A5071424023- Forensic Fingerprint Detection Methods
- Mass Spectrometry Techniques and Applications
- Forensic Toxicology and Drug Analysis
- Forensic and Genetic Research
- Metabolomics and Mass Spectrometry Studies
- Ion-surface interactions and analysis
- COVID-19 Clinical Research Studies
- X-ray Spectroscopy and Fluorescence Analysis
- Dermatological and COVID-19 studies
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Bacterial Genetics and Biotechnology
- Nuclear Physics and Applications
- Analytical Chemistry and Chromatography
- Digital and Cyber Forensics
- Analytical chemistry methods development
- Advanced Proteomics Techniques and Applications
- Isotope Analysis in Ecology
- Integrated Circuits and Semiconductor Failure Analysis
- Long-Term Effects of COVID-19
- Hematological disorders and diagnostics
- Advancements in Transdermal Drug Delivery
- Viral Infections and Outbreaks Research
- Gut microbiota and health
- Schizophrenia research and treatment
University of Surrey
2015-2025
Hospital de São Bernardo
2022
Guildford Hospital
2016-2020
Latent fingerprints provide a potential route to the secure, high throughput and non-invasive detection of drugs abuse.
The radical ring-opening polymerization (RROP) of thionolactones provides access to thioester backbone-functional copolymers but has, date, only been demonstrated on acrylic copolymers. Herein, the thionolactone dibenzo[c,e]oxepane-5-thione (DOT) was subjected azobisisobutyronitrile (AIBN)-initiated free-radical homopolymerization, which produced a thioester-functional homopolymer with glass-transition temperature 95 °C and ability degrade exclusively into predetermined small molecules....
Protein secretion in eukaryotes and prokaryotes involves a universally conserved protein translocation channel formed by the Sec61 complex. Unrelated small-molecule natural products synthetic compounds inhibit with differential effects for different substrates or from organisms, making this promising target therapeutic intervention. To understand mode of inhibition provide insight into molecular mechanism dynamic translocon, we determined structure mammalian inhibited Mycobacterium ulcerans...
The majority of metabolomics studies to date have utilised blood serum or plasma, biofluids that do not necessarily address the full range patient pathologies. Here, correlations between metabolites, salivary metabolites and sebum lipids are studied for first time. 83 COVID-19 positive negative hospitalised participants provided alongside saliva samples analysis by liquid chromatography mass spectrometry. Widespread alterations serum-sebum lipid relationships were observed in versus...
Metabolic flux is the final output of cellular regulation and has been extensively studied for carbon but much less known about nitrogen, which another important building block living organisms. For tuberculosis pathogen, this particularly in informing development effective drugs targeting pathogen's metabolism. Here we performed
Latent fingermarks are invisible to the naked eye and normally require application of a chemical developer followed by an optical imaging step in order visualize ridge detail. If finger deposition is poor, or fingermark aged, it can sometimes be difficult produce image sufficient quality for identification. In this work, we show first time how mass spectrometry (in case time-of-flight secondary ion spectrometry, ToF-SIMS) used enhance partially recovered fingermarks. We three examples obtain...
In this work, we demonstrate the development and first application of nanocapillary sampling followed by analytical flow liquid chromatography-mass spectrometry for single-cell lipidomics. Around 260 lipids were tentatively identified in a single cell, demonstrating remarkable sensitivity. Human pancreatic ductal adenocarcinoma cells (PANC-1) treated with chemotherapeutic drug gemcitabine can be distinguished from controls solely on basis their lipid profiles. Notably, relative abundance...
Single-cell lipidomics enables detailed analysis of the lipidomes cells, but is challenged by small sample volumes, risk background interference and a lack validation data. In this study, we explore effect different sampling variables on lipid profiles single pancreatic cancer detected using liquid chromatography-mass spectrometry (LC-MS). We use automated manual capillary methods to isolate living cells evaluate media, tips, aspiration volume, temperature humidity control. demonstrate that...
Liquid Extraction Surface Analysis (LESA) is a new, high throughput tool for ambient mass spectrometry.
