinct protein expression transcriptomes. Using a urinary tract model, we validated our transcriptomic analyses, finding that phagocytosed more bacteria polarized anti-inflammatory profile, whereas...
Abstract Bladder cancer is the fourth most common for men. However, women are often diagnosed with later stage disease and have poorer outcomes. Whether immune-based sex differences contribute to this discrepancy unclear. In addition, models investigate tumor-specific immunity in bladder cancer, context of tumor development or response therapy, lacking. To address specific unmet need, we incorporated a commonly used model antigen, ovalbumin, into two well-established cancer; orthotopic MB49...