A5090091220- Occupational and environmental lung diseases
- Mass Spectrometry Techniques and Applications
- Medical Imaging and Pathology Studies
- Pleural and Pulmonary Diseases
- Analytical chemistry methods development
- Ion-surface interactions and analysis
- Laser-induced spectroscopy and plasma
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Various Chemistry Research Topics
- Carcinogens and Genotoxicity Assessment
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Diamond and Carbon-based Materials Research
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Occupational exposure and asthma
- History and advancements in chemistry
- Surface Roughness and Optical Measurements
- Microfluidic and Capillary Electrophoresis Applications
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Polymer Surface Interaction Studies
- Cytomegalovirus and herpesvirus research
Loughborough University
2013-2024
This review covers developments in the design of Laser Ablation (LA) cells, associated transport tubing assembly, and their coupling to Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) instrumentation.
Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of hematopoietic stem cell (HSC) transplantation, autoimmune disease, and solid organ transplantation. Determining persistence cell-based therapies vivo crucial understanding their regulatory function requires combination an extremely sensitive detection technique stable, long-lifetime labeling agent. This paper reports first application laser ablation inductively coupled plasma mass...
In recent years, laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) has gained increasing importance for biological analysis, where ultratrace imaging at micrometer resolution is required. However, while undoubtedly a valuable research tool, the washout times and sensitivity of current technology have restricted its routine clinical application. Long periods between sampling points are required to maintain adequate spatial resolution. Additionally, temporal signal...
<div>Abstract<p>Pleural mesothelioma is a fatal disease with limited treatment options. Recently, pleural management has improved the development of immune checkpoint inhibitors (ICI). In first-line therapy, dual PD-1 and CTLA-4 blockade enhances tumor control patient survival compared chemotherapy. Unfortunately, only fraction patients responsive to immunotherapy, approaches reshape microenvironment make ICIs more effective are urgently required. this study, we evaluated effect...
<p>Supplementary Figure S5: Impact of HITOC on tumor-infiltrating T cells.</p>
<p>Supplementary Figure S3: Representative flow cytometry histograms.</p>
<p>Supplementary Figure S4: Effect of HITOC on CD4 T cell infiltration in tumors.</p>
<p>Supplementary Figure S1: Evaluation of the impact heat adjunct on cisplatin cytotoxicity.</p>
<p>Supplementary Figure S6: Effect of HITOC on PD-1 and CTLA-4 expression by CD4 T cells.</p>
<p>Supplementary Figure S2: Flow cytometry gating.</p>
Abstract Administering immunoregulatory cells to patients as medicinal agents is a potentially revolutionary approach the treatment of immunologically mediated diseases. Presently, there are no satisfactory, clinically applicable methods tracking human in with adequate spatial resolution and target cell specificity over sufficient period time. Laser ablation–inductively coupled plasma mass spectrometry (LA-ICP-MS) represents potential solution problem detecting very rare tissues. In this...
The introduction of rapid response laser ablation cells and sample transport technologies to inductively coupled plasma mass spectrometry (LA-ICPMS) has enabled signal pulse durations for a single shot less than 10 ms. These developments have resulted in marked improvements analytical throughput, resolution, sensitivity vital the generation large, highly spatially resolved elemental maps. focus on mapping, particularly bioimaging, obscured possibility applying advantage other LA-ICPMS...
The use of a low aerosol dispersion ablation chamber within laser inductively coupled plasma mass spectrometer (LA-ICP–MS) setup allows for high-resolution, high-speed imaging the distribution elements sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report platinum at cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses cisplatin. This revealed first time correlation between...
This work presents the synthesis of five new functionalized (benz)imidazolium
A prototype time-of-flight mass spectrometer and laser ablation chamber have been used together for the first time, providing differentiation between three forms of harmful asbestos fibre in biological samples.
Improving time resolution in sector-field ICP-MS through use of a plug-in data acquisition board.
New LA-ICP-MS imaging software has been developed and applied to perform fast, high spatial resolution of zinc in retina tissue.
Rationale Malignant pleural mesothelioma is an extremely aggressive and incurable malignancy associated with prior exposure to asbestos fibres. Difficulties remain in relation early diagnosis, notably due impeded identification of lung tissue. This study describes a novel laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) imaging approach identify within models clinical significance. Methods Human cells were exposed different types fibres prepared on plastic slides for...
Abstract Pleural mesothelioma is a fatal disease with limited treatment options. Recently, pleural management has improved the development of immune checkpoint inhibitors (ICI). In first-line therapy, dual PD-1 and CTLA-4 blockade enhances tumor control patient survival compared chemotherapy. Unfortunately, only fraction patients responsive to immunotherapy, approaches reshape microenvironment make ICIs more effective are urgently required. this study, we evaluated effect hyperthermic...
A new app, freely available for Windows computers, has been developed to simulate tuning of an inductively coupled plasma mass spectrometer, on the basis optimization data collected using a sector-field instrument. The app allows students adjust parameters, including torch position, gas flows, radio-frequency power, and guard-electrode state, while observing signal three "measured" variables in real time. used with group second-year undergraduate supplement theoretical material taught Atomic...
ABSTRACT Cardiovascular diseases (CVDs) are the leading causes of death worldwide, with approx. Twenty million deaths in 2021. implants among most used biomaterials clinical world. However, poor endothelialisation and rapid thrombosis remains a challenge. Simple chemical surface modification techniques can be to steer biological interactions without affecting bioimplants' overall bulk characteristics such as radiopacity flexibility. Although silanes well studied for protein cell...