A5075297424- Sphingolipid Metabolism and Signaling
- Effects of Radiation Exposure
- Photodynamic Therapy Research Studies
- Free Radicals and Antioxidants
- Angiogenesis and VEGF in Cancer
- Neonatal Respiratory Health Research
- Neuroscience of respiration and sleep
- Lipid metabolism and disorders
- Hematopoietic Stem Cell Transplantation
- Platelet Disorders and Treatments
- Obstructive Sleep Apnea Research
- Endoplasmic Reticulum Stress and Disease
- Chemotherapy-induced cardiotoxicity and mitigation
- Tracheal and airway disorders
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Blood groups and transfusion
- Infant Health and Development
- Airway Management and Intubation Techniques
- Venous Thromboembolism Diagnosis and Management
- Blood properties and coagulation
- Galectins and Cancer Biology
- Eicosanoids and Hypertension Pharmacology
- Glutathione Transferases and Polymorphisms
- Radiopharmaceutical Chemistry and Applications
Inserm
2007-2020
Nantes Université
2007-2020
Centre National de la Recherche Scientifique
2012-2020
Institut de Radioprotection et de Sûreté Nucléaire
2004-2017
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2007-2017
Bipar
2013
Hopitaux Civils de Colmar
2007-2012
Memorial Sloan Kettering Cancer Center
2010
Hôpital d'Hautepierre
2001-2007
Hôpitaux Universitaires de Strasbourg
2001-2006
Adhesion molecules play a key role in cellular traffic through vascular endothelium, particular during the inflammatory response when leukocytes migrate from blood into tissues. Since inflammation is one of major consequences radiation injury, we investigated effect ionizing on cell-surface expression intercellular adhesion molecule-1 (ICAM-1), cell (VCAM-1) and E-selectin cultured human umbilical vein endothelial cells (HUVEC). Flow cytometry performed irradiated HUVEC revealed both time-...
Human erythroblastic precursor cells adhere to fibronectin (Fn) but the exact nature of receptors mediating this interaction has not been characterized. In study, we report data showing that immature human erythroblasts express integrins VLA-4 and VLA-5 both these molecules act as on cells. We have recently demonstrated adhesion Fn purified CFU-E their immediate progeny preproerythroblasts was inhibited by antibodies directed against receptor (VLA-5). Here extended those results...
Gaugler, M. H., Vereycken-Holler, V., Squiban, C., Vandamme, M., Vozenin-Brotons, C. and Benderitter, Pravastatin Limits Endothelial Activation after Irradiation Decreases the Resulting Inflammatory Thrombotic Responses. Radiat. Res. 163, 479–487 (2005).Endothelial dysfunction has been implicated in pathogenesis of atherosclerosis, fibrosis vascular occlusion radiation therapy. Statins have reported to improve endothelial function; however, this beneficial effect on cells never investigated...
Objective. Methylxanthines and doxapram are currently used to treat apnea of prematurity but not fully effective often present undesirable side effects. The study examines whether exposure an odor known modulate the infant's respiratory rate could reduce frequency apneic spells. Method. Fourteen preterm newborns born at 24 28 gestational weeks presenting recurrent despite caffeine therapy were exposed a pleasant diffused during hours in incubator. Efficiency olfactory treatment was judged by...
A previous in vitro study showed that sphingosine-1-phosphate (S1P), a ceramide antagonist, preserved endothelial cells culture from radiation-induced apoptosis. We proposed to validate the role of S1P tissue radioprotection by inhibiting acute gastrointestinal (GI) syndrome induced cell apoptosis after high dose radiation. Retro-orbital was injected mice exposed 15 Gy, dose-inducing GI within 10 days. Overall survival and on intestines sections were studied. Intestinal type targeted early...
Abstract Because of the central role endothelium in tissue homeostasis, protecting vasculature from radiation-induced death is a major concern radioprotection. Premitotic apoptosis and mitotic are two prevalent cell pathways induced by ionizing radiation. Endothelial cells undergo after radiation through generation sphingolipid ceramide. However, if known as established pathway for cycling eukaryotic cells, direct involvement proliferating endothelial radiosensitivity has not been clearly...
