A5035489054- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Glioma Diagnosis and Treatment
- Sphingolipid Metabolism and Signaling
- Free Radicals and Antioxidants
- Photodynamic Therapy Research Studies
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Fatty Acid Research and Health
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Muscle metabolism and nutrition
- Birth, Development, and Health
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism and Genetic Disorders
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Effects of Radiation Exposure
- Lipid metabolism and biosynthesis
- Pluripotent Stem Cells Research
- Anesthesia and Neurotoxicity Research
Inserm
2016-2025
Nantes Université
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2013-2024
Université d'Angers
2018-2024
La Ligue Contre le Cancer
2013-2017
Sorbonne Paris Cité
2012
Institut Cochin
1997-2012
Université Paris Cité
1997-2012
Délégation Paris 5
1997-2012
Uncoupling protein 2 (UCP2) belongs to the mitochondrial anion carrier family and partially uncouples respiration from ATP synthesis when expressed in recombinant yeast mitochondria. We generated a highly sensitive polyclonal antibody against human UCP2. Its reactivity toward proteins was compared between wild type ucp2(−/−) mice, leading non-ambiguous identification of detected UCP2 spleen, lung, stomach, white adipose tissue. No heart, skeletal muscle, liver, brown The level spleen...
Abstract Cancer cells integrate multiple biosynthetic demands to drive unrestricted proliferation. How these cellular processes crosstalk fuel cancer cell growth is still not fully understood. Here, we uncover the mechanisms by which transcription factor Carbohydrate responsive element binding protein (ChREBP) functions as an oncogene during hepatocellular carcinoma (HCC) development. Mechanistically, ChREBP triggers expression of PI3K regulatory subunit p85α, sustain activity pro-oncogenic...
Uncoupling protein-2 (UCP2) belongs to the mitochondrial carrier family and has been thought be involved in suppressing ROS production through uncoupling respiration from ATP synthesis. However, we show here that loss of function UCP2 does not result a significant increase or an increased propensity for cells undergo senescence culture. Instead, Ucp2-/- display enhanced proliferation associated with metabolic switch fatty acid oxidation glucose metabolism. This requires unrestricted...
Abstract Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of embryonic origin offer potential to generate patient-specific cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, iPSCs (hiPSCs), restricting their post-implantation development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we...
Deficiency of the endoplasmic reticulum (ER) protein seipin results in generalized lipodystrophy by incompletely understood mechanisms. Here, we report mitochondrial abnormalities seipin-deficient patient cells. A subset is enriched at ER-mitochondria contact sites (MAMs) human and mouse cells localizes vicinity calcium regulators SERCA2, IP3R, VDAC. Seipin association with MAM stimulated fasting-like stimuli, while lipid droplets promoted loading. Acute removal does not alter ER stores but...
Abstract Background Glioblastoma (GBM) is a highly aggressive tumor with unmet therapeutic needs, which can be explained by extensive intra-tumoral heterogeneity and plasticity. In this study, we aimed to investigate the specific metabolic features of stem cells (GSC), rare subpopulation involved in growth therapy resistance. Methods We conducted comprehensive analyses primary patient-derived GBM cultures GSC-enriched human cell lines using state-of-the-art molecular, metabolic, phenotypic...
Purpose: Glioblastoma (GBM) is the most common and malignant form of primary human brain tumor in adults, with an average survival at diagnosis 18 months. Metabolism a new attractive therapeutic target cancer; however, little known about metabolic heterogeneity plasticity within GBM tumors. We therefore aimed to investigate phenotyping cultures context molecular provide proof concept for personalized targeting GBM.Experimental Design: have analyzed extensively several using transcriptomics,...
Glioma stem cells are highly resistant to cell death and as such supposed contribute tumor recurrence by eluding anticancer treatments. Here, we show that spheroids contain rat neural (NSCs) or glioma (cancer cells, CSCs) express isoforms 1 2 of pyruvate kinase (PKM1 PKM2); however, the expression PKM2 is considerably higher in spheroids. Silencing enhances both apoptosis differentiation human We establish was implicated spheroid through its interaction with Oct4, a major regulator...
Cancer cells tilt their energy production away from oxidative phosphorylation (OXPHOS) toward glycolysis during malignant progression, even when aerobic metabolism is available. Reversing this phenomenon, known as the Warburg effect, may offer a generalized anticancer strategy. In study, we show that overexpression of mitochondrial membrane transport protein UCP2 in cancer sufficient to restore balance and repress phenotypes. Altered expression glycolytic enzymes mediated effects metabolic...
Abstract Drug resistance limits the therapeutic efficacy in cancers and leads to tumor recurrence through ill-defined mechanisms. Glioblastoma (GBM) are deadliest brain tumors adults. GBM, at diagnosis or after treatment, resistant temozolomide (TMZ), standard chemotherapy. To better understand acquisition of this resistance, we performed a longitudinal study, using combination mathematical models, RNA sequencing, single cell analyses, functional drug assays human glioma line (U251). After...
Abstract Background Osteoarthritis is an age-related disease that currently faces a lack of symptomatic treatment. Inflammation, which mainly sustained by pro-inflammatory cytokines such as IL-1b, TNF, and IL-6, plays important role in osteoarthritis progression. In this context, are widely used to mimic the inflammatory component vitro. However, therapeutic failures clinical trials evaluating anti-cytokines drugs highlight overall understanding effects these on chondrocytes. Methods Here,...
A decreased mobilization of triglycerides may contribute to fat accumulation in adipocytes, leading obesity. However, this hypothesis remains be proven. In study, epinephrine-induced lipid was investigated vivo nine markedly obese children (160+/-5% ideal body weight) aged 12.1+/-0.1 yr during the dynamic phase deposition, compared with six age-matched nonobese children. As an index lipolysis, we measured glycerol flux using a nonradioactive tracer dilution approach, and plasma free fatty...
Abstract Mitochondrial uncoupling of oxidative phosphorylation may serve a variety purposes such as the regulation substrate oxidation, free radical production (a major by‐product mitochondrial respiration) and ATP turnover. As regulators energy expenditure antioxidant defenses, proteins would seem to offer an attractive mechanism by which explain control body weight, resting metabolic rate aging. result, discovery UCP1 homologues has led impressive number publications. However, 10 years...
Abnormal metabolism and the evasion of apoptosis are considered hallmarks cancers. Accumulating evidence shows that cancer stem cells key drivers tumor formation, progression, recurrence. A successful therapy must therefore eliminate these known to be highly resistant apoptosis. In this paper, we describe metabolic changes as well mechanisms resistance occurring in cells, underlying connection between two processes.
Abstract We have recently shown that the in vitro differentiation of human mesenchymal stem cells (hMSCs) was accompanied by an increased sensitivity toward apoptosis; however, mechanism responsible for this shift is not known. Here, we show repair DNA double-strand breaks (DSBs) more rapid undifferentiated hMSCs than differentiated osteoblasts quantification disappearance γ-H2AX foci nuclei after γ-irradiation-induced damage. In addition, there a marked and prolonged increase level nuclear...