Protein adhesion plays a major role in determining the biocompatibility of materials. The first stage implant integration is protein followed by cell attachment. Surface modification implants (surface chemistry and topography) to induce control currently great interest. This communication presents data on adsorption (bovine serum albumin fibrinogen) onto model hydrophobic (CH3) hydrophilic (OH) surfaces, investigated using quartz crystal microbalance (QCM) grazing angle infrared...

Protein adsorption behavior is at the heart of many today's research fields including biotechnology and materials science. With understanding protein-surface interactions, control over conformation orientation immobilized species may ultimately allow tailor-made surfaces to be generated. In this contribution interactions have been examined with particular focus on surface curvature without chemistry effects. Silica spheres diameters in range 15-165 nm both hydrophilic hydrophobic chemistries...

Switching between superhydrophobicity and superhydrophilicity in porous materials was predicted theoretically demonstrated experimentally with the example of thermally induced contact angle change; tunability this system also demonstrated.

Wall adsorption is a common problem in microfluidic devices, particularly when proteins are used. Here we show how superhydrophobic surfaces can be used to reduce protein and promote desorption. Hydrophobic surfaces, both smooth having high surface roughness of varying length scales (to generate superhydrophobicity), were incubated solution. The samples then exposed flow shear device designed simulate environment. Results that similar amount adsorbed onto nanometer-scale rough although...

In this work we present a reliable technique for the production of large areas high aspect-ratio patterns and describe their use as model super-hydrophobic systems. The thickness straight sidewalls possible with SU-8 were used to generate dense small pillars. These photoresist could be directly, without need micromoulding. A method is given allowing resist varied over wide range bottom antireflective layer was simplify patterning on reflective substrates. This allows rapid testing wetting...

Dried plant specimens stored in herbaria are an untapped treasure chest of information on environmental conditions, evolution and change over many hundreds years. Owing to their delicate nature irreplaceability, there is limited access for analysis these sensitive samples, particularly where chemical data obtained using destructive techniques. Fourier transform infrared (FTIR) spectroscopy a technique which can be applied non-destructively understand bonding and, therefore, functional groups...

<ns3:p>The study of <ns3:italic>in vivo</ns3:italic> thrombus formation has principally been performed using intravital microscopy in mice and other species. These have allowed us to visualise the molecular cellular processes that regulate inside body. However current arterial thrombosis models are difficult standardise between labs frequently produce results do not reliably translate successfully human clinical trials. Here we provide a step-by-step description with accompanying video...

In order to determine the most appropriate method of measuring film thickness using imaging XPS, a series thin polymer films have been prepared and analysed spectroscopy. The these has determined peak shape analysis by photoelectron areas compared with bulk reference relative sensitivity factors. These results values obtained ellipsometry. were seen be influenced chemistry film, so that similar is required for accurate measurement. analysis, both interactive non‐interactive, not dependent on...

Retardation through incarceration: The highly strained anti-Bredt bridgehead olefin bicyclo[2.2.2]oct-1-ene (1) is remarkably stable at 60 °C, if generated photochemically inside the hemicarcerand 2 (see picture). surrounding host not only prevents dimerization of 1, but also retards strongly its intramolecular retro-Diels–Alder reaction by several orders magnitude.

Protein adsorption is of major and widespread interest, being useful in the fundamental understanding biological processes at interfaces through to development new materials. A number techniques are commonly used study protein adhesion, but few directly quantitative. Here we describe use Nano Orange, a fluorometric assay, quantitatively assess bovine fibrinogen albumin onto model hydrophilic (OH terminated) hydrophobic (CH3 surfaces. Results obtained using this method allowed calibration...

Recent reports using particle image velocimetry and cone-and-plate rheometers have suggested that a simple Newtonian liquid flowing across superhydrophobic surface demonstrates finite slip length. Slippage on indicates the combination of topography hydrophobicity may consequences for coupling at solid--liquid interface observed high-frequency shear-mode oscillation quartz crystal microbalance (QCM). In this work, we report response 5 MHz QCM possessing to immersion in water--glycerol...

Mesenchymal stem cells (MSCs) immunomodulate inflammatory responses through paracrine signalling, including via secretion of extracellular vesicles (EVs) in the cell secretome. We evaluated therapeutic potential MSCs-derived small EVs an antigen-induced model arthritis (AIA). isolated from MSCs cultured normoxically (21% O2, 5% CO2), hypoxically (2% CO2) or with a pro-inflammatory cytokine cocktail were applied into AIA model. Disease pathology was assessed post-arthritis induction swelling...

This report describes methods for fabricating substrates with anisotropic order from a single solution of high concentration collagen. By exploiting the intrinsic property collagen to behave as cholesteric liquid crystal, we demonstrate first production dense films containing fibers by simple dialysis and polymerization in ammonia vapor. We then utilized shear driven alignment using viscous extrusion produce aligned fibers. Next describe an evaporation technique observe crystalline growth...

Deep tissue penetration of a NIR PSMA-QD655 imaging agent in 3D prostate cancer platform has been achieved.

Fe3O4-Au hybrid nanoparticles (HNPs) have shown increasing potential for biomedical applications such as image guided stimuli responsive drug delivery. Incorporation of the unique properties HNPs into thermally scaffolds holds great future applications. Here we successfully fabricated smart based on thermo-responsive poly(N-isopropylacrylamide) (pNiPAM). Nanoparticles providing localized trigger heating when irradiated with a short laser burst were found to give rise remote control bulk...