Irradiation exposure is known to induce an inflammatory reaction. Endothelial cells play a crucial role both in the process and radiation damage. Therefore, supernatants cell lysates of (60)Co-irradiated human umbilical vein endothelial (HUVEC) have been assessed for presence pro-inflammatory cytokines. After gamma irradiation, interleukin (IL)-1alpha, IL-1beta tumor necrosis factor (TNF)-alpha remained undetectable lysates. However, dose-dependent increase production IL-6 IL-8 has...
Endothelial cells (ECs) are a critical component of the bone marrow stroma in regulation haemopoiesis. Recovery aplasia after radiation exposure depends, part, on repair radiation‐induced endothelial damage. Therefore, we assessed ability an irradiated human EC line (TrHBMEC) to support transmigration, proliferation and differentiation CD34 + either or not transendothelial migration co‐culture models. Radiation‐induced damage was reflected by increased release soluble intercellular adhesion...
Van der Meeren, A., Vandamme, M., Squiban, C., Gaugler, M-H. and Mouthon, M-A. Inflammatory Reaction Changes in Expression of Coagulation Proteins on Lung Endothelial Cells after Total-Body Irradiation Mice. Radiat. Res. 160, 637–646 (2003).Inflammatory reaction is a classical feature radiation exposure, pneumonitis dose-limiting complication the handling hematological disorders treated with total-body irradiation. In present study, we first evaluated inflammatory response C57BL6/J mice...
The response of endothelial cells (EC) to high radiation doses leads damage normal tissue or tumor. precise mechanisms the endothelial-tissue linkage are still largely unknown. We investigated possible involvement a bystander effect, secondary damage, in radiation. Proliferating human intestinal epithelial T84 were grown non-contact co-culture with confluent primary microvascular EC (HMVEC-L). unirradiated co-cultured irradiated was studied by evaluating cell growth, death and morphology....
Endothelial cell dysfunction is a classic consequence of radiation damage. Bone marrow endothelial cells (BMEC) are critical component the stroma in regulation haemopoiesis. In animal models, radiation‐induced injury BMEC has been described and role for haemopoietic regeneration after irradiation suggested. However, functions involved have not assessed. Therefore we studied functional response human to using transformed line (TrHBMEC) irradiated with 2, 5 or 10 Gy. Our results showed time‐...
Purpose: Ovarian peritoneal carcinomatosis is a pathology for which effective cures are currently lacking. New research protocols seek to eradicate residual micrometastases following cytoreductive surgery by using Hyperthermic Intraperitoneal Chemotherapy (HIPEC), or Radioimmunotherapy (RIT). This study aims firstly develop alpha-RIT an anti-CD138 mAb radiolabeled with alpha-emitter, bismuth 213 (213Bi-B-B4) and HIPEC in nude mouse model, secondly compare combine these techniques. Material...
Bertho, J-M., Prat, M., Frick, J., Demarquay, C., Gaugler, M-H., Dudoignon, N., Clairand, I., Chapel, A., Gorin, N-C., Thierry, D. and Gourmelon, P. Application of Autologous Hematopoietic Cell Therapy to a Nonhuman Primate Model Heterogeneous High-Dose Irradiation. Radiat. Res. 163, 557– 570 (2005).We developed model heterogeneous irradiation in nonhuman primate test the feasibility autologous hematopoietic cell therapy for treatment radiation accident victims. Animals were irradiated...
Inhibiting the growth of tumor vasculature represents one relevant strategies against progression. Between all different pro-angiogenic molecular targets, plasma membrane glycosphingolipids have been under-investigated. In this present study, we explore anti-angiogenic therapeutic advantage a immunotherapy targeting globotriaosylceramide Gb3. purpose, monoclonal antibody Gb3, named 3E2 was developed and characterized. We first demonstrate that Gb3 is over-expressed in proliferative...
Ceramide-induced endothelial cell apoptosis boosts intestinal stem radiosensitivity. However, the molecular connection between these two cellular compartments has not been clearly elucidated. Here we report that ceramide and its related enzyme acid sphingomyelinase (ASM) are secreted by irradiated cells act as bystander factors to enhance radiotoxicity of epithelium. Ceramide isoforms ASM were acutely in blood serum wild-type mice after 15 Gy radiation dose, inducing a gastrointestinal...