Parkinson's disease (PD) is a progressive neurodegenerative disorder, which characterised by degeneration of distinct neuronal populations, including dopaminergic neurons the substantia nigra. Here, we use metabolomics profiling approach to identify changes lipids in PD observed sebum, non-invasively available biofluid. We used liquid chromatography-mass spectrometry (LC-MS) analyse 274 samples from participants (80 drug naïve PD, 138 medicated and 56 well matched control subjects) detected...

Parkinson's disease (PD) is a progressive, neurodegenerative that presents with significant motor symptoms, for which there no diagnostic chemical test. We have serendipitously identified hyperosmic individual, "Super Smeller" who can detect PD by odor alone, and our early pilot studies indicated the was present in sebum from skin of subjects. Here, we employed an unbiased approach to investigate volatile metabolites samples obtained noninvasively upper back 64 participants total (21...

Parkinson's disease (PD) is the second most common neurodegenerative disorder, and identification of robust biomarkers to complement clinical diagnosis will accelerate treatment options. Here, we demonstrate use direct infusion sebum from skin swabs using paper spray ionization coupled with ion mobility mass spectrometry (PS-IM-MS) determine regulation molecular classes lipids in that are diagnostic PD. A PS-IM-MS method for samples takes 3 min per swab was developed optimized. The applied...

The majority of metabolomics studies to date have utilised blood serum or plasma, biofluids that do not necessarily address the full range patient pathologies. Here, correlations between metabolites, salivary metabolites and sebum lipids are studied for first time. 83 COVID-19 positive negative hospitalised participants provided alongside saliva samples analysis by liquid chromatography mass spectrometry. Widespread alterations serum-sebum lipid relationships were observed in versus...

Careful transfer of ions into the gas-phase permits measurement protein structures, with ion mobility-mass spectrometry, which provides shape and stoichiometry information. Collision cross sections (CCS) can be obtained from measurements made mobility through a given gas, such structural information once should also permit interlaboratory comparisons. However, until recently, there was not recommended standard form for reporting measurements. In this study, we explore use collision section...

Parkinson's disease (PD) is a progressive neurodegenerative disorder that does not currently have robust clinical diagnostic test. Nonmotor symptoms such as skin disorders long since been associated with the disease, and more recently characteristic odor emanating from of people has identified. Here, dynamic head space (DHS) thermal desorption (TD) gas chromatography-mass spectrometry (GC-MS) implemented to directly measure volatile components sebum on swabs sampled Parkinson's-both drug...

Chromatography-based mass spectrometry approaches (xC-MS) are commonly used in untargeted metabolomics, providing retention time, m/z values and metabolite-specific fragments, all of which to identify validate an unknown analyte. Ion mobility-mass (IM-MS) is emerging as enhancement classic xC-MS strategies, by offering additional ion separation well collision cross section (CCS) determination. In order apply such approach a metabolomics workflow, verified data from metabolite standards...

Abstract Parkinson’s Disease (PD) has been associated with a distinct odour, which emanates from the skin and is strongest in sebum-rich areas. In this study, sebum was sampled participants using cotton gauze volatile components emanating these swabs were analysed directly thermal desorption gas chromatography - mass spectrometry (TD GC-MS). We subjects clinically established PD ( n =46) along healthy controls =28) two sites. The volatilome profiles obtained for control cohorts compared...

Abstract A definitive diagnostic test for PD remains elusive, so identification of potential biomarkers may shed light on methods diagnosis and facilitate early intervention. Excess sebum secretion skin pathology are recognised symptoms PD. It is likely these result in a unique signature volatile organic compounds that could be used to identify stages disease. Numerous medical conditions produce distinctive odours, dogs have been trained detect many these. single previous study, suggested...

Parkinson’s disease (PD) is a progressive, neurodegenerative that presents with significant motor symptoms, for which there no diagnostic test ( 1–3 ). We have serendipitously identified hyperosmic individual, ‘Super Smeller’ can detect PD by odor alone, and our early pilot studies indicated the was present in sebum from skin of subjects( 4 Here, we employed an unbiased approach to investigate volatile metabolites samples obtained non-invasively upper back 64 participants total (21 controls...