Paper spray mass spectrometry (PS-MS) is a technique that has recently emerged and shown excellent analytical sensitivity to number of drugs in blood. As an alternative blood, fingerprints have been provide noninvasive traceable sampling matrix. Our goal was validate the use fingerprint samples detect cocaine use.Samples were collected on triangular pieces (168 mm2) washed Whatman Grade I chromatography paper. Following application internal standard, solvent voltage applied paper before...
Mephedrone and one of its metabolites have been detected for the first-time in fingerprints collected from a controlled human mephedrone administration study.
Abstract The finding that drugs and metabolites can be detected from fingerprints is of potential relevance to forensic science as well toxicology clinical testing. However, discriminating between dermal contact ingestion has never been verified experimentally. inability interpret the result a drug or metabolite in fingerprint prevented widespread adoption testing limits probative value detecting fingermarks. A commonly held belief detection abuse used confirm ingested. we show here cocaine...
Background The COVID-19 pandemic is likely to represent an ongoing global health issue given the potential for new variants, vaccine escape and low likelihood of eliminating all reservoirs disease. Whilst diagnostic testing has progressed at a fast pace, metabolic drivers outcomes–and whether markers can be found in different biofluids–are not well understood. Recent research shown that serum metabolomics prognosis disease progression. In hospital setting, collection saliva samples more...
Abstract BACKGROUND Recent publications have explored the possibility of using fingerprints to confirm drug use, but none has yet dealt with environmental contamination from fingertips. Here we establishing an cutoff for testing a single fingerprint. METHODS Fingerprint samples (n = 100) were collected hands 50 nondrug users before and after handwashing establish separate values protocols cocaine, benzoylecgonine, heroin, 6-monoacetylmorphine. The was challenged by drug-free volunteers...
Here we show how mass spectrometry imaging can be used to distinguish between contact and administration of cocaine, using single fingerprint samples.
The use of a low aerosol dispersion ablation chamber within laser inductively coupled plasma mass spectrometer (LA-ICP–MS) setup allows for high-resolution, high-speed imaging the distribution elements sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report platinum at cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses cisplatin. This revealed first time correlation between...
Immunoglobulin gene heterogeneity reflects the diversity and focus of humoral immune response towards different infections, enabling inference B cell development processes. Detailed compositional lineage analysis long read IGH repertoire sequencing, combining examples pandemic, epidemic endemic viral infections with control vaccination samples, demonstrates general responses including increased use
Abstract In this publication we work towards providing fast, sensitive and selective analysis of explosive compounds collected on swabs using paper spray mass spectrometry. We have (a) increased the size substrate to 1.6×2.1 cm for compatibility with current practise in swabbing material; (b) developed a method determining successful extraction analyte from reduce false negative events; (c) expanded range analytes that can be detected include peroxide HMTD, as well nitroglycerine (NG),...
Result of measured N-content in test set Ge<sub>2</sub>Sb<sub>2</sub>Te<sub>5</sub>:N samples. XRF calibrated using a reference materials certified by IBA.
Paper spray mass spectrometry is a rapid and sensitive tool for explosives detection but has so far only been demonstrated using high resolution spectrometry, which bears too cost many practical applications. Here we explore the potential paper to be implemented in field applications with portable spectrometry. This involved (a) replacing substrate swabbing material (which call "swab spray") compatibility standard collection materials; (b) of from surfaces; (c) an exploration interferences...
Paper spray offers a rapid screening test without the need for sample preparation. The incomplete extraction of paper allows further testing using more robust, selective and sensitive techniques such as liquid chromatography/mass spectrometry (LC/MS). Here we develop two-step process followed by LC/MS to (1) rapidly screen large number samples (2) confirm any disputed results. This demonstrates applicability medication adherence from fingerprint.
A fingerprint offers a convenient, noninvasive sampling matrix for monitoring therapeutic drug use. However, barrier to widespread adoption of is the fact that sample volume uncontrolled. Fingerprint samples (n = 140) were collected from patients receiving antibiotic isoniazid as part their treatment, well drug-naive control group 50). The analyzed (INH) and acetylisoniazid (AcINH), using liquid chromatography high-resolution mass spectrometry. data set was retrospectively metabolites known...