A5047448446- Cancer Cells and Metastasis
- Digestive system and related health
- Sphingolipid Metabolism and Signaling
- 3D Printing in Biomedical Research
- Free Radicals and Antioxidants
- Photodynamic Therapy Research Studies
- Tissue Engineering and Regenerative Medicine
- Helicobacter pylori-related gastroenterology studies
- Pluripotent Stem Cells Research
- Renal and related cancers
- Gastrointestinal motility and disorders
- Congenital gastrointestinal and neural anomalies
- Clinical Nutrition and Gastroenterology
- Gut microbiota and health
- Cancer Research and Treatments
- Barrier Structure and Function Studies
- Gastric Cancer Management and Outcomes
- Biomedical Ethics and Regulation
- Diet and metabolism studies
- Genetic factors in colorectal cancer
- Microbial Metabolic Engineering and Bioproduction
- Microbial Inactivation Methods
- Eosinophilic Esophagitis
- Cancer therapeutics and mechanisms
- Food Allergy and Anaphylaxis Research
Cincinnati Children's Hospital Medical Center
2016-2025
The Enteric Nervous System in Gut and Brain Disorders
2012-2025
Inserm
2010-2025
Nantes Université
2009-2025
University of Cincinnati
2017-2023
Centre National de la Recherche Scientifique
2023
Centre Hospitalier Universitaire de Nantes
2012-2022
PhysioPathologie des Adaptations Nutritionnelles
2010
Hôpital Louis-Mourier
1987
Abstract The intestinal epithelium constitutes a system of constant and rapid renewal triggered by proliferation stem cells (ISCs), is an ideal for studying cell proliferation, migration, differentiation. Primary cultures have proven to be promising unraveling the mechanisms involved in homeostasis. In 2009, Sato et al. established long‐term primary culture generate epithelial organoids (enteroids) with crypt‐ villus‐like domains representing complete census progenitors differentiated cells....
Human intestinal organoids (HIOs) are a tissue culture model in which small intestine-like is generated from pluripotent stem cells.By carrying out unsupervised hierarchical clustering of RNA-sequencing data, we demonstrate that HIOs most closely resemble human fetal intestine.We observed genes involved digestive tract development enriched both intestine and compared to adult tissue, whereas related function Paneth cell host defense expressed at higher levels intestine.Our study also...
Although intestinal homeostasis is maintained by stem cells (ISCs), regeneration impaired upon aging. Here, we first uncover changes in architecture, cell number, and composition Second, identify a decline the regenerative capacity of ISCs aging because canonical Wnt signaling ISCs. Changes expression Wnts are found themselves their niche, including Paneth mesenchyme. Third, reactivating enhances function both murine human and, thus, ameliorates aging-associated phenotypes an organoid assay....
Human intestinal organoids (HIOs) derived from pluripotent stem cells provide a valuable model for investigating human organogenesis and physiology, but they lack the immune components required to fully recapitulate complexity of biology diseases. To address this issue begin decipher intestinal-immune crosstalk during development, we generated HIOs containing by transplanting under kidney capsule mice with humanized system. We found that temporally migrate mucosa form cellular aggregates...
Human intestinal organoids (HIOs) derived from human pluripotent stem cells co-differentiate both epithelial and mesenchymal lineages in vitro but lack important cell types such as neurons, endothelial cells, smooth muscle, which limits translational potential. Here, we demonstrate that the niche factor, EPIREGULIN (EREG), enhances HIO differentiation with epithelium, mesenchyme, enteric neuroglial populations, organized muscle a single differentiation, without need for co-culture. When...
The cytotoxin-associated gene (Cag) pathogenicity island is a strain-specific constituent of Helicobacter pylori (H. pylori) that augments cancer risk. CagA translocates into the cytoplasm where it stimulates cell signaling through interaction with tyrosine kinase c-Met receptor, leading cellular proliferation. Identified as potential gastric stem marker, cluster-of-differentiation (CD) CD44 also acts co-receptor for c-Met, but whether plays functional role in H. pylori-induced epithelial...