<p>A metabolomics profiling approach was conducted to identify diagnostic biomarkers of PD from sebum, a non-invasively available biofluid. In this study, we used liquid chromatography-mass spectrometry (LC-MS) analyse 274 samples participants (80 drug naïve PD, 138 medicated and 56 well matched control subjects) detected metabolites that could predict phenotype. Partial least squares-discriminant analysis (PLS-DA) models based on sebum metabolome had correct classification rates 70.4%...

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sampling facile and non-invasive, its potential use clinical biochemistry assays should be explored including parameters standard operating procedures around collection, transport, storage. To this aim we have here investigated reproducibility mass spectrometry data from relation...

In this study we explore the use of collision cross section distributions to allow comparability IM-MS data for proteins on different instruments. We present measurements seven standard across three configurations, namely an Agilent 6560 IM QToF, a Waters Synapt G2 possessing TWIMS cell and modified RF confining linear field drift cell. Mobility were taken using both He N<sub>2</sub> as gases. To aid instruments best assess corresponding gas-phase conformational landscapes...

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sampling facile and non-invasive, its potential use clinical biochemistry assays should be explored including parameters standard operating procedures around collection, transport, storage. To this aim we have here investigated reproducibility mass spectrometry data from relation...

Sebum is a lipid-rich biofluid produced by the sebaceous glands which are found in high abundance areas where hair present, along neckline, ‘T’-area of face and on upper back. Recent work has shown that sebum can be used mass spectrometry-based metabolomics experimental workflows to identify metabolic changes occur individuals who infected with SARS-CoV-2 virus, Parkinson’s disease REM-sleep Behavioural Disorders (RBD) when compared healthy non-disease controls. We describe here standard...

55'r more so to vie with each other in the common cause of humanity.Then, perhaps, they may come acknowledge truth saying Cicero his oration Pro Li, aiio, " Neque enim ulla alia re homines propijus ad Deos accedant, quam salutem hominibus dando".

In this study we explore the use of collision cross section distributions to allow comparability IM-MS data for proteins on different instruments. We present measurements seven standard across three configurations, namely an Agilent 6560 IM QToF, a Waters Synapt G2 possessing TWIMS cell and modified RF confining linear field drift cell. Mobility were taken using both He N 2 as gases. To aid instruments best assess corresponding gas-phase conformational landscapes protein ‘standards’ in form...

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help stratify progression. Here we demonstrate use paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) determine diagnostic molecular features in sebum. PSI IM-MS was performed directly from skin swabs, collected 34 people 30 matched control subjects as a training set...

<p>Parkinson’s disease (PD) is a progressive neurodegenerative disorder that does not currently have robust clinical diagnostic test. Non-motor symptoms such as skin disorders long since been associated with the and more recently characteristic odour emanating from of people Parkinson’s has identified. Here, dynamic head space (DHS) thermal desorption (TD) gas chromatography-mass spectrometry (GC-MS implemented to directly measure volatile components sebum on swabs sampled – both drug...

A metabolomics profiling approach was conducted to identify diagnostic biomarkers of PD from sebum, a non-invasively available biofluid. In this study, we used liquid chromatography-mass spectrometry (LC-MS) analyse 274 samples participants (80 drug naïve PD, 138 medicated and 56 well matched control subjects) detected metabolites that could predict phenotype. Partial least squares-discriminant analysis (PLS-DA) models based on sebum metabolome had correct classification rates 70.4% 69.7%...

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help stratify progression. Here we demonstrate use paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) determine diagnostic molecular features in sebum. PSI IM-MS was performed directly from skin swabs, collected 34 people 30 matched control subjects as a training set...

Parkinson’s disease (PD) is the second most common neurodegenerative disorder and identification of robust biomarkers to complement clinical diagnosis will accelerate treatment options. Here we demonstrate use direct infusion sebum from skin swabs using paper spray ionisation coupled with ion mobility mass spectrometry (PS-IM-MS) determine regulation molecular classes lipids in that are diagnostic PD. A PS-IM-MS method for samples takes three minutes per swab was developed optimised. The...

Chromatography based mass spectrometry approaches (xC-MS) are commonly used in untargeted metabolomics, providing retention time, m/z values and metabolite specific-fragments all of which to identify validate an unknown analyte. Ion mobility-mass (IM-MS) is emerging as enhancement classic xC-MS strategies, by offering additional separation well collision cross section (CCS) determination. In order apply such approach a synthetic biology workflow, verified data from standards necessary. this...