Wound healing of the gastrointestinal mucosa is essential for maintenance gut homeostasis and integrity. Enteric glial cells play a major role in regulating intestinal barrier function, but their mucosal repair remains unknown. The impact conditional ablation enteric glia on dextran sodium sulfate (DSS)-induced damage diclofenac-induced ulcerations was evaluated vivo GFAP-HSVtk transgenic mice. A mechanically induced model wound developed to study glial-induced epithelial restitution....
The epithelium of the gastrointestinal tract is constantly renewed as it turns over. This process triggered by proliferation intestinal stem cells (ISCs) and progeny that progressively migrate differentiate toward tip villi. These processes, essential for homeostasis, have been extensively studied using multiple approaches. Ex vivo technologies, especially primary cell cultures proven to be promising understanding epithelial functions. A long-term culture system mouse crypts has established...
Background & AimsOur goal was to develop an initial study for the proof of concept whereby gastric cancer organoids are used as approach predict tumor response in individual patients.MethodsOrganoids were derived from resected tumors (huTGOs) or normal stomach tissue collected sleeve gastrectomies (huFGOs). Organoid cultures treated with standard-of-care chemotherapeutic drugs corresponding patient treatment: epirubicin, oxaliplatin, and 5-fluorouracil. treatment correlated each whom huTGOs...
The enteric nervous system (ENS) and its major component, glial cells (EGCs), have recently been identified as a regulator of intestinal epithelial barrier functions. Indeed, EGCs inhibit cell (IEC) proliferation increase resistance IEC adhesion via the release EGC-derived soluble factors. Interestingly, EGC regulation functions is reminiscent previously reported peroxisome proliferator-activated receptor gamma (PPARgamma)-dependent functional effects. In this context, present study aimed at...
<h3>Background</h3> Enteric glial cells (EGCs) are important regulators of intestinal epithelial barrier (IEB) functions. EGC-derived <i>S</i>-nitrosoglutathione (GSNO) has been shown to regulate IEB permeability. Whether EGCs and GSNO protect the during infectious insult by pathogens such as <i>Shigella flexneri</i> is not known. <h3>Methods</h3> <i>S effects were characterised using in vitro coculture models Caco-2 (or GSNO), ex vivo human colonic mucosa, ligated rabbit loops. The effect...
The transcription factor atonal homolog 1 (ATOH1) controls the fate of intestinal progenitors downstream Notch signaling pathway. Intestinal that escape activation express high levels ATOH1 and commit to a secretory lineage fate, implicating as gatekeeper for differentiation epithelial cells. Although some factors ATOH1, such SPDEF, have been identified specify maturation specific cell types, bona fide transcriptional targets still largely are unknown. Here, we aimed identify likely...
Leucine-rich repeat-containing G-protein coupled receptor 5-expressing (Lgr5(+)) cells have been identified as stem/progenitor in the circumvallate papillae, and single cultured Lgr5(+) give rise to taste cells. Here we use papilla tissue establish a three-dimensional culture system (taste bud organoids) that develops phenotypic characteristics similar native tissue, including multilayered epithelium containing outer layers inner layers. Furthermore, characterization of cell cycle progenitor...
Abstract Background Emerging evidences suggest that enteric glial cells (EGC), a major constituent of the nervous system (ENS), are key regulators intestinal epithelial barrier (IEB) functions. Indeed EGC inhibit (IEC) proliferation and increase IEB paracellular permeability. However, role on other important functions signalling pathways involved in their effects currently unknown. To achieve this goal, we aimed at identifying impact upon IEC transcriptome by performing microarray studies....
L-glutamine (Gln) is a key metabolic fuel for intestinal epithelial cell proliferation and survival may be conditionally essential gut homeostasis during catabolic states. We show that L-alanyl-L-glutamine (Ala-Gln), stable Gln dipeptide, protects mice against jejunal crypt depletion in the setting of dietary protein fat deficiency. Separately, we murine cultures (enteroids) derived from jejunum require or Ala-Gln maximal expansion. Once expanded, enteroids deprived display gradual atrophy...